Modeling of plant in vitro cultures: Overview and estimation of biotechnological processes

Abstract

Plant cell and tissue cultivations are of growing interest for the production of structurally complex and expensive plant-derived products, especially in pharmaceutical production. Problems with up-scaling, low yields, and high-priced process conditions result in an increased demand for models to provide comprehension, simulation, and optimization of production processes. In the last 25 years, many models have evolved in plant biotechnology; the majority of them are specialized models for a few selected products or nutritional conditions. In this article we review, delineate, and discuss the concepts and characteristics of the most commonly used models. Therefore, the authors focus on models for plant suspension and submerged hairy root cultures. The article includes a short overview of modeling and mathematics and integrated parameters, as well as the application scope for each model. The review is meant to help researchers better understand and utilize the numerous models published for plant cultures, and to select the most suitable model for their purposes.

Mass propagation of Helianthus annuus suspension cells in orbitally shaken bioreactors: Improved growth rate in single‐use bag bioreactors

“…In all of the systems, the sucrose was extracellularly split into the monosaccharides glucose and fructose, which were taken up into the cells. This is comparable to previously pusblished results with H. annuus plant cell cultures and it is typical to plant cell cultures under heterotrophic conditions with sucrose as the carbon and energy source . The course of this process is depicted in Fig.…”

Glucosinolate biosynthesis as influenced by growth media and auxin in hairy root cultures of Kale (Brassica oleracea var. acephala)

“…The production of secondary metabolites in hairy root cultures is influenced by many factors, including temperature, light, pH, medium composition, and plant growth regulators (Zhou et al, 2011;Tian, 2015). Hairy root cultures from many plant species have been widely studied for the production of secondary metabolites useful in pharmaceuticals, cosmetics, and food additives (Srivastava and Srivastava, 2007;Ludwig-Müller et al, 2014;Maschke et al, 2015). To our knowledge, glucosinolate production by hairy root cultures of kale has never been reported.…”

Means to optimize protein expression in transgenic plants

“…Individual culture condition factors in plant cell culture or conventional crop systems which directly or indirectly influence protein production are diverse and previous work has covered them comprehensively [69,70]. A recent review summarizes the existing modeling approaches for plant cultures and the parameters that were covered by the models [71]. Recent examples for a successful improvement of culture conditions are the alpha-tocopherol production in Helianthus annuus [72] and biomass measurements in the microalgea Chlorella sp.…”