Modeling of Polyhydroxyalkanoate Synthesis from Biogas by Methylocystis hirsuta

Abstract

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Optimization of nitrogen feeding strategies for improving polyhydroxybutyrate production from biogas by Methylocystis parvus str. OBBP in a stirred tank reactor

“…Microbial PHB was quantified in an Agilent 7820A gas chromatograph (GC) coupled to an Agilent 5977E mass spectrometer (MS) (Agilent Technologies, USA) following hydrochloric acid propanolysis (Rodríguez et al, 2020a). Gas chromatographic conditions are detailed elsewhere (Chen et al, 2020). Biomass concentration was determined as grams of TSS per liter of cell culture according to Standard Methods (2540 Solids, APHA, 2018) using Merck MF Millipore (0.45 μm pore size) membrane filters (Merck, Germany).…”

Structural variability, implementational irregularities in mathematical modelling of polyhydroxyalkanoates (PHAs) production—A state‐of‐the‐art review

“…Activated sludge and multiplicative Monod model have been included to map the influence of oxygen along with nitrogenous and carbonaceous substrates on microbial growth (X. Chen et al, 2020;Fang et al, 2019;Karahan et al, 2008). Oxygen as the sole growth-limiting factor has even been proposed for P. putida assuming a high concentration of carbonaceous feed (Diniz et al, 2004).…”

“…In addition to carbon and nitrogen sources, oxygen can be a growth‐limiting substrate in PHA production as reflected in a handful of studies (Davis et al, 2015; Karasavvas & Chatzidoukas, 2020; Vargas et al, 2014). Activated sludge and multiplicative Monod model have been included to map the influence of oxygen along with nitrogenous and carbonaceous substrates on microbial growth (X. Chen et al, 2020; Fang et al, 2019; Karahan et al, 2008). Oxygen as the sole growth‐limiting factor has even been proposed for P. putida assuming a high concentration of carbonaceous feed (Diniz et al, 2004).…”

“…However, all the above‐referred aspects have rarely been taken into consideration in the dynamic modelling of microbial biopolymer production (Table 3). Only a few studies have implemented a discretization strategy to estimate parameters relevant to biomass growth/decay and PHA synthesis/utilization from independent batch experiments (X. Chen et al, 2020), though confidence intervals or sensitivity of those estimates remains obscure. Model reduction strategies have been adopted in SBR operations treating synthetic wastewater using manipulated operating conditions (Liau et al, 2015).…”

3D printing to enable the reuse of marine plastic waste with reduced environmental impacts

“…In this sense, polyhydroxyalkanoates (PHAs) are polyesters that can be degraded under composting and marine environments with no harmful byproducts, a rather uncommon feature in the family of biopolymers. Polyhydroxybutyrate (PHB), the most widely used polymer within the PHA family, has similar thermomechanical properties than the petro‐based polypropylene and is synthesized via a fermentation process by microorganisms (Chen et al., 2020).…”