2023
DOI: 10.1007/s10577-023-09735-7
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Modeling specific aneuploidies: from karyotype manipulations to biological insights

My Anh Truong,
Paula Cané-Gasull,
Susanne M. A. Lens

Abstract: An abnormal chromosome number, or aneuploidy, underlies developmental disorders and is a common feature of cancer, with different cancer types exhibiting distinct patterns of chromosomal gains and losses. To understand how specific aneuploidies emerge in certain tissues and how they contribute to disease development, various methods have been developed to alter the karyotype of mammalian cells and mice. In this review, we provide an overview of both classic and novel strategies for inducing or selecting specif… Show more

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Cited by 8 publications
(1 citation statement)
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“…More recently, an approach based on the targeting of alpha satellite repeats of 19 out of 24 human centromeres by defective Cas (dCas9) fused to a mutant KLN1 (KaryoCreate) has been reported to be able to create both losses and gains on ten human chromosomes [41]. Tovini and coworkers used a dCas9-CENP-T fusion protein to create new centromeres to cause aneuploidies, including chromosome loss [42], while Truong et al used dCas9 to link microtubule minus-end-directed Kinesin 14VIb to subtelomeric (HSA1) or centromeric (HSA9) repeated sequences to interfere with mitosis, thus causing the loss of the targeted chromosome [43]. Similarly, Girish and coworkers reported the elimination of chromosome 1 from a neoplastic cell line by modification of the HSV-TK and CRISPR strategies [44].…”
Section: Step 3: Chromosome Eliminationmentioning
confidence: 99%
“…More recently, an approach based on the targeting of alpha satellite repeats of 19 out of 24 human centromeres by defective Cas (dCas9) fused to a mutant KLN1 (KaryoCreate) has been reported to be able to create both losses and gains on ten human chromosomes [41]. Tovini and coworkers used a dCas9-CENP-T fusion protein to create new centromeres to cause aneuploidies, including chromosome loss [42], while Truong et al used dCas9 to link microtubule minus-end-directed Kinesin 14VIb to subtelomeric (HSA1) or centromeric (HSA9) repeated sequences to interfere with mitosis, thus causing the loss of the targeted chromosome [43]. Similarly, Girish and coworkers reported the elimination of chromosome 1 from a neoplastic cell line by modification of the HSV-TK and CRISPR strategies [44].…”
Section: Step 3: Chromosome Eliminationmentioning
confidence: 99%