Modeling the Osteogenic Potential of Decellularized Human Bone Particles by Tuning their Size Distribution Through a Sonic Microfragmentation Approach

Abstract

Human decellularized bone fragments are commonly used in clinics to perform allograft surgeries. To reduce the immunological response in the recipient and ensure their safety, these fragments underwent decellularization, a procedure that greatly reduces their osteogenicity (the ability to induce differentiation into osteoblast). In this work, by the introduction of an ultrasonication step to fragment the human bone, the size distribution of the resulting demineralize bone particles can be controlled, tuning their osteogenic potential. The sonication protocol is optimized by a response surface method, using 12 different sonication protocols, allowing to model the relationship between the sonication parameters and the outcoming particles properties in terms of dimensions, physical/chemical properties, and biological activity. The size distribution is extrapolated by a deep learning image segmentation while the structure is characterized by infrared and thermal analysis. The particles are combined with methacrylated silk gel to test in vitro their biological response on adipose‐derived stromal cells. The ultrasonication fragmented the bone particles, revealing their internal organic matrix as proved by secondary electron microscopy and confocal microscopy. An inverse linear correlation is found between the particles’ sizes and their osteogenic activity, thus proving the efficacy of the proposed ultrasonication treatment in tuning the biological response.

A Gellan Gum, Polyethylene Glycol, Hydroxyapatite Composite Scaffold with the Addition of Ginseng Derived Compound K with Possible Applications in Bone Regeneration

“…In several of these cases, the severity of the damage does not allow autonomous bone regeneration, and the patient must be implanted with additional tissue to ensure complete recovery. For example, when bone resorption is no longer compensated for by bone formation, there is a progressive decline in bone mass, ultimately leading to osteoporosis, a major risk factor for fragility fractures [11]. For this purpose, various materials and surgical procedures have been investigated over the years.…”