Modeling the recovery patterns from solid phase extraction purification of secoisolariciresinol diglucoside, p-coumaric acid glucoside, and ferulic acid glucoside from microwave-assisted flaxseed extracts

Nemes, Simona M.; Orsat, Valérie

doi:10.1016/j.fbp.2011.11.003

Cited by 9 publications

(7 citation statements)

References 15 publications

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“…6−8 However, extraction and purification of lignans from the flaxseed hull is costly. 12,13 Lignan-enriched products (e.g., SDG polymer) pose a more economical alternative and would, therefore, be attractive if such products demonstrated similar efficacy as compared to a nutraceutical dosage form. Dosage and efficacy of SDG polymer is dependent upon the efficiency from which SDG is released from the polymer matrix.…”

Section: ■ Results and Discussionmentioning

confidence: 99%

“…Blood samples (300 μL per time) of the first subgroup (n = 6) were collected via jugular vein cannula at 0, 0.25, 0.5, 1, 1.5, 2, 3, 4, 8, and 12 h after dosing. For the second subgroup (n = 6), jugular blood samples (300 μL) were collected at 0 (predose), 12,16,20,24,32,40, and 48 h after dosing. Rats were provided food 2 h after dosing.…”

Section: ■ Experimental Sectionmentioning

confidence: 99%

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Oral Pharmacokinetics of Enriched Secoisolariciresinol Diglucoside and Its Polymer in Rats

et al. 2021

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Secoisolariciresinol diglucoside (SDG) is the principal lignan of flaxseed and precursor of its aglycone, secoisolariciresinol (SECO), and the mammalian lignans enterolactone (EL) and enterodiol (ED), the putative bioactive forms of oral administration of SDG. SDG is present in the seed hull as an ester-linked polymer. Although extraction and purification of SDG monomer is costly, the use of naturally occurring SDG in polymer form may offer a more economical approach for delivery of this precursor. The extent of SDG release from the polymer and subsequent bioavailability of SDG metabolites are unknown. To understand the relative bioavailability of SDG polymer, this study examined the comparative bioavailability of enriched SDG and SDG polymer in rats after a single oral SDG equivalent dose (40 mg/kg). A validated LC-MS/MS method quantified SDG and its metabolites in rat plasma following serial blood collections. SDG remained undetectable in rat plasma samples. Unconjugated SECO was detected in plasma after 0.25 h. Unconjugated ED was observed after 8 h (3.4 ± 3.3 ng/mL) and 12 h (6.2 ± 3.3 ng/mL) for enriched SDG and SDG polymer, respectively. Total (conjugated and unconjugated) ED and EL resulting from enriched SDG and SDG polymer reached similar maximal concentrations between 11 and 12 h and demonstrated similar total body exposures (AUC values). These data suggest a similar pharmacokinetic profile between the enriched and polymer form of SDG, providing support for the use of SDG polymer as a more economical precursor for SECO, ED, and EL in applications of chronic disease management.

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Section: ■ Results and Discussionmentioning

confidence: 99%

Section: ■ Experimental Sectionmentioning

confidence: 99%

Oral Pharmacokinetics of Enriched Secoisolariciresinol Diglucoside and Its Polymer in Rats

et al. 2021

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“…Method 1 used a C 18 cartridge; this type of cartridge has been widely used in phenolic compound separation, although it shows low recovery for particular phenols . However, differences in the recovery of low molecular weight polar compounds (4CA and FA) from a C 18 cartridge were not noted in a more recent study …”

Section: Resultsmentioning

confidence: 99%

“…33 However, differences in the recovery of low molecular weight polar compounds (4CA and FA) from a C 18 cartridge were not noted in a more recent study. 34 A noncrossover significant location × method interaction was found for 4CA levels in the rind tissues (Table 2). For the sample from Pontevedra, the 4CA level was 10.1 mg/g as determined by method 1 and 17.4 mg/g as determined by method 2.…”

Section: Journal Of Agricultural and Food Chemistrymentioning

confidence: 94%

Methods for Determining Cell Wall-Bound Phenolics in Maize Stem Tissues

Santiago

Lozano

Souto

et al. 2018

J. Agric. Food Chem.

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We compared two methods with different sample pretreatment, hydrolysis, and separation procedures to extract cell wall-bound phenolics. The samples were pith and rind tissues from six maize inbred lines reportedly containing different levels of cell wall-bound phenolics. In method 1, pretreated samples were extracted with a C solid-phase extraction cartridge, and it took 6 days to complete. In method 2, phenolics were extracted from crude samples with ethyl acetate, it took 2 days to complete, and the cost per sample was reduced more than 60%. Both methods extracted more 4-coumarate than ferulate. Overall, method 1 yielded more 4-coumarate, while method 2 yielded more ferulate. The lack of a genotype × method interaction and significant correlations between the results obtained using the two methods indicate that both methods are reliable for use in large-scale plant breeding programs. Method 2, scaled, is proposed for general plant biology research.

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“…MAE allows for simplified designing, efficient and economical pilot scale studies, and also has a positive impact on future purification studies (Neme and Orsat 2012). In this study, the calculation of the optimal MAE conditions was further carried out according to the RSM model equation.…”

Section: Response Surface Optimization Of Mae Conditionsmentioning

confidence: 99%

Microwave-assisted extraction and purification of chlorogenic acid from by-products of Eucommia Ulmoides Oliver and its potential anti-tumor activity

et al. 2014

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An efficient method for the rapid extraction, separation and purification of chlorogenic acid (CGA) from byproducts of Eucommia Ulmoides Oliver (E. ulmoides) by microwave-assisted extraction (MAE) coupled with highspeed counter-current chromatography (HSCCC) was developed. The optimal MAE parameters were evaluated by response surface methodology (RSM), and they were extraction time of 12 min, microwave power of 420 W, ethanol concentration of 75 %, solvent/sample ratio of 30:1 (mL/g), yield of CGA reached 3.59 %. The crude extract was separated and purified directly by HSCCC using ethyl acetate-butyl alcoholwater (3:1:4, v/v) as the two-phase solvent system. The 14.5 mg of CGA with the purity of 98.7 % was obtained in one-step separation from 400 mg of crude extract. The chemical structure of CGA was verified with IR, ESI-MS analysis. Meanwhile, the purified CGA extract was evaluated by MTT assay and results indicate that CGA extract exhibited potential anti-tumor activity for AGS gastric cancer cell.

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Modeling the recovery patterns from solid phase extraction purification of secoisolariciresinol diglucoside, p-coumaric acid glucoside, and ferulic acid glucoside from microwave-assisted flaxseed extracts

Cited by 9 publications

References 15 publications

Oral Pharmacokinetics of Enriched Secoisolariciresinol Diglucoside and Its Polymer in Rats

Oral Pharmacokinetics of Enriched Secoisolariciresinol Diglucoside and Its Polymer in Rats

Methods for Determining Cell Wall-Bound Phenolics in Maize Stem Tissues

Microwave-assisted extraction and purification of chlorogenic acid from by-products of Eucommia Ulmoides Oliver and its potential anti-tumor activity

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