In recent days, Web 2.0 has become a popular tool including free digital programs which is utilized to create and share student-created projects and products. They are cooperative, multipurpose, easier to utilize digital platforms which boost students for interacting with others or generate and share individualized response products. At the same time, web accessibility checking tools play a vital role in Web 2.0. Therefore, this article aims to investigate the performance of the accessibility checker (AChecker) tool for education and learning websites in Web 2.0. The AChecker validates HTML content for web accessibility issues by providing the location of the web page, uploading an HTML file, or pasting the whole HTML source code from a website. It generates a report of every web accessibility problem for the chosen guidelines for the known, likely, and potential problems. In addition, the AChecker tool is used to examine a set of 25 websites related to education and learning. Brief performance analysis of the AChecker tool takes place using WCAG 1.0 and WCAG 2.0 guidelines. The experimental outcome indicated that the websites are required to be improved for accessibility by everyone.