Modeling Tissue Morphogenesis and Cancer in 3D

Yamada, Katsushi; Cukierman, Edna

doi:10.1016/j.cell.2007.08.006

Cited by 1,560 publications

(1,360 citation statements)

References 86 publications

Supporting

Mentioning

1,327

Contrasting

Unclassified

Order By: Relevance

“…There are several excellent reviews on the value of 3-D in vitro models (Schmeichel and Bissell, 2003;Debnath and Brugge, 2005;Yamada and Cukierman, 2007) …”

Section: Commentary Background Informationmentioning

confidence: 99%

Visualizing Protease Activity in Living Cells: From Two Dimensions to Four Dimensions

et al. 2008

View full text Add to dashboard Cite

Proteolytic degradation of extracellular matrix (ECM) components by cells is an important metabolic activity as cells grow, remodel, and migrate through the ECM. The ability to analyze ECM degradation can be valuable in the study of developmental processes as well as pathologies, such as cancer. In this unit we describe an in vitro live cell–based method to image and quantitatively measure the degradation of ECM components by live cells. Cells are grown in the presence of fluorescent dye‐quenched protein substrates (DQ‐gelatin, DQ‐collagen I, and DQ‐collagen IV) that are mixed with protein matrices. Upon proteolytic cleavage, fluorescence is released that directly reflects the level of proteolysis by the cells. Using confocal microscopy and advanced imaging software, the fluorescence is detected and accurate measurements of proteolytic degradation in three and four dimensions can be assessed. Curr. Protoc. Cell Biol. 39:4.20.1‐4.20.15. © 2008 by John Wiley & Sons, Inc.

show abstract

“…There are several excellent reviews on the value of 3-D in vitro models (Schmeichel and Bissell, 2003;Debnath and Brugge, 2005;Yamada and Cukierman, 2007) …”

Section: Commentary Background Informationmentioning

confidence: 99%

Visualizing Protease Activity in Living Cells: From Two Dimensions to Four Dimensions

et al. 2008

View full text Add to dashboard Cite

show abstract

“…substrates may be opaque) [10][11][12][13][14][15][16]. Proper formation of tissue requires 3D interaction between different cell types and mechanical load [3,4,7,[15][16][17]. Therefore, cells need to be grown within 3D matrices comprised of novel biomaterials that mimic in-vivo scaffolds [16,18,19].…”

Section: Biophotonicsmentioning

confidence: 99%

“…Although valuable insights into cell migration have been gained from 2D studies into cell movement, cells in vivo undergo morphogenesis and migrate in 3D. A complete understanding of cell behavior therefore necessitates noninvasive monitoring of cells in 3D over time [4,14,20]. Such studies of chemotaxis in 3D time-lapse can also aid the understanding of many important in-vivo processes, for example wound healing and function of the immune system; and offer insight into disease states involving aberrant cell migration such as cancer metastasis [2][3][4]17].…”

Section: Biophotonicsmentioning

confidence: 99%

“…Cell motility and chemotaxis are critical during embryonic and foetal development; and tissue growth and remodelling occur throughout life requiring regulated cell migration [1][2][3][4]. In adult health, related processes are an important aspect of new clinical technologies for tissue engineering, repair and replacement of damaged organs [4][5][6][7].…”

Section: Introductionmentioning

confidence: 99%

“…In adult health, related processes are an important aspect of new clinical technologies for tissue engineering, repair and replacement of damaged organs [4][5][6][7]. Conventional studies of cell migration have largely relied on the …”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Three‐ and four‐dimensional visualization of cell migration using optical coherence tomography

Rey

Považay

Höfer

et al. 2009

Journal of Biophotonics

View full text Add to dashboard Cite

Conventionally, cell chemotaxis is studied on two‐dimensional (2D) transparent surfaces, due to limitations in optical and image data‐collection techniques. However, surfaces that more closely mimic the natural environment of cells are often opaque. Optical coherence tomography (OCT) is a noninvasive label‐free imaging technique, which offers the potential to visualize moving cells on opaque surfaces and in three dimensions (3D). Here, we demonstrate that OCT is an effective means of time‐lapse videomicroscopy of Dictyostelium cells undergoing 3D (2D+time) cell migration on nitrocellulose substrates and 4D (3D+time) chemotaxis within low‐density agarose gels. The generated image sequences are compatible with current computer‐based image‐analysis software for quantification of cell motility. This demonstrates the utility of OCT for cell tracking and analysis of cell chemotaxis in complex environments. (© 2009 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)

show abstract

3‐D Extracellular Matrix from Sectioned Human Tissues

2014

Self Cite

View full text Add to dashboard Cite

Cell adhesion, migration, and signaling in physiologically normal and pathological processes depend highly on the extracellular matrix that the cell interacts with. A variety of in vitro models of two‐dimensional and three‐dimensional extracellular matrices have been developed to study multiple aspects of cellular behavior. However, there is a profound need for in vitro models of extracellular matrices to closely mimic both biochemical and physical aspects of a three‐dimensional in vivo cellular environment. This unit outlines the preparation of human‐tissue‐derived, cell‐free, three‐dimensional extracellular matrices for studying cellular behavior and cell‐extracellular matrix interactions ex vivo. These protocols can be used to prepare cell‐free matrices from a variety of normal and cancerous tissues. This unit also provides protocols for quality control of acellular matrix preparations, and for immunostaining of cells for specific cellular proteins as well as of extracellular matrices for their components. Curr. Protoc. Cell Biol. 62:19.16.1‐19.16.20. © 2014 by John Wiley & Sons, Inc.

show abstract

Modeling Tissue Morphogenesis and Cancer in 3D

Cited by 1,560 publications

References 86 publications

Visualizing Protease Activity in Living Cells: From Two Dimensions to Four Dimensions

Visualizing Protease Activity in Living Cells: From Two Dimensions to Four Dimensions

Three‐ and four‐dimensional visualization of cell migration using optical coherence tomography

3‐D Extracellular Matrix from Sectioned Human Tissues

Contact Info

Product

Resources

About