Modelling and analysis of a gene-regulatory feed-forward loop with basal expression of the second regulator

Roselius, Louisa; Langemann, Dirk; Müller, Johannes; Hense, Burkhard A.; Filges, Stefan; Jahn, Dieter; Münch, Richard

doi:10.1016/j.jtbi.2014.08.043

Cited by 5 publications

(3 citation statements)

References 31 publications

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“…5a ). Interestingly, integration of multiple FFLs and utilization of feedback loop were discovered to control GlcNAc transport, antibiotics production, and SCB1 synthesis, which would benefit a stable gene expression 52 53 and permit S. coelicolor to show robust adaptation to stimulus. Hence various strategies are used by S. coelicolor to adapt to chemical signals and to deal with fluctuating conditions in different environments.…”

Section: Discussionmentioning

confidence: 99%

ScbR- and ScbR2-mediated signal transduction networks coordinate complex physiological responses in Streptomyces coelicolor

Wang

et al. 2015

Sci Rep

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In model organism Streptomyces coelicolor, γ-butyrolactones (GBLs) and antibiotics were recognized as signalling molecules playing fundamental roles in intra- and interspecies communications. To dissect the GBL and antibiotic signalling networks systematically, the in vivo targets of their respective receptors ScbR and ScbR2 were identified on a genome scale by ChIP-seq. These identified targets encompass many that are known to play important roles in diverse cellular processes (e.g. gap1, pyk2, afsK, nagE2, cdaR, cprA, cprB, absA1, actII-orf4, redZ, atrA, rpsL and sigR), and they formed regulatory cascades, sub-networks and feedforward loops to elaborately control key metabolite processes, including primary and secondary metabolism, morphological differentiation and stress response. Moreover, interplay among ScbR, ScbR2 and other regulators revealed intricate cross talks between signalling pathways triggered by GBLs, antibiotics, nutrient availability and stress. Our work provides a global view on the specific responses that could be triggered by GBL and antibiotic signals in S. coelicolor, among which the main echo was the change of production profile of endogenous antibiotics and antibiotic signals manifested a role to enhance bacterial stress tolerance as well, shedding new light on GBL and antibiotic signalling networks widespread among streptomycetes.

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Section: Discussionmentioning

confidence: 99%

ScbR- and ScbR2-mediated signal transduction networks coordinate complex physiological responses in Streptomyces coelicolor

Wang

et al. 2015

Sci Rep

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“…That is because the second regulator must be sufficiently accumulated before activating the transcription of the target gene, helping the organism to avoid unwanted responses to the transient changing conditions. 57 In contrast to coherent FFLs type I, incoherent type I FFLs can induce transient response to signals through fast activation and delayed inhibition of the target gene. 58 Inspecting the constructed regulatory network in E. coli indicate five cases of coherent FFL type I (four cases involve RpoS as a global regulator).…”

Section: Discussionmentioning

confidence: 99%

Co-expressional conservation in virulence and stress related genes of three Gammaproteobacterial species: Escherichia coli, Salmonella enterica and Pseudomonas aeruginosa

et al. 2015

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Gene co-expression analysis is one of the main aspects of systems biology that uses high-throughput gene expression data. In the present study we applied cross-species co-expressional analysis on a module of biofilm and stress response associated genes. We addressed different kinds of stresses in three most intensively studied members of Gammaproteobacteria including Escherichia coli K12, Pseudomonas aeruginosa PAO1 and Salmonella enterica for which large sets of gene expression data are available. Our aim was to evaluate the presence of common stress response strategies adopted by these microorganisms that may be assigned to the other members of Gammaproteobacteria. Results of functional annotation analysis revealed distinct categories among co-expressed genes, most of which concerned biological processes associated with virulence and stress response. Transcriptional regulatory analysis of genes present in co-expressed modules showed that the global stress sigma factor, RpoS, besides several local transcription factors accounts for the observed co-expressional response, and that several cases of feed-forward loops exist between global regulators, local transcription factors and their targets. Our results lend partial support to our underlying assumption of the conservation of core biological processes and regulatory interactions among these related Gammaproteobacteria members. This has led to the implementation of transferring gene function annotations from well-studied Gammaproteobacterial species to less-characterized members. These findings can shed light on the discovery of new drug targets capable of controlling severe infections caused by these groups of bacteria.

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“…From the time-course data of protein expression, we use a cost-benefit model to understand the cellular fitness, as a result of the gene expression dynamics ( Dekel and Alon, 2005 ; Zaslaver et al, 2006b ; Roselius et al, 2014 ; Fritz et al, 2019 ). In this formulation, the benefit conferred to the cell by the target protein T2 is given by the following expression:…”

Section: Methodsmentioning

confidence: 99%

Experimental Evolution of Anticipatory Regulation in Escherichia coli

et al. 2022

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Environmental cues in an ecological niche are often temporal in nature. For instance, in temperate climates, temperature is higher in daytime compared to during night. In response to these temporal cues, bacteria have been known to exhibit anticipatory regulation, whereby triggering response to a yet to appear cue. Such an anticipatory response in known to enhance Darwinian fitness, and hence, is likely an important feature of regulatory networks in microorganisms. However, the conditions under which an anticipatory response evolves as an adaptive response are not known. In this work, we develop a quantitative model to study response of a population to two temporal environmental cues, and predict variables which are likely important for evolution of anticipatory regulatory response. We follow this with experimental evolution of Escherichia coli in alternating environments of rhamnose and paraquat for ∼850 generations. We demonstrate that growth in this cyclical environment leads to evolution of anticipatory regulation. As a result, pre-exposure to rhamnose leads to a greater fitness in paraquat environment. Genome sequencing reveals that this anticipatory regulation is encoded via mutations in global regulators. Overall, our study contributes to understanding of how environment shapes the topology of regulatory networks in an organism.

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Modelling and analysis of a gene-regulatory feed-forward loop with basal expression of the second regulator

Cited by 5 publications

References 31 publications

ScbR- and ScbR2-mediated signal transduction networks coordinate complex physiological responses in Streptomyces coelicolor

ScbR- and ScbR2-mediated signal transduction networks coordinate complex physiological responses in Streptomyces coelicolor

Co-expressional conservation in virulence and stress related genes of three Gammaproteobacterial species: Escherichia coli, Salmonella enterica and Pseudomonas aeruginosa

Experimental Evolution of Anticipatory Regulation in Escherichia coli

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