Modelling and analysis of the continuous affinity-recycle extraction process: a case of specific elution with low molecular weight competitive inhibitor

Sun, Yan; Xue, J. L.; Dong, Xiaoyan

doi:10.1007/bf00367255

Cited by 5 publications

(4 citation statements)

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“…In (8), the value of the denominator for lysozyme and BSA is equal, while the coefficient of the numerator for lysozyme (a 1 =568) is 45 times larger than that for BSA (a 2 =12.5). This implies that lysozyme is more competitive than BSA in binding to the blue TKS gel.…”

Section: Adsorption Of Proteins To Blue Tsk Gelmentioning

confidence: 99%

“…In the adsorption step, 290 ml of the feedstock containing 0.2 mg/ml lysozyme and 0.8 mg/ml BSA were introduced into the system. To describe the competition of lysozyme with BSA for the accessible CB ligands on the blue TSK gel, the extended Langmuir isotherm (8) was employed in the calculation. Similarly to Sect.…”

Section: 3mentioning

confidence: 99%

“…broth feeding (selective adsorption), contaminant washing and desired protein elution. Affinity filtration shows great potential for application in large-scale and continuous processes [8,9]. Another important advantage of affinity filtration over affinity chromatography is its ability to process a crude broth without a prior clarification step.…”

Section: Introductionmentioning

confidence: 99%

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Purification of lysozyme by multistage affinity filtration

Sun

2002

Bioprocess and Biosystems Engineering

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A multistage affinity filtration process was developed for the purification of proteins. An affinity adsorbent was prepared by immobilizing Cibacron Blue 3GA to TSK gel HW-65F. Adsorption equilibrium experiments showed that the blue TSK gel had a high affinity for lysozyme, while its binding to bovine serum albumin (BSA) was weaker. Using a three-stage affinity filtration system, lysozyme was purified from a model system (a mixture of lysozyme and BSA) and a natural source (chicken egg white). From the chicken egg white, the three-stage affinity filtration increased the recovery yield of lysozyme from 61 to 96%, compared with the one-stage process. A mathematical model taking into account the film and interior diffusions of protein and eluant was developed for the modeling and analysis of the experimental data. Both the experimental and modeling results indicate that the multistage affinity filtration technique can be employed for the selective recovery of proteins.

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Section: Adsorption Of Proteins To Blue Tsk Gelmentioning

confidence: 99%

Section: 3mentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Purification of lysozyme by multistage affinity filtration

Sun

2002

Bioprocess and Biosystems Engineering

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“…As in conventional affinity chromatography, an affinity filtration process is composed of three steps, i.e., broth feeding (adsorption), contaminant washing, and desired protein elution. A key element in the development of such a purification technique is the process modeling, not only for experimental data analysis but also for the process design and optimization (Sun et al, 1995, 1997; Dong et al, 1996). Recently, Herak and Merrill (1989, 1990) used a lumped kinetic model to describe the affinity cross‐flow filtration.…”

Section: Introductionmentioning

confidence: 99%

Modeling and Analysis of the Affinity Filtration Process, Including Broth Feeding, Washing, and Elution Steps

Dong

Sun

1998

Biotechnology Progress

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Affinity filtration is a developing protein purification technique that combines the high selectivity of affinity chromatography and the high processing speed of membrane filtration. In this work a lumped kinetic model was developed to describe the whole affinity filtration process, including broth feeding, contaminant washing, and elution steps. Affinity filtration experiments were conducted to evaluate the model using bovine serum albumin as a model protein and a highly substituted Blue Sepharose as an affinity adsorbent. The model with nonadjustable parameters agreed fairly to the experimental results. Thus, the performance of the affinity filtration in processing a crude broth containing contaminant proteins was analyzed by computer simulations using the lumped model. The simulation results show that there is an optimal protein loading for obtaining the maximum recovery yield of the desired protein with a constant purity at each operating condition. The concentration of a crude broth is beneficial in increasing the recovery yield of the desired protein. Using a constant amount of the affinity adsorbent, the recovery yield can be enhanced by decreasing the solution volume in the stirred tank due to the increase of the adsorbent weight fraction. It was found that the lumped kinetic model was simple and useful in analyzing the whole affinity filtration process.

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Enzyme Production

Illanes

2008

Enzyme Biocatalysis

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Modelling and analysis of the continuous affinity-recycle extraction process: a case of specific elution with low molecular weight competitive inhibitor

Cited by 5 publications

References 8 publications

Purification of lysozyme by multistage affinity filtration

Purification of lysozyme by multistage affinity filtration

Modeling and Analysis of the Affinity Filtration Process, Including Broth Feeding, Washing, and Elution Steps

Enzyme Production

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