“…Endospore-free suspensions of vegetative cells were prepared using a double-pass method and harvesting cells during the early exponential phase prior to the appearance of endospores (Clavel, Carlin, Lairon, Nguyen-The, & Schmitt, 2004). Pure suspensions of endospores were generated according to the method of Gaillard, Leguerinel, and Mafart (1998). This procedure yielded endospore suspensions of >95% purity in terms of phase bright bodies when examined using a phase contrast microscope.…”