Modelling constant potential amperometry for investigations of dopaminergic neurotransmission kinetics in vivo

Schönfuss, D.; Reum, Torsten; Olshausen, Philipp von; Fischer, Tom T.; Morgenstern, Rudolf

doi:10.1016/s0165-0270(01)00465-4

Cited by 34 publications

(30 citation statements)

References 27 publications

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“…1c), a delay in the amperometric response is seen. The region adjacent to the electrode, termed the apparent diffusion distance (length d app ), includes uptake sites to be consistent with previous models (Schmitz et al 2001;Schönfuß et al 2001). If the effect of the catalytic reaction of DOQ and ascorbic acid is neglected (Venton et al 2002), a shorter time to peak following cessation of stimulation (indicated by the horizontal bracket) would be erroneously predicted (Fig.…”

supporting

confidence: 70%

“…Journal of Neurochemistry, 2003Neurochemistry, , 87, 1284Neurochemistry, -1295Neurochemistry, doi:10.1046Neurochemistry, /j.1471Neurochemistry, -4159.2003 Schönfuß et al (2001) to address these questions about dopamine volume transmission. Amperometric detection was used because of its high temporal resolution.…”

Section: Introductionmentioning

confidence: 99%

“…Address correspondence and reprint requests to R. Mark Wightman, CB 3290 Venable Hall, Chapel Hill, NC 27599-3290, USA. E-mail: rmw@unc.edu Abbreviations used: BAN, basolateral amygdaloid nucleus, CP, caudate-putamen, DAT, dopamine transporter, DOQ, dopamine-o-quinone; GAD, glutamic acid decarboxylase, PBS, phosphate-buffered saline; SN/VTA, substantia nigra/ventral tegmental area.Journal of Neurochemistry, 2003Neurochemistry, , 87, 1284Neurochemistry, -1295Neurochemistry, doi:10.1046Neurochemistry, /j.1471Neurochemistry, -4159.2003 Schönfuß et al (2001) to address these questions about dopamine volume transmission. Amperometric detection was used because of its high temporal resolution.…”

mentioning

confidence: 99%

“…To answer such questions, mathematical models that consider the rates of release, uptake and coupled diffusion are required. Although several models have been developed (Nicholson 1995;Cragg et al 2001;Schmitz et al 2001;Schönfuß et al 2001), they have been used only to model exogenous dopamine or stimulated release, and have not been extended to make neurochemical predictions.…”

mentioning

confidence: 99%

“…In this work, we adapted the models of Schmitz et al (2001) and Schönfuß et al (2001) to address these questions about dopamine volume transmission. Amperometric detection was used because of its high temporal resolution.…”

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confidence: 99%

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Real‐time decoding of dopamine concentration changes in the caudate–putamen during tonic and phasic firing

Venton¹,

Zhang

Garris

et al. 2003

Journal of Neurochemistry

251

206

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The fundamental process that underlies volume transmission in the brain is the extracellular diffusion of neurotransmitters from release sites to distal target cells. Dopaminergic neurons display a range of activity states, from low-frequency tonic firing to bursts of high-frequency action potentials (phasic firing). However, it is not clear how this activity affects volume transmission on a subsecond time scale. To evaluate this, we developed a finite-difference model that predicts the lifetime and diffusion of dopamine in brain tissue. We first used this model to decode in vivo amperometric measurements of electrically evoked dopamine, and obtained rate constants for release and uptake as well as the extent of diffusion. Accurate predictions were made under a variety of conditions including different regions, different stimulation parameters and with uptake inhibited. Second, we used the decoded rate constants to predict how heterogeneity of dopamine release and uptake sites would affect dopamine concentration fluctuations during different activity states in the absence of an electrode. These simulations show that synchronous phasic firing can produce spatially and temporally heterogeneous concentration profiles whereas asynchronous tonic firing elicits uniform, steady-state dopamine concentrations. Keywords: amperometry, caudate-putamen, cocaine, diffusion, steady state, volume transmission. J. Neurochem. (2003Neurochem. ( ) 87, 1284Neurochem. ( -1295 Dopaminergic neurons fire in a low-frequency tonic mode and periodically exhibit bursts of high-frequency action potentials . Microdialysis studies have revealed that tonic dopamine concentrations are in the low nanomolar range (Justice 1993). Recently, naturally occuring increases in dopamine concentration have been detected on a subsecond time scale with carbon-fiber electrodes Phillips et al. 2003). These transients appear to arise from phasic firing because they are mimicked by high-frequency electrical stimulation of dopaminergic cell bodies. Like the phasic firing of dopaminergic neurons that occurs during salient stimuli (Schultz 1998), the dopamine transients can be matched to specific behaviors such as interaction with another animal (Robinson et al. 2001 or lever pressing to self-administer cocaine (Phillips et al. 2003).It is well documented that dopamine in the striatum communicates via volume transmission (Garris et al. 1994) which differs from the classic synaptic mode of wiring transmission because neurotransmitter can diffuse to target cells distant from release sites (Zoli et al. 1998;Vizi 2000). The existence of naturally occuring dopamine concentration transients in the brain raises several questions. Are there temporal differences in dopamine concentrations at release and target sites? Is dopamine volume transmission differentially affected by tonic and phasic firing patterns? To answer such questions, mathematical models that consider the rates of release, uptake and coupled diffusion are required. Although several models have been d...

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supporting

confidence: 70%

Section: Introductionmentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

See 3 more Smart Citations

Real‐time decoding of dopamine concentration changes in the caudate–putamen during tonic and phasic firing

Venton¹,

Zhang

Garris

et al. 2003

Journal of Neurochemistry

251

206

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Electrochemical Probes for Detection and Analysis of Exocytosis and Vesicles

2010

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Unraveling the mechanistic details of neurotransmitter exocytosis is arguably among the most important molecular problems in neuroscience today. Investigations at single cells, particularly with electrochemical methods, have given unique chemical and biological insight into this process at the fundamental level. The rapid response time (submillisecond) of microelectrodes makes them well suited for monitoring the dynamic process of exocytosis. We review here recent developments in electrochemical techniques to spatially and simultaneously detect exocytosis across a single cell and to measure the transmitter content of single vesicles removed from cells. The former method is used to demonstrate dynamic heterogeneity in release across a cell, and in the latter work comparison is made between vesicle content and release to conclude that only a fraction of the transmitter is released during full exocytosis.

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Spiking neurons, dopamine, and plasticity: Timing is everything, but concentration also matters

Thivierge

Rivest

Monchi

2007

Synapse

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While both dopamine (DA) fluctuations and spike-timing-dependent plasticity (STDP) are known to influence long-term corticostriatal plasticity, little attention has been devoted to the interaction between these two fundamental mechanisms. Here, a theoretical framework is proposed to account for experimental results specifying the role of presynaptic activation, postsynaptic activation, and concentrations of extracellular DA in synaptic plasticity. Our starting point was an explicitly-implemented multiplicative rule linking STDP to Michaelis-Menton equations that models the dynamics of extracellular DA fluctuations. This rule captures a wide range of results on conditions leading to long-term potentiation and depression in simulations that manipulate the frequency of induced corticostriatal stimulation and DA release. A well-documented biphasic function relating DA concentrations to synaptic plasticity emerges naturally from simulations involving a multiplicative rule linking DA and neural activity. This biphasic function is found consistently across different neural coding schemes employed (voltage-based vs. spike-based models). By comparison, an additive rule fails to capture these results. The proposed framework is the first to generate testable predictions on the dual influence of DA concentrations and STDP on long-term plasticity, suggesting a way in which the biphasic influence of DA concentrations can modulate the direction and magnitude of change induced by STDP, and raising the possibility that DA concentrations may inverse the LTP/LTD components of the STDP rule.

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Modelling constant potential amperometry for investigations of dopaminergic neurotransmission kinetics in vivo

Cited by 34 publications

References 27 publications

Real‐time decoding of dopamine concentration changes in the caudate–putamen during tonic and phasic firing

Real‐time decoding of dopamine concentration changes in the caudate–putamen during tonic and phasic firing

Electrochemical Probes for Detection and Analysis of Exocytosis and Vesicles

Spiking neurons, dopamine, and plasticity: Timing is everything, but concentration also matters

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