A parametric sensitivity study to improve the transcription quality of the nanofibrous bifurcated-tubular scaffold is performed to resolve the limited transcription quality issue in fabricating the scaffold based on hydrogel-assisted electrospinning (GelES). To characterize the transcription quality of the fabricated 3D nanofibrous bifurcated-tubular scaffold structure, two different measures of a coverage ratio (C R ) and a corner profile fidelity (F C ) are evaluated. Results of sensitivity analysis on C R , in which the design of experiments is based on the Taguchi method, revealed that the C R increases with decreasing tip-to-collector distance (TCD) and polycaprolactone concentration (C PCL ). Moreover, among the processing parameters, namely, TCD, C PCL , and applied voltage (V A ), the TCD is found to be the dominantly affecting processing parameter. Parametric study results show that F C increases with the branching angle (𝜽 B ). By combining the optimal processing condition and a maximum 𝜽 B of 120°, a leakage-free 3D nanofibrous bifurcated-tubular scaffold with a sharp carina is fabricated by the GelES.