Modelling glycogen storage and denitrification capability of microorganisms in enhanced biological phosphate removal processes

doi:10.1016/0273-1223(95)00177-o

Cited by 73 publications

(6 citation statements)

References 9 publications

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“…have been widely reported to be abundant in both lab-scale EBPR reactors (Mino et al 1995;Crocetti et al 2002;Kong et al 2002;Zeng et al 2003b) and full-scale EBPR plants (Crocetti et al 2002;Saunders et al 2003;Kong et al 2006). Surprisingly, in this study, Competibacter spp.…”

Section: Pre-fermentation Of Raw Wastewatercontrasting

confidence: 78%

A sequencing batch reactor system for high-level biological nitrogen and phosphorus removal from abattoir wastewater

et al. 2008

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A sequencing batch reactor (SBR) system is demonstrated to biologically remove nitrogen, phosphorus and chemical oxygen demand (COD) to very low levels from abattoir wastewater. Each 6 h cycle contained three anoxic/anaerobic and aerobic sub-cycles with wastewater fed at the beginning of each anoxic/anaerobic period. The step-feed strategy was applied to avoid high-level build-up of nitrate or nitrite during nitrification, and therefore to facilitate the creation of anaerobic conditions required for biological phosphorus removal. A high degree removal of total phosphorus (>98%), total nitrogen (>97%) and total COD (>95%) was consistently and reliably achieved after a 3-month start-up period. The concentrations of total phosphate and inorganic nitrogen in the effluent were consistently lower than 0.2 mg P l(-1) and 8 mg N l(-1), respectively. Fluorescence in situ hybridization revealed that the sludge was enriched in Accumulibacter spp. (20-40%), a known polyphosphate accumulating organism, whereas the known glycogen accumulating organisms were almost absent. The SBR received two streams of abattoir wastewater, namely the effluent from a full-scale anaerobic pond (75%) and the effluent from a lab-scale high-rate pre-fermentor (25%), both receiving raw abattoir wastewater as feed. The pond effluent contained approximately 250 mg N l(-1) total nitrogen and 40 mg P l(-1) of total phosphorus, but relatively low levels of soluble COD (around 500 mg l(-1)). The high-rate lab-scale pre-fermentor, operated at 37 degrees C and with a sludge retention time of 1 day, proved to be a cheap and effective method for providing supplementary volatile fatty acids allowing for high-degree of biological nutrient removal from abattoir wastewater.

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Section: Pre-fermentation Of Raw Wastewatercontrasting

confidence: 78%

A sequencing batch reactor system for high-level biological nitrogen and phosphorus removal from abattoir wastewater

et al. 2008

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“…Accumulibacter” PAO phenotype, except polyphosphate is not cycled, which is compensated by a reliance on glycogen as energy source under anaerobic conditions (Liu et al, 1994; Mino et al, 1995). As they compete with the PAO for resources without contributing to P removal, their proliferation is widely considered to be at the expense of EBPR efficiency (Seviour et al, 2003; Oehmen et al, 2007).…”

Section: Introductionmentioning

confidence: 99%

A Critical Assessment of the Microorganisms Proposed to be Important to Enhanced Biological Phosphorus Removal in Full-Scale Wastewater Treatment Systems

et al. 2017

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Understanding the microbiology of phosphorus (P) removal is considered essential to knowledge-based optimization of enhanced biological P removal (EBPR) systems. Biological P removal is achieved in these systems by promoting the growth of organisms collectively known as the polyphosphate accumulating organisms (PAOs). Also considered important to EBPR are the glycogen accumulating organisms (GAOs), which are theorized to compete with the PAOs for resources at the expense of P removal efficiency. Numerous studies have sought to identify the PAOs and their GAOs competitors, with several candidates proposed for each over the last few decades. The current study collectively assessed the abundance and diversity of all proposed PAOs and GAOs in 18 Danish full-scale wastewater treatment plants with well-working biological nutrient removal over a period of 9 years using 16S rRNA gene amplicon sequencing. The microbial community structure in all plants was relatively stable over time. Evidence for the role of the proposed PAOs and GAOs in EBPR varies and is critically assessed, in light of their calculated amplicon abundances, to indicate which of these are important in full-scale systems. Bacteria from the genus Tetrasphaera were the most abundant of the PAOs. The “Candidatus Accumulibacter” PAOs were in much lower abundance and appear to be biased by the amplicon-based method applied. The genera Dechloromonas, Microlunatus, and Tessaracoccus were identified as abundant putative PAO that require further research attention. Interestingly, the actinobacterial Micropruina and sbr-gs28 phylotypes were among the most abundant of the putative GAOs. Members of the genera Defluviicoccus, Propionivibrio, the family Competibacteraceae, and the spb280 group were also relatively abundant in some plants. Despite observed high abundances of GAOs (periodically exceeding 20% of the amplicon reads), P removal performance was maintained, indicating that these organisms were not outcompeting the PAOs in these EBPR systems. Phylogenetic diversity within each of the PAOs and GAOs genera was observed, which is consistent with reported metabolic diversity for these. Whether or not key traits can be assigned to sub-genus level clades requires further investigation.

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“…So far, an uncultured and unclassified genus belonging to the Beta-proteobacteria , named Candidatus Accumulibacter phosphatis (CAp), has been shown to be a dominating phosphorus accumulating organism (PAO) in laboratory-scale reactors (Hesselmann et al, 1999; Crocetti et al, 2000) as well as in full-scale BWWTPs (Zilles et al, 2002; Wong et al, 2005). Moreover, subsequent molecular investigations have identified glycogen accumulating organisms (GAOs; Mino et al, 1995) as major competitors of CAp in anaerobic/aerobic sludge cycling. This includes a novel group belonging to Gamma-proteobacteria named as Candidatus Competibacter phosphatis (Crocetti et al, 2002) and other groups belonging to Alpha-proteobacteria (Wong et al, 2004).…”

Section: The Current Microbiological and Biochemical Knowledge Of As-mentioning

confidence: 99%

A hundred years of activated sludge: time for a rethink

2014

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Biological wastewater treatment plants (BWWTPs) based on the activated sludge (AS) process have dramatically improved worldwide water sanitation despite increased urbanization and industrialization. However, current AS-based operations are considered economically and environmentally unsustainable. In this Perspective, we discuss our current understanding of microbial populations and their metabolic transformations in AS-based BWWTPs in view of developing more sustainable processes in the future. In particular, much has been learned over the course of the past 25 years about specialized microorganisms, which could be more comprehensively leveraged to recover energy and/or nutrients from wastewater streams. To achieve this, we propose a bottom-up design approach, focused around the concept of a “wastewater biorefinery column”, which would rely on the engineering of distinct ecological niches into a BWWTP in order to guarantee the targeted enrichment of specific organismal groups which in turn will allow the harvest of high-value resources from wastewater. This concept could be seen as a possible grand challenge to microbial ecologists and engineers alike at the centenary of the discovery of the AS process.

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Modelling glycogen storage and denitrification capability of microorganisms in enhanced biological phosphate removal processes

Cited by 73 publications

References 9 publications

A sequencing batch reactor system for high-level biological nitrogen and phosphorus removal from abattoir wastewater

A sequencing batch reactor system for high-level biological nitrogen and phosphorus removal from abattoir wastewater

A Critical Assessment of the Microorganisms Proposed to be Important to Enhanced Biological Phosphorus Removal in Full-Scale Wastewater Treatment Systems

A hundred years of activated sludge: time for a rethink

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