Modelling gradient elution in centrifugal partition chromatography

Buel, M. J. van; Wielen, Luuk A.M. van der; Luyben, K. Ch. A. M.

doi:10.1016/s0021-9673(97)00245-8

Cited by 28 publications

(13 citation statements)

References 8 publications

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“…Table lists the obtained values for the eight liquid systems. As often observed with different liquid systems [], the natural log value of the experimental partition coefficients was found to change linearly with the water content, % W , of the aqueous lower phase:

ln (K_{normalC}) = A % W + B

…”

Section: Resultsmentioning

confidence: 53%

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Modeling gradient elution in countercurrent chromatography: Efficient separation of tanshinones from Salvia miltiorrhizaBunge

Liang

et al. 2012

J of Separation Science

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Countercurrent chromatography (CCC) is a support-free liquid-liquid chromatography using centrifugal fields to hold the liquid stationary phase. CCC has been widely applied in the separation of various natural and synthetic components using a variety of biphasic liquid systems. The related hexane or heptane/ethyl acetate/methanol or ethanol/water biphasic liquid systems demonstrated their significance in CCC. Gradient is difficult in CCC since any composition change in one phase induces a composition change of the other phase to maintain phase equilibrium. This work provides a new insight into linear gradient elution in CCC that is feasible with some biphasic liquid systems such as selected compositions of the hexane/ethyl acetate/ethanol/water systems. The equations modeling solute motion inside the CCC column are proposed. Particular compositions of the liquid system, namely the hexane/ethyl acetate/ethanol/water 8:2:E:W compositions with E + W = 10, were studied from W = 1 to 9. They showed moderate changes in the upper organic phase compositions. The model is tested with the separation of tanshinones from the rhizome of Salvia miltiorrhiza Bunge. Different linear solvent gradient profiles were experimentally performed between 8:2:5:5 and 8:2:3:7 compositions and the results were evaluated using the proposed model. Five tanshinones including dihydrotanshinone I, cryptotanshinone, tanshinone I, 1,2-dihydrotanshinquinone, and tanshinone IIA have been successfully separated (>95% purities) using a gradient profile optimized by the developed model. The gradient model can be used only with biphasic liquid systems in which one phase shows minimum composition changes when the other phase composition changes notably. This case is not the general case for biphasic liquid systems but can be applied with specific compositions of the quaternary hexane or heptane/ethyl acetate/methanol or ethanol/water most useful CCC liquid systems.

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ln (K_{normalC}) = A % W + B

…”

Section: Resultsmentioning

confidence: 53%

“…In HPLC, the silica bonded stationary phase shows little changes during the mobile phase gradient composition change. This is not the case in CCC explaining why so few theoretical developments were proposed in CCC [].…”

Section: Introductionmentioning

confidence: 99%

Modeling gradient elution in countercurrent chromatography: Efficient separation of tanshinones from Salvia miltiorrhizaBunge

Liang

et al. 2012

J of Separation Science

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“…Gradient elution in CPC is an efficient approach that enables the fractionation of complex crude mixtures in less time than classical isocratic elution procedures [18][19][20][21]. However, its applications in solid support free liquid-liquid chromatography remain scarce, mainly because the modifications of the stationary phase composition during the mobile phase gradient are poorly understood and thus poorly controlled.…”

Section: Separation Of Sophorolipids By Cpcmentioning

confidence: 99%

Gradient elution method in centrifugal partition chromatography for the separation of a complex sophorolipid mixture obtained from Candida bombicola yeasts

Kotland

Hadef

Renault

et al. 2013

J of Separation Science

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Sophorolipids represent an important class of natural surfactants with a variety of environmental, cosmetic, and pharmaceutical applications. Despite their promising physicochemical and biological properties, the use of sophorolipids is hampered by the lack of information regarding their individual structure-activity relationships. The major difficulty in isolating pure sophorolipids arises from the high complexity of crude fermentation media composition and from their strong structural similarities. In this work, a centrifugal partition chromatography method was developed in an original gradient elution mode for the separation of sophorolipids produced by the yeast Candida bombicola. Experiments were realized by using three sets of solvent systems composed of n-heptane, ethyl acetate, n-butanol, methanol, and water in different proportions. The separation was performed at 5 mL/min in the ascending mode by increasing progressively the polarity of the organic mobile phase. In these conditions, more than 80% of the sophorolipids present in the initial crude fermentation extract were eluted successively from the most hydrophobic lactone forms to the most hydrophilic acid forms. The structures of the isolated sophorolipids were further elucidated by HPLC and NMR analyses.

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“…It had already been pointed out that some properties of proteins can show extremely high resolution in purification operations (e.g., charge and affinity), whereas others show much less resolution (e.g., molecular weight). The main thrust was focused on the development of novel techniques such as cross-flow electrofiltration, 3,4 reversed micelles, 5 -7 centrifugal partitioning, 8 membrane chromatography, 9,10 new affinity ligands, 11,12 aqueous two-phase partitioning 13 -16 and continuous processing (e.g., CARE). 17,18 It was also stressed that the development of new and efficient separation processes had to be based on more efficiently exploiting differences in the actual physicochemical properties of the protein product (surface charge/titration curve, surface hydrophobicity, molecular weight, biospecificity, pI, stability) compared to those of contaminant components in the crude broth.…”

Section: Introductionmentioning

confidence: 99%

Challenges and trends in bioseparations

Asenjo

Andrews

2008

J of Chemical Tech & Biotech

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In this paper the issues and challenges presented 15 years ago for performing efficient separation processes for recombinant proteins are revised and discussed. Competitive advantage in production was seen as not only dependent on innovations in molecular biology and other areas of basic biological sciences but also on innovation of separations and downstream processes. The trend to develop techniques that exploit fundamental physicochemical principles more efficiently was emphasized, including analysis of the physicochemical properties of proteins and its relation to efficiency in bioseparation. 15 years ago the main thrust was also focused on the development of novel techniques. Clearly the challenges faced today, where highly optimized and efficient production processes exist, are dramatically different. The use of mathematical models for optimizing chromatographic separations and simplifying validation of such operations is extremely advantageous. Their use constitutes an example of how the challenges that bioseparations are facing and will be facing within the next few years can be met. Such models should be extended to a larger number of proteins, chromatographic procedures and experimental conditions

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Modelling gradient elution in centrifugal partition chromatography

Cited by 28 publications

References 8 publications

Modeling gradient elution in countercurrent chromatography: Efficient separation of tanshinones from Salvia miltiorrhizaBunge

Modeling gradient elution in countercurrent chromatography: Efficient separation of tanshinones from Salvia miltiorrhizaBunge

Gradient elution method in centrifugal partition chromatography for the separation of a complex sophorolipid mixture obtained from Candida bombicola yeasts

Challenges and trends in bioseparations

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