Modelling migration behavior of peptide hormones in capillary electrophoresis-electrospray mass spectrometry

Benavente, Fernando; Balaguer, Elvira; Barbosa, J.; Sanz‐Nebot, Victoria

doi:10.1016/j.chroma.2006.03.049

Cited by 39 publications

(75 citation statements)

References 23 publications

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“…Separation electrolytes composed of ammonium formate and acetate have been successfully used in many CE-ESI-MS experiments, as the volatility of the separation electrolyte components is mandatory in order to generate an efficient spray, improve detectability and prevent formation of salt deposits in the interface with the mass spectrometer [8,9,34]. In general, better sensitivity has been obtained at low pH values.…”

Section: Ce-uvmentioning

confidence: 98%

“…For CE-ESI-MS experiments, the PrinCE system was coupled to an LCQ-DECA XP Plus IT mass spectrometer (Thermo Electron, San José, CA, USA) using a custommade ESI sheath-flow interface without nebulizer gas [8,9,33,34]. The ESI electrode consisted of a 15 cm6410 mm id61000 mm od stainless-steel tube.…”

Section: Ce-esi-msmentioning

confidence: 99%

“…The test mixture was also used to optimize the sheathliquid composition. This optimization was made by measuring the peak area of each reference compound in the extracted ion electropherogram (XIE) obtained from the most intense peak corresponding to a molecular ion in their mass spectrum [34]. The CE was controlled from the front panel of the instrument.…”

Section: Ce-esi-msmentioning

confidence: 99%

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Metabolite profiling of human urine by CE-ESI-MS using separation electrolytes at low pH

et al. 2006

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We investigated the potential of CE coupled to electrospray MS (CE-ESI-MS) in metabolite profiling of human urine without any sample prefractionation step. A heterogeneous mixture of biologically relevant compounds covering a broad range of physicochemical properties was used to optimize separation conditions in fused-silica capillaries. A running electrolyte containing 50 mM of acetic acid and 50 mM of formic acid at pH 2.5 was used for the CE separations. A sheath-flow electrospray interface was employed for CE-ESI-MS analysis. Sheath liquids containing 80:20 v/v methanol/water with 0.1% v/v of acetic acid or 60:40 v/v isopropanol/water with 0.5% v/v of ammonia were selected for optimum detection in positive and negative ESI modes, respectively. Reproducibility and sensitivity were studied, and strategies for identification of the separated urinary compounds are suggested. We report major advantages and disadvantages of CE-ESI-MS for metabolite profiling of human body fluids. This work may be regarded as a first step in the use of CE-ESI-MS for reliable differential analysis of body fluids from healthy and diseased individuals.

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Section: Ce-uvmentioning

confidence: 98%

Section: Ce-esi-msmentioning

confidence: 99%

Section: Ce-esi-msmentioning

confidence: 99%

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Metabolite profiling of human urine by CE-ESI-MS using separation electrolytes at low pH

et al. 2006

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“…Therefore, the discovery of new biomarkers that could help to the diagnosis process is of great interest. Several works have been carried out with this aim [45,60,70,74,75,98,99]. Thus, Kaiser et al [74] were able to establish a polypeptide pattern in human fluid samples comprising more than 1000 polypeptides within 45-60 min analysis times by means of a CE-ESI-TOF instrument.…”

Section: Ce-ms Using Bare Fused-silica Capillariesmentioning

confidence: 97%

“…In this regard, an important limitation arises from the frequent generation of unexpected peptidic fragments during the enzymatic digestion of any protein. To solve this problem, the use of theoretical models to simulate peptide behavior in CE can be very useful [47,48,66,70]. Our group has developed a theoretical model to effectively relate the peptide sequence to their electrophoretic behavior [47].…”

Section: Ce-ms Using Bare Fused-silica Capillariesmentioning

confidence: 99%

Capillary electrophoresis‐electrospray‐mass spectrometry in peptide analysis and peptidomics

2008

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In the present work, an exhaustive review of the main developments and applications of CE-MS for peptide analysis is given. This review includes the use of different CE separation modes, MS analyzers, capillary coatings, preconcentration techniques, on-chip applications as well as other different multidimensional strategies for peptide analysis. Key applications are critically discussed and relevant works published from January 2000 to May 2007 are summarized including information concerning the type of sample, CE-MS parameters as well as some figures of merit of the different CE-MS procedures developed for peptide analysis and peptidomics.

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Metabolomics: High‐Resolution Tools Offer to Follow Bacterial Growth on a Molecular Level

Lucio

Fekete

Frommberger³

et al. 2011

Handbook of Molecular Microbial Ecology I

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Modelling migration behavior of peptide hormones in capillary electrophoresis-electrospray mass spectrometry

Cited by 39 publications

References 23 publications

Metabolite profiling of human urine by CE-ESI-MS using separation electrolytes at low pH

Metabolite profiling of human urine by CE-ESI-MS using separation electrolytes at low pH

Capillary electrophoresis‐electrospray‐mass spectrometry in peptide analysis and peptidomics

Metabolomics: High‐Resolution Tools Offer to Follow Bacterial Growth on a Molecular Level

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