We numerically simulate the hydrodynamic interaction of aerosol particles due to the acoustic wake effect under the Oseen flow condition. Attraction is found for two nearby particles with an orientation angle of 0 to 50° with respect to the acoustic field, and weak repulsion is found outside this range. Good agreement is obtained between the numerical results and experiments in the literature. We study the influence of particle size, sound wave frequency and the particle separation. The result shows that the acoustic wake effect plays a significant role in acoustic agglomeration. It could be either the major agglomeration mechanism of monodisperse aerosols or the major refill mechanism for polydisperse aerosols to supplement orthokinetic interaction. Acoustic agglomeration is a process in which intense sound waves produce relative motions and collisions among aerosol particles. It can significantly shift the particle size distribution of an aerosol from smaller to larger sizes in a short time of the order of 1 s. Acoustic agglomeration has potential use in air pollution control as an aerosol preconditioning process to enhance the performance of conventional particle filtering devices, which are inefficient for retaining particles smaller than 2.5 m [1]. Acoustic agglomeration is governed by complex interaction mechanisms. The most significant ones are orthokinetic and hydrodynamic interactions [2]. Orthokinetic interaction is the most obvious mechanism. Particles with different sizes are entrained differently into the oscillating motion of the medium because of the differences in particle inertia. The relative motion leads to the approach and collision between particles. In one acoustic cycle, each larger particle sweeps a certain volume by its motion relative to smaller particles, and collects all the smaller particles in it. This volume is defined as the agglomeration volume. The mechanism of orthokinetic interaction was well summarized by Mednikov [3] and improved by other researchers [4][5][6]. Nevertheless, this mechanism cannot explain agglomeration of monodisperse aerosols or the way in which the agglomeration volume is refilled once it is emptied after one cycle.Hydrodynamic mechanisms are those which produce particle interactions through the surrounding medium because of hydrodynamic forces and the asymmetry of the flow field around the particle [1]. These mechanisms are generally considered to be the main acoustic agglomeration mechanisms for monodisperse aerosols because they are also active for same-sized particles [7]. Hydrodynamic mechanisms mainly include mutual radiation pressure and the acoustic wake effect [5,6,8,9]. The mutual radiation pressure is the effect due to the nonlinear interactions produced between the particle scattering wave and the incident field. However, so far, there is still no experiment to confirm this effect as a cause of agglomeration, and theoretical studies show that the mutual radiation pressure is much less important than the acoustic wake effect in acoustic agglome...