Modelling of Lemna minor L. growth as influenced by nutrient supply, supplemental light, CO2 and harvest intervals for a continuous indoor cultivation

Schmidt, Karl-Michael; Goldbach, Heiner E.

doi:10.1016/j.heliyon.2022.e12194

Cited by 2 publications

(2 citation statements)

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“…Furthermore, even some of the bacterial strain known to be PGPB could promote growth penalties in case of nutrient deficiency [ 43 ]. Moreover, the competition with duckweeds and algae ( Chlorella sp., Chlamydomonas sp ., …), in the first phase, is a severe threat for long-term cultivation [ 44 – 46 ]. These algae could reduce N, P, Fe and Mn concentrations of the medium drastically and increase the pH beyond 10 [ 45 ].…”

Section: Discussionmentioning

confidence: 99%

Characterization of the physico-chemical properties of the natural habitat and in vitro culture effects on the biochemistry, proliferation and morphology of Lemna minuta

et al. 2023

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In this study, the ecological conditions of the natural habitat of Lemna minuta Kunth in Morocco were investigated, and the impact of five synthetic growth media (Murashige-Skoog (MS), Schenk-Hildebrand (SH), Hoagland medium (HM), 10X Algal Assay Procedure (AAP), and Swedish Standard Institute medium (SIS)) on the morphophysiological and biochemical parameters was analysed. The morphophysiological parameters included root length, frond surface area, and fresh weight, while the biochemical parameters included photosynthetic pigments, carbohydrates, and protein content. The study was conducted in vitro in two phases: an uncontrolled aeration system (Phase I) and a controlled aeration system (Phase II).The results showed that the pH, conductivity, salinity, and ammonium levels in the natural habitat were within the optimal range for duckweed growth. The measured orthophosphate concentrations were higher compared to previous observations, while the recorded chemical oxygen demand values were low. The study also revealed a significant effect of the culture medium composition on the morphophysiological and biochemical parameters of the duckweed. The fresh weight biomass, relative growth rate in fronds, relative growth rate in surface area, root length, protein content, carbohydrates, chlorophyll (a), chlorophyll (b), total chlorophyll, carotenoids, and the chlorophyll (a/b) ratio were all affected by the culture medium.The most accurate regression models described the growth index GI(F) based on time and in vitro culture conditions in both phases. In Phase I, the best models for MS, SIS, AAP, and SH media were linear, weighted quadratic, cubic, and weighted cubic, respectively. In Phase II, the best models for all growth media were linear. The time coefficients (in days) for Phase II were 0.321, 0.547, 1.232, 1.470, and 0.306 for AAP, HM, MS, SH, and SIS, respectively.Comparing the morphophysiological and biochemical parameters of fronds from different media and analysing the regression model results showed that the SH and MS media were the best among the tested media for the in vitro culture of L. minuta in controlled aeration conditions. However, further research is needed to develop new synthetic media that best promote the growth and maintenance of this duckweed in long-term culture.

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Section: Discussionmentioning

confidence: 99%

Characterization of the physico-chemical properties of the natural habitat and in vitro culture effects on the biochemistry, proliferation and morphology of Lemna minuta

et al. 2023

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“…For generation of aerosol, liquid BG11 medium was pumped in a vessel with an ultrasonic transducer (NW‐80E‐01; TDK Europe), which was already described by Strieth et al (2017) and is based on a patent by Schmidt and Just (WO2007068467A1, 2006). The transducer vessel used here, had one additional connection over the ultrasonic transducer, to ensure a constant medium level over the transducer.…”

Section: Methodsmentioning

confidence: 99%

A new easy method for determination of surface adhesion of phototrophic biofilms

Stiefelmaier,

Strieth,

Schaefer

et al. 2023

Biotech & Bioengineering

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Terrestrial cyanobacteria grow as phototrophic biofilms and offer a wide spectrum of interesting products. For cultivation of phototrophic biofilms different reactor concepts have been developed in the last years. One of the main influencing factors is the surface material and the adhesion strength of the chosen production strain. In this work a flow chamber was developed, in which, in combination with optical coherence tomography and computational fluid dynamics simulation, an easy analysis of adhesion forces between different biofilms and varied surface materials is possible. Hereby, differences between two cyanobacteria strains and two surface materials were shown. With longer cultivation time of biofilms adhesion increased in all experiments. Additionally, the content of extracellular polymeric substances was analyzed and its role in surface adhesion was evaluated. To test the comparability of obtained results from the flow chamber with other methods, analogous experiments were conducted with a rotational rheometer, which proved to be successful. Thus, with the presented flow chamber an easy to implement method for analysis of biofilm adhesion was developed, which can be used in future research for determination of suitable combinations of microorganisms with cultivation surfaces on lab scale in advance of larger processes.

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Modelling of Lemna minor L. growth as influenced by nutrient supply, supplemental light, CO2 and harvest intervals for a continuous indoor cultivation

Cited by 2 publications

References 46 publications

Characterization of the physico-chemical properties of the natural habitat and in vitro culture effects on the biochemistry, proliferation and morphology of Lemna minuta

Characterization of the physico-chemical properties of the natural habitat and in vitro culture effects on the biochemistry, proliferation and morphology of Lemna minuta

A new easy method for determination of surface adhesion of phototrophic biofilms

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