Modelling of Reactive Black 5 decolourization in the presence of heavy metals by the newly isolated Pseudomonas aeruginosa strain Gb30

Abstract

Aim The effect of heavy metals presence on the decolourization of Reactive Black 5 by Pseudomonas aeruginosa was evaluated. Methods and Results In the current study, a newly isolated strain identified as P. aeruginosa strain Gb 30 was selected for its ability to remove high concentration of Reactive Black 5 and resistance to several heavy metals (Cu2+>Zn2+>Cd2+>Cr6+). Strain Gb30 was used to assess the effect of heavy metals presence on RB5 decolourization. The strain growth exhibited different responses at a fixed concentration of EC50 (10 h) for each heavy metal. The addition of Zn2+ and Cd2+ had no effect on decolourization yield after 24 h of incubation, whereas Cr6+ and Cu2+ ions reduced decolourization up to 17%. In order to understand the relationship between heavy metals contamination and decolourization, experimental data relating the initial decolourization rate of RB5 to the concentrations of single and associated heavy metals were fitted to three different inhibition kinetic models. Conclusions In this study, we showed that P. aeruginosa strain Gb30 could be used for dye removal even at high concentrations of heavy metals. The developed models could provide basic information that may help for the best management of the bacteria‐mediated decolourization process at the industrial scale. Significance and Impact of the Study This study opens new directions for the management of textile industry wastewaters containing dyes and heavy metals using bioaugmentation by P. aeruginosa strain Gb30.

Microbial decolorization of Reactive Black 5 dye by Bacillus albus DD1 isolated from textile water effluent: kinetic, thermodynamics & decolorization mechanism

“…Maximum color removal, 92.3 ± 3.4%, was achieved at 1% concentration of yeast extract within 72 h, thus lowering the overall time required for decolourization. Microorganisms for reactive dyes decolourization are greatly affected by their growth determined by the availability of nutrients ( Louati et al., 2019 ). Nitrogen (yeast extract) is an important nutrient as a primary contributor to color degradation.…”

Modelling the Effect of Copper on the Growth Rate of Enterobacter sp. strain Neni-13 on SDS

“…In the study on the inhibitive effects of heavy metals on Reactive Black 5 decolourization by Pseudomonas aeruginosa strain Gb30, the best model modelling the inhibitory effect of heavy metals on the decolourization rate was Han-Levenspiel with Ccrit, µmax and values of 3496 mg/L (50 mM), 2.013 h -1 and 1.193, respectively, for zinc and 280 mg/L (2.491 mM), 1.991 h -1 and 0.882, respectively, for cadmium [19]. However, despite the fact that heavy metals and organic pollutants are both ubiquitously present in polluted waters, the use of metal inhibition models is underrepresented in the literature.…”

Simultaneous cleanup of Reactive Black 5 and cadmium by a desert soil bacterium

“…Pseudomonas aeruginosa strain Gb 30 (KY655217.1) previously isolated from desert soil and characterized and identified in our laboratory, was selected for this work (Louati et al, 2019).…”