Modelling the Radiolysis of Silver Nitrate Solutions in presence of Bromide Ions in Liquid-Phase Transmission Electron Microscopy

Taherkhani, Mehran; Fritsch, Birk; Jank, Michael P. M.; Spiecker, Erdmann; Hutzler, Andreas

doi:10.1017/s1431927621013519

Cited by 1 publication

(1 citation statement)

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“…Heterogeneous nucleation could occur at the liquid/graphene interface even though these interface reactions are expected to be smaller at low atomic number materials 37 . Second, the cascade of reactions which occur when the beam interacts with the aqueous buffer solution will also influence the crystallization 37,51 . Besides, multiple ionic species are already present in the liquid next to the sodium and chloride ions.…”

Section: Crystallization In Glcsmentioning

confidence: 99%

A Cryo-/Liquid Phase Correlative Light Electron Microscopy Workflow to Visualize Crystallization Processes in Graphene Liquid Cells

Rutten

Beer

Roverts

et al. 2023

Preprint

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Liquid phase electron microscopy has emerged as a powerful technique for in-situ observation of material formation in liquid. However, the interaction of electrons with an aqueous environment leads to the decomposition of water molecules into multiple radicals (radiolysis), which can affect the formation process. Graphene's ultra-conductive properties have made it an efficient way to mitigate this problem, as it acts as an electron scavenger when used as a window material. However, finding accessible and reliable protocols for the formation of graphene liquid cell (GLC) has been an enormous challenge. Even though loop-assisted transfer has recently been demonstrated as an effective method, the liquid cells are randomly formed and the process of interest is initiated when the GLCs are sealed. This means that the process cannot be imaged at early timepoints, because searching for their location is a time-consuming effort during which the sample is exposed to electrons. Here we report a novel cryogenic/liquid phase correlative light/electron microscopy workflow that addresses some of the limitations of the graphene liquid cells, combining the advantages of fluorescence and electron microscopy. Fluorescence microscopy (FM) is used to locate the GLCs without exposure to electrons and electron microscopy to capture dynamics with nanoscale resolution. This workflow allows imaging to be initiated at a predetermined space and time by vitrifying and thawing at the moment of interest. We demonstrate the workflow by observing non-classical crystallization pathways, but also highlight its potential application in biological systems.

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Section: Crystallization In Glcsmentioning

confidence: 99%