Modelling the stress inducing biphasic growth and pediocin production by Pediococcus acidilactici NRRL B-5627 in re-alkalized fed-batch cultures

Guerra, Nelson Pérez; Bernárdez, Paula Fajardo; Pastrana, Lorenzo

doi:10.1016/j.bej.2008.02.001

Cited by 16 publications

(24 citation statements)

References 25 publications

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“…As observed by previous researchers [9,11] similar to lactic acid, pediocin production is also partly related to cell growth and partly independent of growth. Thus, Luedeking-Piret model [11] is applicable for the rate of pediocin production (q P ).…”

Section: Pediocin Productionsupporting

confidence: 59%

“…Therefore, the exact intracellular reactions, as considered in structured models [7], are not incorporated, and cells are assumed to be constituted of uniform biomass without any demarcation as described in classical Monod type [8] growth models. As evident from experimental observation of the present study as well as from literature review [9], growth of P. acidilactici culture depends on two substrates, namely, protein and glucose. In addition, cell growth is inhibited by the production of lactic acid.…”

Section: Growth Kineticsmentioning

confidence: 69%

“…In addition, cell growth is inhibited by the production of lactic acid. From the literature survey, it appears that different types of growth kinetics have so far been attempted [10] to incorporate the effects of concentration of glucose, protein, and lactic acid on the growth of P. acidilactici strain [9]. For the simplicity of the model, a multiplicative type dual substrate (glucose and protein) model along with reciprocal correlation term for lactic acid inhibition has been attempted.…”

Section: Growth Kineticsmentioning

confidence: 99%

“…As described, the value of K iLA has been determined by plotting (1/ω−1) against initial lactic acid concentration using Equation (9). The values of μm, K S 1 and K S 2 are used as obtained previously.…”

Section: Estimation Of K Ilamentioning

confidence: 99%

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Study the Growth Kinetics of Pediococcus Acidilactici With Estimation of Kinetic Parameters and Applied in Large Scale Pediocin Production

De¹

2016

Asian J Pharm Clin Res

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Objectives: The aim of the study was to determine the growth kinetics of Pediococcus acidilactici using a mathematical model for large scale pediocin production.Methods: Growth kinetics of P. acidilactici has been studied for pediocin production in small scale batch fermenter (Erlenmeyer flask) using meat processing waste medium. The experiments have been conducted with varying the concentrations of glucose, protein, and lactic acid. A mathematical model has been developed to describe growth rate, products (pediocin and lactic acid) formation rate, and substrates (glucose and protein) utilization rate. Monod model for dual substrates (glucose and protein) has been used with considering lactic acid inhibition. Luedeking-Piret model has been introduced to describe the production of pediocin and lactic acid. Results:The values of kinetic parameters have been determined using experimental data and model equations. The model prediction has been compared satisfactorily with the experimental data for the validation of the model. Conclusions:The developed model was satisfactorily validated to scale up the production of pediocin.

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Section: Pediocin Productionsupporting

confidence: 59%

Section: Growth Kineticsmentioning

confidence: 69%

Section: Growth Kineticsmentioning

confidence: 99%

Section: Estimation Of K Ilamentioning

confidence: 99%

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Study the Growth Kinetics of Pediococcus Acidilactici With Estimation of Kinetic Parameters and Applied in Large Scale Pediocin Production

De¹

2016

Asian J Pharm Clin Res

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“…Bacteriocin production from LAB has been mostly studied in batch cultures using synthetic media . A number of studies, however, have demonstrated that production can be improved in fed‐batch systems . In most reported cases on batch and fed‐batch systems, bacteriocin production by LAB is a growth‐associated process .…”

Section: Introductionmentioning

confidence: 99%

Chemostat production of pediocin SM‐1 by Pediococcus pentosaceus Mees 1934

Papagianni

Sergelidis

2015

Biotechnology Progress

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Production of the bacteriocin pediocin SM-1 by Pediococcus pentosaceus Mees 1934 was investigated in pH-controlled batch and chemostat cultures using a complex medium containing glucose, sucrose or fructose. In chemostat cultures operated at 150 rpm, 30°C, 60% dissolved oxygen tension, pH 6.5, and D = 0.148 h(-1) , the pediocin titer reached 185 AU/mL representing an increase of 32% compared with batch cultures in which glucose was used as the carbon source. Pediocin biosynthesis was markedly affected by the growth rate of the producer microorganism. For all carbon sources tested, pediocin production appeared to take place only at dilution rates lower than μmax . However, only glucose supported production at the very low dilution rate of 0.05 h(-1) indicating a direct regulation of pediocin biosynthesis by the carbon source. Glucose supported higher biomass productivity and higher pediocin titers and yields compared with the other sugars used.

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Nisin production in realkalized fed-batch cultures in whey with feeding with lactose- or glucose-containing substrates

Malvido

González

Guerra

2016

Appl Microbiol Biotechnol

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Nisin production by Lactococcus lactis CECT 539 was followed in batch cultures in whey supplemented with different concentrations of glucose and in two realkalized fed-batch fermentations in unsupplemented whey, which were fed, respectively, with concentrated solutions of lactose and glucose. In the batch fermentations, supplementation of whey with glucose inhibited both the growth and bacteriocin production. However, fed-batch cultures were characterized with high productions of biomass (1.34 and 1.51 g l(-1)) and nisin (50.6 and 60.3 BU ml(-1)) in comparison to the batch fermentations in unsupplemented whey (0.48 g l(-1) and 22.5 BU ml(-1)) and MRS broth (1.59 g l(-1) and 50.0 BU ml(-1)). In the two realkalized fed-batch fermentations, the increase in bacteriocin production parallels both the biomass production and pH drop generated in each realkalization and feeding cycle, suggesting that nisin was synthesized as a pH-dependent primary metabolite. A shift from homolactic to heterolactic fermentation was observed at the 108 h of incubation, and other metabolites (acetic acid and butane-2,3-diol) in addition to lactic acid accumulated in the medium. On the other hand, the feeding with glucose improved the efficiencies in glucose, nitrogen, and phosphorus consumption as compared to the batch cultures. The realkalized fed-batch fermentations showed to be an effective strategy to enhance nisin production in whey by using an appropriate feeding strategy to avoid the substrate inhibition.

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Modelling the stress inducing biphasic growth and pediocin production by Pediococcus acidilactici NRRL B-5627 in re-alkalized fed-batch cultures

Cited by 16 publications

References 25 publications

Study the Growth Kinetics of Pediococcus Acidilactici With Estimation of Kinetic Parameters and Applied in Large Scale Pediocin Production

Study the Growth Kinetics of Pediococcus Acidilactici With Estimation of Kinetic Parameters and Applied in Large Scale Pediocin Production

Chemostat production of pediocin SM‐1 by Pediococcus pentosaceus Mees 1934

Nisin production in realkalized fed-batch cultures in whey with feeding with lactose- or glucose-containing substrates

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