In this paper we classified the sex of 39 Iberian Water Rails Rallus aquaticus using external morphometric measurements and genetic analysis. Logistic regression and classification tree model techniques (CART) were used to test whether simple morphometric measurements alone could classify sex correctly. For most of these measurements the overlap between sexes was too great to be of value. Bill length was the most relevant variable according to all the statistical analyses for the population under study. The applied combination of statistical techniques on biometric and genetic data correctly classify 80% of individuals. However, in view of the apparent variability in morphometric characteristics between populations, morphometric techniques to sex individuals from other populations should be validated using other criteria.
* Correspondence authorEmail: benito.fuertes@unileon.esThe Water Rail Rallus aquaticus is a polytypic, medium-sized bird (102-128 g) with a discontinuous trans-Palearctic distribution (Voous 1960, Cramp & Simmons 1980. In Europe, the subspecies aquaticus is widespread (Del Hoyo et al 1996) but accounts for less than half of its global breeding population of 140,000-360,000 pairs (BirdLife International 2004). Therefore, it is considered 'Not Threatened'. However, during recent decades it has suffered a continuous decrease in abundance, in common with other species of rails (Collar et al 1994, BirdLife International 2004, due to the disappearance and degradation of wetlands (Jenkins et al 1995, De Kroon 2004. The subspecies aquaticus, which includes Iberian birds, is mainly resident in the west and south of the range, but is partially migratory in the north and east (Cramp & Simmons 1980, De Kroon 1984a. Methods for determining the sex of Water Rails have scarcely been studied, in spite of the relative ease of capture of the species (Zembal & Massey 1983, De Kroon 1984b, Kearns et al 1998, Fuertes et al 2002. Biometric differences between the sexes have been described for European Water Rails, with males being significantly larger than females (Flegg & Glue 1973, Cramp & Simmons 1980, Becker 1990, 1995, De Kroon 2000. However, to our knowledge, there is no specific published information on morphometric parameters for sexing Iberian birds (Cramp & Simmons 1980, Baker 1993, even though sex ratio determination is fundamental for understanding both behaviour and population structure and dynamics, and for the design of management strategies and conservation plans (Jones et al 1995, Millar et al 1997, McGregor & Peake 1998.We study a Water Rail population in León province (NW of the Iberian Peninsula), which is strategically positioned at the boundary between the Mediterranean and Euro-Siberian biogeographical zones. Due to the lack of knowledge of this population, and since the use of biometric data from Northern and Central Europe to sex Iberian birds is not recommended (Campos et al 2005, Zuberogoitia et al 2005, we have employed DNA analyses as an alternative and more accurate method for cla...