Kerr frequency combs from microresonators are now extensively investigated as a potentially portable technology for a variety of applications. Most studies employ anomalous dispersion microresonators that support modulational instability for comb initiation, and mode-locking transitions resulting in coherent bright soliton-like pulse generation have been reported. However, some experiments show comb generation in normal dispersion microresonators; simulations suggest the formation of dark pulse temporal profiles. Excitation of dark pulse solutions is difficult due to the lack of modulational instability in the effective blue-detuned pumping region; an excitation pathway has been demonstrated neither in experiment nor in simulation. Here we report experiments in which dark pulse combs are formed by mode-interaction-aided excitation; for the first time, a mode-locking transition is observed in the normal dispersion regime. The excitation pathway proposed is also supported by simulations.Microresonator-based optical frequency combs, also termed Kerr combs, are generated through conversion of a single pump frequency to a broadband frequency comb inside a high-quality-factor (Q) microresonator via the third-order Kerr nonlinearity [1]- [10]. The advantages of Kerr combs include very compact size, high repetition rate, and capability of generating ultra-broad combs.The dynamics of Kerr comb generation have attracted intense investigations since the first demonstration of the method [11]-[28]. It has been found that Kerr combs are not always coherent [11]-[12] and may be characterized by high intensity noise [13]-[14]; furthermore, lack of coherence and high intensity noise are generally correlated. Experiments have revealed transitions from low coherence, high noise states to highly coherent mode-locked states accompanied by a sudden drop in the comb noise [14]- [18]. It has been found in simulations and experiments that the mode locking of broadband Kerr combs is usually related to soliton formation in the cavity [15], [17]-[28]. These dissipative cavity solitons are localized structures stabilized by a balance between Kerr nonlinearity and dispersion. In time domain they exist as bright or dark pulses, depending on whether the cavity dispersion is anomalous or normal, respectively. Bright microresonator solitons in the anomalous dispersion region have been observed in experiments and well studied through simulations [15], [17]-[27]. Reference [17] reported a method of tuning the pump laser frequency to an effectively red-detuned regime (pump laser wavelength longer than resonant wavelength) which is typically difficult to achieve due to thermal instability [29]. Mode-locking transitions yielding bright solitons were observed after passage through a broadband chaotic state [17]. In contrast, although dark solitons have been predicted in normal dispersion microresonators in 2 / 32 2 / 32 theory and simulation [27]-[28], investigating dark solitons experimentally is extremely difficult and no time-domain char...