Modelos animais para avaliação auditiva: revisão de literatura

Reis, Aléxia dos; Dalmolin, Suelen Pizzolatto; Dallegrave, Eliane

doi:10.1590/1982-021620171932117

Cited by 16 publications

(6 citation statements)

References 28 publications

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“…Guinea pigs display a great overlap with the audible frequency range in humans and their cochlea contains three and a half turns. This extra turn in guinea pigs can be useful in research using drugs that influence the cochlea (Reis et al, 2017 ; Naert et al, 2019 ). Other species that are frequently used for hearing research are rats, miniature pigs and non-human primates (NHPs).…”

Section: Discussionmentioning

confidence: 99%

“…Other species that are frequently used for hearing research are rats, miniature pigs and non-human primates (NHPs). The cochlea of rats measures two and a half turn and they often suffer from otitis media as their tympanic membrane does not completely seal the external auditory canal and their Eustachian tube is horizontally orientated (Reis et al, 2017 ). Miniature pigs are also an important animal model when considering ontological research.…”

Section: Discussionmentioning

confidence: 99%

“…Furthermore, pig deafness models have more similarities with humans in both genetics and disease phenotypes compared to rodents (Ji et al, 2019 ). Finally, NHPs could also prove to be useful in hearing research as there are many similarities with humans, not only anatomical but also in terms of progressive hearing damage and many physiological processes and phenotypes associated with human disorders display high similarity to NHP models (Reis et al, 2017 ; Burton et al, 2019 ).…”

Section: Discussionmentioning

confidence: 99%

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Transduction Efficiency and Immunogenicity of Viral Vectors for Cochlear Gene Therapy: A Systematic Review of Preclinical Animal Studies

Verdoodt

Peeleman

Camp

et al. 2021

Front. Cell. Neurosci.

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Background: Hearing impairment is the most frequent sensory deficit, affecting 466 million people worldwide and has been listed by the World Health Organization (WHO) as one of the priority diseases for research into therapeutic interventions to address public health needs. Inner ear gene therapy is a promising approach to restore sensorineural hearing loss, for which several gene therapy applications have been studied and reported in preclinical animal studies.Objective: To perform a systematic review on preclinical studies reporting cochlear gene therapy, with a specific focus on transduction efficiency.Methods: An initial PubMed search was performed on April 1st 2021 using the PRISMA methodology. Preclinical in vivo studies reporting primary data regarding transduction efficiency of gene therapy targeting the inner ear were included in this report.Results: Thirty-six studies were included in this review. Transduction of various cell types in the inner ear can be achieved, according to the viral vector used. However, there is significant variability in the applied vector delivery systems, including promoter, viral vector titer, etc.Conclusion: Although gene therapy presents a promising approach to treat sensorineural hearing loss in preclinical studies, the heterogeneity of methodologies impedes the identification of the most promising tools for future use in inner ear therapies.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Transduction Efficiency and Immunogenicity of Viral Vectors for Cochlear Gene Therapy: A Systematic Review of Preclinical Animal Studies

Verdoodt

Peeleman

Camp

et al. 2021

Front. Cell. Neurosci.

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“…The current gold standard for the diagnosis and evaluation of sensorineural hearing loss is a functional hearing assessment. There are multiple approaches to assess functional hearing in preclinical studies; therefore, this systematic review will include studies that utilize audiologic tests that are routinely used in experimental studies of hearing loss [16]. Therefore, the primary outcomes will include:Cochlear microphonic receptor potentialsAuditory brainstem response (ABR)ElectrocochleographyDistortion product otoacoustic emissions testing (DPOAE)Summating potentialsTympanometryCompound action potentialsBrainstem auditory evoked potentials (BAEPs)…”

Section: Methodsmentioning

confidence: 99%

Mesenchymal stem cells for sensorineural hearing loss: protocol for a systematic review of preclinical studies

et al. 2019

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Background Sensorineural hearing loss (SNHL) is the most common form of hearing impairment and is characterized by a loss of receptor hair cells and/or spiral ganglion neurons. Regenerative stem cell therapy could potentially restore normal hearing and slow the progression of hearing loss in patients. Preclinical animal studies have demonstrated that mesenchymal stem cells (MSCs) could be a promising new therapy for this condition. These findings have prompted investigators to begin human clinical trials to assess the safety and efficacy of MSCs for the treatment of SNHL. The objective of the proposed systematic review is to examine the efficacy of MSCs as a therapy for SNHL in animal models. Methods We will include preclinical animal studies of SNHL in which MSCs are administered, and outcomes are compared against MSC-naïve controls. The primary outcome will include audiologic tests that are routinely used in experimental studies of hearing loss, such as auditory brainstem response (ABR) and distortion product otoacoustic emissions testing (DPOAE). Secondary outcomes will include histology, microscopy, gene protein expression, and behavioral responses of animals. Electronic searches of MEDLINE via PubMed, Scopus, ScienceDirect, and Cumulative Index to Nursing and Allied Health Literature (CINAHL) will be performed. Search results will be screened independently and in duplicate. Data from eligible studies will be extracted, pooled, and analyzed using random effects models. Risk of bias and publication bias will be assessed using the Systematic Review Center for Laboratory Animal Experimentation (SYRCLE) risk of bias tool and Funnel Plots/Egger’s regression tests, respectively. Discussion This systematic review will provide a summary of the efficacy of MSC therapy in animal models of SNHL, utilizing functional hearing assessment as a primary outcome. Findings from this review are important because they can elucidate research gaps that should be addressed in future preclinical studies and in turn can be translated into clinical studies. Systematic review registration CAMARADES ( http://www.dcn.ed.ac.uk/camarades/ ) Electronic supplementary material The online version of this article (10.1186/s13643-019-1015-7) contains supplementary material, which is available to authorized users.

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“…More than that, an advanced statistical approach is used to check whether the method can be used as an indicator for some fine differences that cannot be perceived by a regular histological investigation or it could be used as a tool for type differentiation. The results provided by such a study can offer valuable information both for human and veterinary pathology, as chinchillas are lately used as experimental animals (Shaffer et al 2013;Mikkelsen et al 2017;Reis et al 2017) for a series of pathological or nonpathological conditions.…”

Section: Introductionmentioning

confidence: 99%

Morphological and morphometrical aspects of intima and media of the common, external and internal iliac arteries in Chinchilla lanigera (Rodentia, Chinchillidae) bred in captivity

Martonos

Damian

Alexandru

et al. 2020

The European Zoological Journal

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The paper approaches deal with fine differential morphological and morphometrical aspects of the structure of three main blood vessels in Chinchilla lanigera: the common iliac artery, the internal iliac artery and the external iliac artery. A series of morphological assessments combined with morphometrical data of the intimal layer, dimensions of the media of the vessels, number of elastic laminae and characteristics observed, alow an interesting conclusion when data is regarded in the light of the classical division of the vessels-of muscular, elastic or transitional type. A complex statistical approach (PostHoc tests) is added to highlight some fine differences in terms of metrical data among these three arteries. The complex statistical investigation brings some interesting facts in terms of series resemblances, leading to a reasonable conclusion that the transition from elastic to muscular type of arteries should not be regarded inflexibly, arteries sharing different features, depending on anatomical sector and species.

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Modelos animais para avaliação auditiva: revisão de literatura

Cited by 16 publications

References 28 publications

Transduction Efficiency and Immunogenicity of Viral Vectors for Cochlear Gene Therapy: A Systematic Review of Preclinical Animal Studies

Transduction Efficiency and Immunogenicity of Viral Vectors for Cochlear Gene Therapy: A Systematic Review of Preclinical Animal Studies

Mesenchymal stem cells for sensorineural hearing loss: protocol for a systematic review of preclinical studies

Morphological and morphometrical aspects of intima and media of the common, external and internal iliac arteries in Chinchilla lanigera (Rodentia, Chinchillidae) bred in captivity

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