Models and Methods for In Vitro Toxicity

Jain, Abhishek Kumar; Singh, Divya; Dubey, Kavita; Maurya, Ranjana; Mittal, Sandeep; Pandey, Alok Kumar

doi:10.1016/b978-0-12-804667-8.00003-1

Cited by 59 publications

(35 citation statements)

References 60 publications

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“…An in vitro method based on cultured cell viability assay was selected for safety screening of the blue passion flower extracts. Such techniques, corresponding to the reduction-replacement-refinement ethical principles of laboratory animal tests, offer results that are consistent with ingestion/in vivo studies [85] and also have the advantages of rapidity and lower costs when compared to the in vivo techniques [86].…”

Section: Cytocompatibility Of the Extracts From The Treated Leavesmentioning

confidence: 93%

Effects of Foliar Treatment with a Trichoderma Plant Biostimulant Consortium on Passiflora caerulea L. Yield and Quality

et al. 2020

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The influence of spore concentration on the ability of a Trichoderma consortium to colonize the Passiflora caerulea phyllosphere was evaluated by determining the effects of foliar treatments with two spore concentrations, in two repeated treatments, on the morphological, physiological, and ultrastructural characteristics, and on the yield and quality of P. caerulea. The studied crop quality features were related to its nutraceutical use: the accumulation of polyphenols and flavonoids, antioxidant activity, and effects on mouse fibroblast L929 cells. The Trichoderma consortium consisted of two strains, T. asperellum T36b and T. harzianum Td50b, and the concentrations used were 106 colony forming units (cfu)/mL and 108 cfu/mL. As a reference treatment, a commercial product that was based on herbs and algal extracts was used. As compared to the negative control, the treatment with the Trichoderma consortium at 108 cfu/mL concentration determines the accumulation of higher level of polyphenols and flavonoids and increased antioxidant activity. This enhancement of P. caerulea quality characteristics after treatment with the higher concentration of Trichoderma consortium was associated with larger leaves, increased number and size of chloroplasts, improved plant physiology characteristics, and an increased yield. The treatment with high concentration of Trichoderma consortium spores promotes phyllosphere colonization and benefits both crop yield and quality.

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Section: Cytocompatibility Of the Extracts From The Treated Leavesmentioning

confidence: 93%

Effects of Foliar Treatment with a Trichoderma Plant Biostimulant Consortium on Passiflora caerulea L. Yield and Quality

et al. 2020

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“…Molecules 2020, 25, x 10 of 14 TA1535_10RLI/TA1535_NA tests on Salmonella typhimurium bacterial strain [67] and mouse [68] carcinogenicity, and Daphnia fish [69] toxicity were carried out.…”

Section: Discussionmentioning

confidence: 99%

“…Toxicology studies were carried out using online implementation [65] of the PreADMET program [66]. From among available statistical models, Ames TA100_10RLI/TA100_NA/TA1535_10RLI/ TA1535_NA tests on Salmonella typhimurium bacterial strain [67] and mouse [68] carcinogenicity, and Daphnia fish [69] toxicity were carried out.…”

Section: Admetmentioning

confidence: 99%

Docking and QSAR of Aminothioureas at the SARS-CoV-2 S-Protein–Human ACE2 Receptor Interface

Płonka

Paneth

2020

Molecules

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Docking of over 160 aminothiourea derivatives at the SARS-CoV-2 S-protein–human ACE2 receptor interface, whose structure became available recently, has been evaluated for its complex stabilizing potency and subsequently subjected to quantitative structure–activity relationship (QSAR) analysis. The structural variety of the studied compounds, that include 3 different forms of the N–N–C(S)–N skeleton and combinations of 13 different substituents alongside the extensive length of the interface, resulted in the failure of the QSAR analysis, since different molecules were binding to different parts of the interface. Subsequently, absorption, distribution, metabolism, and excretion (ADME) analysis on all studied compounds, followed by a toxicity analysis using statistical models for selected compounds, was carried out to evaluate their potential use as lead compounds for drug design. Combined, these studies highlighted two molecules among the studied compounds, i.e., 5-(pyrrol-2-yl)-2-(2-methoxyphenylamino)-1,3,4-thiadiazole and 1-(cyclopentanoyl)-4-(3-iodophenyl)-thiosemicarbazide, as the best candidates for the development of future drugs.

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“…However, reliable in vitro testing methods to measure drug toxicity in the testis are lacking. In recent years, in vitro toxicity models have reduced the use of experimental animals and saved considerable time and resources (Jain et al, 2018). Evaluating spermatogenesis is a common and important endpoint for testicular toxicity.…”

Section: Introductionmentioning

confidence: 99%

Gene expression profiling of cultured mouse testis fragments treated with ethinylestradiol

2019

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The assessment of xenobiotic-induced testicular toxicity is important in drug development. Nonetheless, in vitro models to test drugs and chemicals that may cause testicular toxicity are lacking, requiring the continued use of animal models for those studies. We previously evaluated an in vitro mouse testis organ culture system using ethinylestradiol (EE), a well-studied testicular toxicant, and demonstrated a dose-dependent relationship between adverse effects to germ cell differentiation and increasing EE concentrations. However, we terminated that study after 20 days of culture due to oxygen deficiency during germ cell differentiation. Therefore, in the current study, we aimed to identify gene(s) with potential for supporting the histopathological evaluations of testicular toxicity using in vitro testis organ culture system. We cultured testis fragments obtained from mice at postnatal day (PND) 5 in α-Minimal Essential Medium containing 40 mg/mL AlbuMAX ™ I and treated them with 0.01 or 1 nM EE on day 1 of culture. On day 20, we collected testis fragments for RNA sequencing analysis and quantitative polymerase chain reaction (qPCR). We found that phospholipase C, zeta 1 and testis-specific serine kinase 4 genes, that are involved in spermatogenesis and predominantly expressed in the testis, were significantly reduced in testis fragments treated with the highest concentration of EE. Also, cytochrome P450, family 26, subfamily b, polypeptide 1 (Cyp26b1) and interleukin 16 (Il16) were up-regulated in the highest EE-treated groups. Further studies are needed to confirm the variations of these gene expression using other testicular toxicants.

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Models and Methods for In Vitro Toxicity

Cited by 59 publications

References 60 publications

Effects of Foliar Treatment with a Trichoderma Plant Biostimulant Consortium on Passiflora caerulea L. Yield and Quality

Effects of Foliar Treatment with a Trichoderma Plant Biostimulant Consortium on Passiflora caerulea L. Yield and Quality

Docking and QSAR of Aminothioureas at the SARS-CoV-2 S-Protein–Human ACE2 Receptor Interface

Gene expression profiling of cultured mouse testis fragments treated with ethinylestradiol

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