Models and Tools for Investigating Eosinophilic Esophagitis at the Bench

Uchida, Amiko M.; Ro, Gabrielle; Garber, John J.; Peterson, Kathryn A.; Round, June L.

doi:10.3389/fimmu.2022.943518

Cited by 4 publications

(3 citation statements)

References 73 publications

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“…En nuestro caso, ante la ausencia de manifestaciones hemorrágicas previas al procedimiento y con el antecedente de no requerir tratamiento o profilaxis, se decidió la toma de biopsias por el hallazgo casual de lesiones compatibles con una EoE, procedimiento realizado sin ningún tipo de complicaciones. En una revisión realizada por Uchida y colaboradores [12], se demuestra el gran interés que ha surgido en descubrir biomarcadores no invasivos en suero para el estudio de la EoE; hasta ahora se han descrito aumentos de la eotaxina-3, canales de cloruro (CLC, del inglés, Chloride Channel), proteína catiónica del eosinófilo (ECP, del inglés, Eosinophil Cationic Protein), neurotoxina derivada de eosinófilos (EDN, del inglés Eosinophil-Derived Neurotoxin), proteína básica de mielina (MBP, del inglés, Myelin Basic Protein), interleuquina 15 (IL-15) y factor de crecimiento transformante beta-1 (TGF-β1).…”

Section: Discussionunclassified

Seguridad de la endoscopia y biopsia esofágica en un paciente con esofagitis eosinofílica y enfermedad de Von Willebrand

Herrera-Hoyos¹,

Ortiz-Gualdrón²

2023

Med. Lab.

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La esofagitis eosinofílica (EoE) es una enfermedad causada por una respuesta inmune frente a antígenos alimentarios en contacto con la mucosa esofágica; por su parte, la enfermedad de Von Willebrand (EVW) es el trastorno hemorrágico hereditario más común en los seres humanos. La característica central de todos los tipos de EVW, es la presencia de cantidades reducidas o de formas anormales del factor de Von Willebrand (FVW) en el torrente sanguíneo. Debido a que no se han reportado casos previos de EVW tipo 2A asociada a EoE, se describe este caso clínico con el objetivo principal de dar a conocer el hallazgo casual de estas dos patologías, la seguridad de la evaluación por endoscopia de vías digestivas altas y el pronóstico de posibles complicaciones.

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Section: Discussionunclassified

Seguridad de la endoscopia y biopsia esofágica en un paciente con esofagitis eosinofílica y enfermedad de Von Willebrand

Herrera-Hoyos¹,

Ortiz-Gualdrón²

2023

Med. Lab.

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“…One of these directions may be the search for miRNA-4668 in salivary and esophageal secretions. The increased expression of miRNA-4668 was found in the saliva of patients with EoE [ 73 ]. Another marker may be the spectrometric test for the presence of 3-Bromotyrosine in urine; 3-Bromotyrosine is a marker of eosinophil activation and its increased concentration was found in patients with EoE [ 74 ].…”

Section: Diagnosticsmentioning

confidence: 99%

Eosinophilic Esophagitis—What Do We Know So Far?

Wąsik

Małecka-Wojciesko

2023

JCM

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Eosinophilic esophagitis is a Th-2 antigen-mediated disease in which there is an influx of eosinophils to all layers of the esophagus, triggering an inflammatory response. Chronic inflammatory process causes esophageal remodeling, leading to difficulties in swallowing. Food impaction, heartburn, and chest pain are other characteristic (but not pathognomonic) symptoms in adults. Although the disease has only been described since in the early 1970s, its incidence and prevalence are rapidly growing, especially in Western countries. According to the diagnostic guidelines, there should be at least 15 eosinophils visible per high-power field in biopsies obtained from different sites in the esophagus upon endoscopy with relevant esophageal symptoms. Other diseases that can cause esophageal eosinophilia should be ruled out. Eosinophilic esophagitis treatment may be challenging; however, new methods of management have recently emerged. The currently used proton pump inhibitors, topical corticosteroids, and elimination diet are combined with biological treatment. New methods for disease diagnostics and clinical course assessment are also available. This review presents current knowledge about the disease, supported by the latest research data.

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“…Importantly, non-invasive biomarkers of EoE have not yet been identified ( 14 ), and no immune signature specific for EoE has been described in blood ( 15 ). Interestingly, a preliminary study performed targeted metabolomics by analysing 48 metabolites in plasma from a small cohort ( 16 ).…”

Section: Introductionmentioning

confidence: 99%

Assessment of local and systemic signature of eosinophilic esophagitis (EoE) in children through multi-omics approaches

et al. 2023

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BackgroundEosinophilic oesophagitis (EoE) is a chronic food allergic disorder limited to oesophageal mucosa whose pathogenesis is still only partially understood. Moreover, its diagnosis and follow-up need repeated endoscopies due to absence of non-invasive validated biomarkers. In the present study, we aimed to deeply describe local immunological and molecular components of EoE in well-phenotyped children, and to identify potential circulating EoE-biomarkers.MethodsBlood and oesophageal biopsies were collected simultaneously from French children with EoE (n=17) and from control subjects (n=15). Untargeted transcriptomics analysis was performed on mRNA extracted from biopsies using microarrays. In parallel, we performed a comprehensive analysis of immune components on both cellular and soluble extracts obtained from both biopsies and blood, using flow cytometry. Finally, we performed non-targeted plasma metabolomics using liquid chromatography coupled to high-resolution mass spectrometry (LC-HRMS). Uni/multivariate supervised and non-supervised statistical analyses were then conducted to identify significant and discriminant components associated with EoE within local and/or systemic transcriptomics, immunologic and metabolomics datasets. As a proof of concept, we conducted multi-omics data integration to identify a plasmatic signature of EoE.ResultsFrench children with EoE shared the same transcriptomic signature as US patients. Network visualization of differentially expressed (DE) genes highlighted the major dysregulation of innate and adaptive immune processes, but also of pathways involved in epithelial cells and barrier functions, and in perception of chemical stimuli. Immune analysis of biopsies highlighted EoE is associated with dysregulation of both type (T) 1, T2 and T3 innate and adaptive immunity, in a highly inflammatory milieu. Although an immune signature of EoE was found in blood, untargeted metabolomics more efficiently discriminated children with EoE from control subjects, with dysregulation of vitamin B6 and various amino acids metabolisms. Multi-blocks integration suggested that an EoE plasma signature may be identified by combining metabolomics and cytokines datasets.ConclusionsOur study strengthens the evidence that EoE results from alterations of the oesophageal epithelium associated with altered immune responses far beyond a simplistic T2 dysregulation. As a proof of concept, combining metabolomics and cytokines data may provide a set of potential plasma biomarkers for EoE diagnosis, which needs to be confirmed on a larger and independent cohort.

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Models and Tools for Investigating Eosinophilic Esophagitis at the Bench

Cited by 4 publications

References 73 publications

Seguridad de la endoscopia y biopsia esofágica en un paciente con esofagitis eosinofílica y enfermedad de Von Willebrand

Seguridad de la endoscopia y biopsia esofágica en un paciente con esofagitis eosinofílica y enfermedad de Von Willebrand

Eosinophilic Esophagitis—What Do We Know So Far?

Assessment of local and systemic signature of eosinophilic esophagitis (EoE) in children through multi-omics approaches

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