Models for the Study of Angiogenesis

Shiba, Yuji; Takahashi, Masafumi; Ikeda, Uichi

doi:10.2174/138161208783497741

Cited by 11 publications

(5 citation statements)

References 54 publications

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“…The Matrigel assay is commonly considered to be an experimental method that measures the ability of cells to promote angiogenesis in vitro (Shiba et al, ). To prove that hypoxia treatment did indeed stimulate the production of angiogenic growth factors by AD‐MSCs, we explored whether hypo‐CM upregulated the formation of capillary‐like structures in HUVECs on Matrigel in vitro.…”

Section: Resultsmentioning

confidence: 99%

Hypoxia preconditioned human adipose derived mesenchymal stem cells enhance angiogenic potential via secretion of increased VEGF and bFGF

Liu

Gao

Yuan

et al. 2013

Cell Biology International

144

103

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Mesenchymal stem cells (MSCs) are adult multipotent cells found in bone marrow, adipose tissue, and other adult tissues. MSCs improve regeneration of injured tissues in vivo, but the mechanisms remain unclear. Typically, MSCs are cultured under ambient or normoxic conditions (21% O2 ). However, the physiological niches of MSCs have much lower oxygen tension. When used as a therapeutic tool to repair tissue injuries, MSCs cultured in standard conditions must adapt from 21% O2 in culture to <1% O2 in ischemic tissue. We have examined the effects of hypoxia preconditioning (1% O2 ) in human adipose derived mesenchymal stem cells (AD-MSCs) to discover the conditions that best enhance their tissue regenerative potential. We demonstrate that AD-MSCs respond positively to hypoxia compared with normoxia preconditioning, show decreased apoptosis even in severe microenvironmental conditions (such as a low-serum medium), and an increased expression of the angiogenic factors, vascular endothelial growth factor and basic fibroblast growth factor. Human umbilical vein endothelial cells have higher vitality and lower apoptosis when cultured in medium taken from hypoxia-preconditioned AD-MSCs, as well as significantly increased capillary-like structures with this medium on Matrigel. The data suggest that hypoxia preconditioned AD-MSCs can improve tissue regeneration.

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Section: Resultsmentioning

confidence: 99%

Hypoxia preconditioned human adipose derived mesenchymal stem cells enhance angiogenic potential via secretion of increased VEGF and bFGF

Liu

Gao

Yuan

et al. 2013

Cell Biology International

144

103

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show abstract

“…The Matrigel assay is commonly considered to be an experimental method that measures the ability of cells to promote angiogenesis in vitro [15] and is also used to support the differentiation of neural precursor cells into neuronal cells [16] . NGF is a common neurotrophic factor, so we needed to exclude the possibility that MSCs normally have the ability to differentiate into neuronal cells.…”

Section: Discussionmentioning

confidence: 99%

“…Cord formation was induced using Matrigel (Sigma) [15] . For the MSC cord formation assay, 200 µL of Matrigel was paved on a well of a 24-well plate and incubated for 1 h at 37 °C.…”

Section: Matrigel Assaymentioning

confidence: 99%

Nerve growth factor induces cord formation of mesenchymal stem cell by promoting proliferation and activating the PI3K/Akt signaling pathway

Wang

Xie

et al. 2011

Acta Pharmacol Sin

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Aim: To investigate whether nerve growth factor (NGF) induced angiogenesis of bone marrow mesenchymal stem cells (MSCs) and the underlying mechanisms. Methods: Bone marrow MSCs were isolated from femors or tibias of Sprague-Dawley rat, and cultured. The cells were purified after 3 to 5 passages, seeded on Matrigel-coated 24-well plates and treated with NGF. Tube formation was observed 24 h later. Tropomyosinrelated kinase A (TrkA) and p75NTR gene expression was examined using PCR analysis and flow cytometry. Growth curves were determined via cell counting. Expression of VEGF and pAkt/Akt were analyzed with Western blot.

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“…To screen the effects of environmental chemicals in tumor angiogenesis, there are many well developed in vitro and in vivo angiogenesis model systems that can be used or adapted (138)(139)(140)(141)(142)(143)(144)(145)(146)(147)(148)(149)(150)(151). Each model has distinct advantages and disadvantages.…”

Section: In Vitro and In Vivo Angiogenesis Assays Including Hts Assaymentioning

confidence: 99%

Assessing the carcinogenic potential of low-dose exposures to chemical mixtures in the environment: focus on the cancer hallmark of tumor angiogenesis

Hu¹,

Brooks

Dormoy

et al. 2015

CARCIN

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One of the important 'hallmarks' of cancer is angiogenesis, which is the process of formation of new blood vessels that are necessary for tumor expansion, invasion and metastasis. Under normal physiological conditions, angiogenesis is well balanced and controlled by endogenous proangiogenic factors and antiangiogenic factors. However, factors produced by cancer cells, cancer stem cells and other cell types in the tumor stroma can disrupt the balance so that the tumor microenvironment favors tumor angiogenesis. These factors include vascular endothelial growth factor, endothelial tissue factor and other membrane bound receptors that mediate multiple intracellular signaling pathways that contribute to tumor angiogenesis. Though environmental exposures to certain chemicals have been found to initiate and promote tumor development, the role of these exposures (particularly to low doses of multiple substances), is largely unknown in relation to tumor angiogenesis. This review summarizes the evidence of the role of environmental chemical bioactivity and exposure in tumor angiogenesis and carcinogenesis. We identify a number of ubiquitous (prototypical) chemicals with disruptive potential that may warrant further investigation given their selectivity for high-throughput screening assay targets associated with proangiogenic pathways. We also consider the cross-hallmark relationships of a number of important angiogenic pathway targets with other cancer hallmarks and we make recommendations for future research. Understanding of the role of low-dose exposure of chemicals with disruptive potential could help us refine our approach to cancer risk assessment, and may ultimately aid in preventing cancer by reducing or eliminating exposures to synergistic mixtures of chemicals with carcinogenic potential.

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Models for the Study of Angiogenesis

Cited by 11 publications

References 54 publications

Hypoxia preconditioned human adipose derived mesenchymal stem cells enhance angiogenic potential via secretion of increased VEGF and bFGF

Hypoxia preconditioned human adipose derived mesenchymal stem cells enhance angiogenic potential via secretion of increased VEGF and bFGF

Nerve growth factor induces cord formation of mesenchymal stem cell by promoting proliferation and activating the PI3K/Akt signaling pathway

Assessing the carcinogenic potential of low-dose exposures to chemical mixtures in the environment: focus on the cancer hallmark of tumor angiogenesis

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