Models of Inflammation: Carrageenan Air Pouch

Fehrenbacher, Jill C.; McCarson, Kenneth E.

doi:10.1002/cpz1.183

Cited by 18 publications

(9 citation statements)

References 51 publications

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“…The mouse model of chronic inflammation used in the current study was adapted from well known models that utilize carrageenan to induce an inflammatory response for testing anti-inflammatory drugs [31]. A chronic immune response was induced through repeated administration of carrageenan at the site of phantom implantation over two weeks.…”

Section: Discussionmentioning

confidence: 99%

In vivo Biomedical Imaging of Immune Tolerant, Radiopaque Nanoparticle-Embedded Polymeric Device Degradation

Pawelec,

Hix,

Troia

et al. 2023

Preprint

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Biomedical implants remain an important clinical tool for restoring patient mobility and quality of life after trauma. While polymers are often used for devices, their degradation profile remains difficult to determine post-implantation. CT monitoring could be a powerful tool for in situ monitoring of devices, but polymers require the introduction of radiopaque contrast agents, like nanoparticles, to be distinguishable from native tissue. As device function is mediated by the immune system, use of radiopaque nanoparticles for serial monitoring therefore requires a minimal impact on inflammatory response. Radiopaque polymer composites were produced by incorporating 0-20wt% TaOxnanoparticles into synthetic polymers: polycaprolactone (PCL) and poly(lactide-co-glycolide) (PLGA). In vitro inflammatory response to TaOxwas determined by monitoring mouse bone marrow derived macrophages on composite films. Nanoparticle addition stimulated only a slight inflammatory reaction, namely increased TNFα secretion, mediated by changes to the polymer matrix properties. When devices (PLGA 50:50 + 20wt% TaOx) were implanted subcutaneously in a mouse model of chronic inflammation, no changes to device degradation were noted although macrophage number was increased over 12 weeks. Serial CT monitoring of devices post-implantation provided a detailed timeline of device structural collapse, with no burst release of the nanoparticles from the implant. Changes to the device were not significantly altered with monitoring, nor was the immune system ablated when checked via blood cell count and histology. Thus, polymer devices incorporating radiopaque TaOxNPs can be used for in situ CT monitoring, and can be readily combined with multiple medical imaging techniques, for a truly dynamic view biomaterials interaction with tissues throughout regeneration, paving the way for a more structured approach to biomedical device design.

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Section: Discussionmentioning

confidence: 99%

In vivo Biomedical Imaging of Immune Tolerant, Radiopaque Nanoparticle-Embedded Polymeric Device Degradation

Pawelec,

Hix,

Troia

et al. 2023

Preprint

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“…In this study, a carrageenan-induced air pouch was established according to the previous study [19], rats were randomly divided into four groups of eight rats each. The experiment was performed over six days.…”

Section: Experimental Designmentioning

confidence: 99%

Vildagliptin attenuates carrageenan-induced air pouch inflammation in rats via modulation of COX-2/ PGF-2α and NF-κB signaling cascade

Fawzy,

el kholy,

Hosny

et al. 2024

ERU Research Journal

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The carrageenan-induced-air pouch is a particularly good model for evaluating the relationship between inflammation and its related vital mediators. Vildagliptin is an active dipeptidyl peptidase-4 (DPP-4) inhibitor, which is known to possess an anti-hyperglycemic efficacy. The present study was established to investigate the effect of vildagliptin on the carrageenan-induced air pouch model of inflammation. Four groups of rats, each containing eight rats, were used. The experiment was performed over six days. Starting from the first day of the experiment, an air pouch was established on alternate days in the four groups by injecting 20 ml of sterile air subcutaneously into the rat intracapsular area. On the last day of the experiment, rats in the third and fourth groups received vildagliptin 5, and 10 mg/kg, respectively, orally half an hour before carrageenan was given. The second group received indomethacin (10 mg/kg) half an hour before carrageenan was given. Three hours later, the air pouch was opened and the entire exudate of each animal was harvested to assess myeloperoxidase (MPO) & superoxide dismutase (SOD) activities, tumor necrosis factor-alpha (TNF-α), nuclear factor kappa-B (NF-ᴋB), cyclooxygenase 2(COX-2), prostaglandin F2-alpha (PGF-2α), interleukin 10 (IL-10) levels.Vildagliptin 5 mg/kg, 10 mg/Kg, and indomethacin significantly suppressed the NF-ᴋB signaling pathway, reduced TNF-α, MPO, COX-2, PGF2-α levels, while enhancing that of IL-10, and SOD ERURJ 2024, x, x, Accepted 2 activity. The findings of this study revealed that vildagliptin has an inhibitory effect on inflammation in carrageenan-mediated air pouch in rats by inhibiting COX-2/PGF2-α activity and suppressing the NF-kB signaling pathway.

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“…On the other hand, carrageenans also display their own biological activities, including antiviral, antibacterial, and antihyperlipidemic activities [ 163 ]. They are also well recognized and widely applied in experimental procedures involving inflammation, as they are used to induce inflammatory processes in laboratory animals [ 164 ]. Biological activities of carrageenans are also a source of some toxicity-related concerns.…”

Section: Gum-based Micro- and Nanoparticles In Drug Deliverymentioning

confidence: 99%

Natural Gums in Drug-Loaded Micro- and Nanogels

et al. 2023

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Gums are polysaccharide compounds obtained from natural sources, such as plants, algae and bacteria. Because of their excellent biocompatibility and biodegradability, as well as their ability to swell and their sensitivity to degradation by the colon microbiome, they are regarded as interesting potential drug carriers. In order to obtain properties differing from the original compounds, blends with other polymers and chemical modifications are usually applied. Gums and gum-derived compounds can be applied in the form of macroscopic hydrogels or can be formulated into particulate systems that can deliver the drugs via different administration routes. In this review, we present and summarize the most recent studies regarding micro- and nanoparticles obtained with the use of gums extensively investigated in pharmaceutical technology, their derivatives and blends with other polymers. This review focuses on the most important aspects of micro- and nanoparticulate systems formulation and their application as drug carriers, as well as the challenges related to these formulations.

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Models of Inflammation: Carrageenan Air Pouch

Cited by 18 publications

References 51 publications

In vivo Biomedical Imaging of Immune Tolerant, Radiopaque Nanoparticle-Embedded Polymeric Device Degradation

In vivo Biomedical Imaging of Immune Tolerant, Radiopaque Nanoparticle-Embedded Polymeric Device Degradation

Vildagliptin attenuates carrageenan-induced air pouch inflammation in rats via modulation of COX-2/ PGF-2α and NF-κB signaling cascade

Natural Gums in Drug-Loaded Micro- and Nanogels

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