Moderate and severe neutropenia in patients with systemic lupus erythematosus

Martínez-Baños, Déborah; Crispín, José C.; Lazo‐Langner, Alejandro; Sánchez‐Guerrero, Jorge

doi:10.1093/rheumatology/kel016

Cited by 51 publications

(40 citation statements)

References 14 publications

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“…Both internal and external factors were reported to contribute to the development of neutropenia. Intrinsic factors such as thrombocytopenia, lupus activity in central nervous system, and circulating autoantibodies and extrinsic factors such as use of immunosuppressive drugs and concomitant infections were reported as risk factors for neutropenia in SLE patients [6,10,[20][21][22]. Antineutrophil antibodies were shown to be directly involved in the peripheral destruction of neutrophils in SLE patients [7,23,24].…”

Section: Discussionmentioning

confidence: 98%

“…Lymphopenia is a most commonly found leukocyte abnormality, but neutropenia is also frequently observed occurring in about 50% to 60% of patients with active SLE [5]. Most of the neutropenia episodes occurring in patients with SLE are mild, but approximately 5% of patients may experience moderate (500/mm 3 ≤absolute neutrophil count [ANC] ＜1,000/mm 3 ) to severe neutropenia (ANC＜500/mm 3 ) [6]. The pathogenesis of neutropenia in SLE is not entirely understood, but both humoral and cellular immune responses are known to be involved.…”

Section: Introductionmentioning

confidence: 99%

“…It is crucial to understand the risk factors associated with neutropenia in SLE patients, but there is a paucity of clinical data regarding neutropenia in patients with SLE. Although many studies deal with hematologic abnormalities in SLE [14], only one previous study showed clinical data focusing on neutropenia reporting risk factors associated with neutropenia in 33 SLE patients with moderate and severe neutropenia [6]. They reported that concomitant medications including immunosuppressants, history of thrombocytopenia and central nervous systemic manifestation as risk factors of developing neutropenia in SLE.…”

Section: Introductionmentioning

confidence: 99%

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Prevalence and Risk Factors Associated with Neutropenia in Hospitalized Patients with Systemic Lupus Erythematosus

2017

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Section: Discussionmentioning

confidence: 98%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Prevalence and Risk Factors Associated with Neutropenia in Hospitalized Patients with Systemic Lupus Erythematosus

2017

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“…Peneliti telah melaporkan bahwa sebagian besar penderita SLE aktif mempunyai jumlah leukosit menurun atau leukopenia. 13,14 Bahkan, leukopenia dan granulositopenia tidak jarang ditemukan pada pasien SLE di berbagai negara di belahan dunia. 13,14 Perbedaan hasil pemeriksaan hematologi ini kemungkinan besar disebabkan oleh remisi penyakit SLE setelah pasien minum obat-obat imunosupresif (data tidak ditunjukkan).…”

Section: Pembahasanunclassified

Ekspresi CD3 dan CD26 pada Limfosit T sebagai Biomarker Potensial Penyakit Systemic Lupus Erythematosus

Suselo¹,

Balgis²,

Indarto³

2016

mkb

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AbstrakSystemic lupus erythematosus (SLE) merupakan penyakit autoimun yang sering dijumpai pada wanita. Penyakit ini ditandai oleh hiperautoreaktivitas limfosit T dan B. Di dalam sistem imun, CD3 dibantu CD26 sebagai molekul kostimulator berkaitan erat dengan aktivasi dan migrasi limfosit T. Pada penyakit SLE, ekspresi CD3 dan CD26 serta aktivitas enzim CD26 belum diketahui. Tujuan penelitian ini adalah mengetahui ekspresi CD3 dan CD26 dalam darah serta kultur limfosit T pasien SLE. Rancangan penelitian ini bersifat eksperimen laboratorium dengan pendekatan studi retrospektif. Penelitian dilakukan di Laboratorium Biomedik, Fakultas Kedokteran Universitas Sebelas Maret Surakarta selama lima bulan (Mei-September 2012). Diagnosis SLE ditentukan menurut kriteria dari American College of Rheumatology (ACR). Darah vena diambil dari tiga pasien SLE dan dua orang sehat. Satu µg/mL phytohaemmaglutinin (PHA) digunakan untuk stimulasi kultur limfosit T. Ekspresi CD3 dan CD26 ditentukan dengan flows sytometry. Substrat H-Gly-Pro pNA digunakan untuk menguji aktivitas enzim CD26. Data yang terkumpul dianalisis dengan uji t. Ekspresi CD3 dan CD26 menurun dalam darah dan kultur limfosit T pada pasien SLE dibanding dengan kontrol, sedangkan aktivitas enzim CD26 pada kultur limfosit T pasien SLE lebih tinggi daripada kontrol (0.042 vs 0.030 U/mL), tetapi perbedaan tersebut tidak bermakna secara statistik (p>0.05). Simpulan, terdapat penurunan ekspresi CD3 dan CD26 baik dalam sirkulasi darah maupun di kultur limfosit T subtipe CD4+. CD3 dan CD26 berpotensi sebagai biomarker penting untuk SLE. Namun, riset lanjutan masih perlu dilakukan untuk menjelaskan peran keduanya dalam patogenesis penyakit SLE. [MKB. 2016;48(3):140-7] Kata kunci: CD3, CD26, systemic lupus erythematosus (SLE) CD3 and CD26 Expression on T Lymphocytes as a Potential Biomarker of Systemic Lupus Erithematosus AbstractSystemic lupus erythematosus (SLE) is an autoimmune disease that is frequently found in women and characterized by hyperautoreactivity of T and B cells. In the immune system, expressions of CD 3 and CD26 (as co-stimulatory molecule) are related to T cells activation and migration. Co-expression of CD3 and CD26 in SLE patients has not been determined. The aim of this study was to investigate the co-expression of CD3 and CD26 in blood and T cell culture of SLE patients. This was an analytical descriptive study with a retrospective approach. This study was performed at the Biomedical laboratory, Faculty of Medicine, Sebelas Maret University, for five months (May-September 2012). SLE diagnosis was determined by using the criteria from the American College of Rheumatology (ACR). Vein blood was collected from three female patients with SLE and two healthy female controls. T cells isolated from the blood were cultured and stimulated with 1 µg/mL phytohaemmaglutinin (PHA). Flow cytometry was used to determine the coexpression of CD3 and CD26. CD26 enzyme activities in T cell culture were spectrophotometrically measured using H-Gly-Pro pNA substrate. Colle...

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“…Despite chronic neutropenia and hyper-susceptibility to bacterial infections [15][16][17], a significant proportion of SLE patients display elevated levels of immature neutrophils and a pronounced granulopoiesis gene expression signature in cells from both peripheral blood and bone marrow exudates [2,18,19]. In addition, neutrophils are known to infiltrate target organs such as the skin, kidney or vasculature in SLE patients [20][21][22], although the pathogenic nature of such infiltration remains unknown.…”

Section: Pro-inflammatory Neutrophils In Accumulate In Sle Patients Amentioning

confidence: 99%

Pro- and Anti-inflammatory Neutrophils in Lupus

Der¹,

Trigunaite²,

Khan³

et al. 2014

J Clin Cell Immunol

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A hallmark of SLE is the presence of elevated levels of circulating anti-nuclear autoantibodies specific towards chromatin, histones or dsDNA. Understanding the regulation of antibody production is therefore of utmost importance in understanding lupus pathogenesis. Spearheaded by the identification of accumulating immunosuppressive neutrophils in cancer patients, the nature and function of neutrophils have expanded from a uniform pro-inflammatory cell population to a heterogeneous population of cells with pro-inflammatory or immunosuppressive capacities. While much is known about pro-inflammatory neutrophils and the likely pathogenic function of such cells in lupus, a potential role for immunosuppressive neutrophils in protecting genetically predisposed individuals has only recently emerged. For example, SLE-derived neutrophils spontaneously produce type I interferons (IFNα), strongly associated with disease development, release chromatin-containing neutrophil extracellular traps (NETs), potentially functioning as a source of nuclear auto-antigen, and may activate B cells in a T cell independent fashion. In contrast, levels and functions of regulatory neutrophils (Nregs) involved in T celldependent B cell differentiation and germinal center reactions, are dysregulated in female lupus-prone mice during disease development. Here we review data supporting a role for both pro-and anti-inflammatory neutrophils in lupus.

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Moderate and severe neutropenia in patients with systemic lupus erythematosus

Abstract: Most episodes of severe granulocytopenia in SLE patients occur as part of drug toxicity-induced medullar hypoplasia.

Cited by 51 publications

References 14 publications

Prevalence and Risk Factors Associated with Neutropenia in Hospitalized Patients with Systemic Lupus Erythematosus

Prevalence and Risk Factors Associated with Neutropenia in Hospitalized Patients with Systemic Lupus Erythematosus

Ekspresi CD3 dan CD26 pada Limfosit T sebagai Biomarker Potensial Penyakit Systemic Lupus Erythematosus

Pro- and Anti-inflammatory Neutrophils in Lupus

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