It shows searching for a solution to the issue of the stock rebuilding of the Red List species — the Sakhalin sturgeon. It describes the results of the expedition to the Tumnin river aimed at brood fish procurement and optimization of the technology for its reproduction together with the brood fish grown in aquaculture. It lists the morphological characteristics and fish-cultural and bio-data of the spawner caught in the Tumnin river and the milters grown at the Anyuy Sturgeon Fish Hatchery. A method for the obtainment of reproductive products, hatching and juvenile fish growning is described. As a result of sampling for biopsy, it has been found that the coefficient of oocytesʼ nucleus polarization was 7.4 on the average. The hormonal preparation surfagon was used for sex products stimulation at males and a female. The female ovulated in 12 hours after permitting injection. It was managed to get 2,378 g eggs on the average 87.6 per cent of fertilization. The average ejaculate volume was 455 ml. The spermatozoon mobility at mean 14 °C temperature made up 208 sec. A double fertilization method was used at fertilization of an eggs part, moreover the eggs received were of a high piscicultural quality (99%). The average diameter of swollen eggs was maximum 6.8 mm and minimum 6.6 mm after fertilization. The larvae production made up to 30%, i.e. 27,850 of incubated eggs. 1000 ongrown fry were stocked into the Tumnin river, the last juveniles were directed to fish cultural farms of Russia with a view to form there recruitment-and-brood stocks. According to results of experimental works, done in the Anyuy Fish Hatchery, the first provisional technology of work was recommended on fertilized eggs production from the Sakhalin sturgeon spawners after the schema “a wild female + farmed males” by a method of a double fertilization with the increased exposition time.