Modern day breeding approaches for improvement of castor

Patel, Rumit; Menon, Juned; Kumar, Sushil; Nóbrega, Márcia B.M.; Patel, Dipak A.; Sakure, Amar A.; Vaja, Mahesh B.

doi:10.1016/j.heliyon.2024.e27048

Cited by 2 publications

(1 citation statement)

References 102 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Castor bean, as a heat-loving crop, is predominantly cultivated in tropical, subtropical, and temperate regions. India, China, and Brazil are the world’s leaders in the commercial cultivation of castor bean seeds, accounting for more than 90% of the total harvested area [ 2 ]. In 2022, castor bean as an oilseed crop was grown in Russia’s southern zone (Krasnodar and Stavropol territories, as well as the Rostov region); however, its total harvested area was scanty and amounted to only 107 hectares [ 1 ].…”

Section: Introductionmentioning

confidence: 99%

An Efficient and Rapid Protocol for Somatic Shoot Organogenesis from Juvenile Hypocotyl-Derived Callus of Castor Bean cv. Zanzibar Green

Demidenko,

Varlamova,

Soboleva

et al. 2024

BioTech

View full text Add to dashboard Cite

Aseptic seedlings of different ages derived from surface-sterilized mature seeds were applied as an explant source. Various explants such as 7- and 21-day-old hypocotyl fragments, 42-day-old nodal stem segments, and transverse nodal segments of stem, as well as leaf petioles, were cultured on the agar-solidified Murashige and Skoog (MS) basal medium supplemented with 0.1 mg/L IAA, 5 mg/L AgNO3 and different types and concentrations of cytokinin (1 mg/L zeatin, 0.25 mg/L thidiazuron (TDZ), and 5 mg/L 6-benzylaminopurine (6-BAP)). Consequently, it was found that 7- and 21-day-old hypocotyl fragments, as well as nodal stem segments obtained from adult aseptic seedlings, are characterized by a high explant viability and callus formation capacity with a frequency of 79.7–100%. However, the success of in vitro somatic shoot organogenesis was significantly determined not only by the culture medium composition and explant type but also depending on its age, as well as on the size and explant preparation in cases of hypocotyl and age-matched nodal stem fragments, respectively. Multiple somatic shoot organogenesis (5.7 regenerants per explant) with a frequency of 67.5% was achieved during 3 subcultures of juvenile hypocotyl-derived callus tissue on MS culture medium containing 0.25 mg/L TDZ as cytokinin source. Castor bean regenerants were excised from the callus and successfully rooted on ½ MS basal medium without exogenous auxin (81%). In vitro plantlets with well-developed roots were adapted to ex vitro conditions with a frequency of 90%.

show abstract

Section: Introductionmentioning

confidence: 99%