Modern Methods for Characterising, Identifying and Detecting Bacteria Associated With Plants

Stead, D. E.; Elphinstone, J. G.; Weller, Simon A.; Smith, N. C.; Hennessy, J.

doi:10.17660/actahortic.2000.530.4

Cited by 14 publications

(4 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The recent advances of DNA sequencing and fingerprinting technologies have facilitated the detection and identification of target microbes in the isolation process to consequently reduce the uses of selective media and morpho-physiological tests (Stead et al 2000;Bashan et al 2004). DNA sequencing of the 16S ribosomal RNA gene (rDNA) is the most frequently used molecular method for the detection and identification, but it is costly and time consuming for the screening of numerous isolates as compared with other methods.…”

Section: Correspondencementioning

confidence: 99%

See 1 more Smart Citation

A rapid and simple PCR method for identifying isolates of the genus Azospirillum within populations of rhizosphere bacteria

Shime-Hattori

Kobayashi

Ikeda³

et al. 2011

Journal of Applied Microbiology

View full text Add to dashboard Cite

Aims: To develop a rapid and simple genus‐specific polymerase chain reaction (PCR) method for detecting and identifying isolates of the genus Azospirillum which is well‐recognized as plant growth‐promoting rhizobacterium. Methods and Results: Nine pairs of PCR primers were designed based on the Azospirillum 16S rRNA, ipdC, nifA and nifH genes to assess their genus specificity by testing against 12 Azospirillum (from seven species) and 15 non‐Azospirillum reference strains, as compared with the fAZO/rAZO pair reported by Baudoin et al. (J Appl Microbiol, 108, 2010, 25). Among the primer pairs assessed, the Az16S–A pair designed on the 16S rRNA gene sequence showed the highest genus specificity: it successfully yielded a single amplicon of the expected size in all the 12 Azospirillum strains and for a close relative, Rhodocista centenaria. The PCR with the Az16S–A primers generated a detectable amount of the amplicon from ≥103 CFU ml−1 of Azospirillum cell suspensions even in the presence of contaminants and accurately discriminated Azospirillum and non‐Azospirillum species in both 35 Azospirillum‐like and 70 unknown isolates from plant roots and rhizosphere soils. Conclusions: We developed a rapid and simple PCR method for detecting and identifying Azospirillum isolates within populations of rhizosphere bacteria. Significance and Impact of the Study: The method developed would serve as a useful tool for isolating a variety of indigenous Azospirillum bacteria from agricultural samples.

show abstract

Section: Correspondencementioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

A rapid and simple PCR method for identifying isolates of the genus Azospirillum within populations of rhizosphere bacteria

Shime-Hattori

Kobayashi

Ikeda³

et al. 2011

Journal of Applied Microbiology

View full text Add to dashboard Cite

show abstract

“…The advantage of the recent sequencing and DNA finger printing technology has facilitated the detection and identification of target microbes in the process of isolation in selective media and therefore reduced the morphophysiological tests (Stead et al, 2000). The use of techniques for detection, identification and quantification of microorganisms based on PCR can be completed by a dependent culture and biochemical methods.…”

Section: Introductionmentioning

confidence: 99%

Phenotypic and molecular characterization of plant growth promoting Rhizobacteria isolated from the rhizosphere of wheat (Triticum durum Desf.) in Algeria

Mahbouba¹,

Christine²,

Belbekri³

et al. 2013

Afr. J. Microbiol. Res.

View full text Add to dashboard Cite

Several strains were isolated from rhizosphere of wheat in Eastern Algeria. All the 10 strains (Azo4, Azo5, Azo6, Azo7, Azo8, Azo10, S1, S2, S3 and S9) were identified; based also on the characteristics which are not only morphological, characteristics such as shape but physiological ones: optimal temperature, pH optimum and NaCl tolerance. These isolates were screened in vitro for their growth promoting traits like production of indole acetic acid (IAA), ammonia (NH 3 ) and solubilize phosphate. The bacterial strains have an optimum growth pH equal to 6.8; in a broad temperature range (28 and 37°C). The molecular identification was done by simple PCR to amplification for 16S rDNA gene using primers AZ16S-D. Some strains (Azo4, Azo5, Azo6, Azo7, Azo8 and Azo10) have high identity with genus Azospirillum which indicates that these isolates belong to the Azospirillum genus, specially to species of Azospirillum brasilense A nested PCR approach performed with degenerate primers was used to amplify nifH gene fragments from the bulk DNA. The amplification product bands at the expected nifH gene fragment size are about 370 bp. Some isolates (S1, S2, S3, Azo4, Azo5, Azo6, S9 and Azo10) were considered by the nitrogen-fixing gene nifH detection of their genome. We managed to highlight the contribution between A. brasilense and durum wheat with inoculation experiments performed under greenhouse conditions showed that all A. brasilense (six strains) have a good report for growth of roots and plants and improve the production of durum wheat (Triticum durum var: GTA). These data demonstrate the importance of the test especially in programs to improve the quality of wheat in Algeria.

show abstract

“…Fatty acid profiles obtained were typical of P. syringae . REP‐PCR based on primers from the repetitive enterobacterial consensus sequence and BOX‐PCR, based on primers from the BOX A subunit, were carried out (Stead et al ., 2000). Fingerprints were compared with those of reference strains of all validly named P. syringae pathovars held in the NCPPB, including P.s .…”

mentioning

confidence: 99%

First record of Pseudomonas syringae pv. viburni in the UK

Stead¹,

Stanford²,

Aspin³

et al. 2006

Plant Pathology

View full text Add to dashboard Cite

Modern Methods for Characterising, Identifying and Detecting Bacteria Associated With Plants

Cited by 14 publications

References 0 publications

A rapid and simple PCR method for identifying isolates of the genus Azospirillum within populations of rhizosphere bacteria

A rapid and simple PCR method for identifying isolates of the genus Azospirillum within populations of rhizosphere bacteria

Phenotypic and molecular characterization of plant growth promoting Rhizobacteria isolated from the rhizosphere of wheat (Triticum durum Desf.) in Algeria

First record of Pseudomonas syringae pv. viburni in the UK

Contact Info

Product

Resources

About