Modern Methods of Aptamer Chemical Modification And Principles of Aptamer Library Selection

Abstract

Aptamers are promising molecules for a wide range of applications in biomedicine and various diagnostic systems due to their unique properties as selective ligands, speci cally obtained for a selected target using methods of arti cial evolution and combinatorial chemistry. We discuss strategies of obtaining aptamers in vitro and using their chemical modi cations, as well as approaches to design initial compound libraries based on in silico prestructuring. Limitations are formulated, and a direction for the development of the eld in obtaining new aptamers is proposed.