Modern Methods of Aptamer Chemical Modification and Principles of Aptamer Library Selection

Subach, M. F.; Khrenova, M. G.; Zvereva, M. I.

doi:10.3103/s002713142470010x

Cited by 1 publication

(3 citation statements)

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“…However, they are not widely used in the development of potential drugs since such a substitution results in the nonspecific binding to partially complementary DNA as well as to proteins. Most often, phosphorodithioate oligonucleotides are used in the design of aptamer libraries [34].…”

Section: Substitution Of Non-bridging Oxygen Atomsmentioning

confidence: 99%

“…complementary DNA as well as to proteins. Most often, phosphorodithioate oligonucleotides are used in the design of aptamer libraries [34]. Another significant modification is the substitution of oxygen with carbon (7), primarily the methyl group (R = H) [35].…”

Section: Substitution Of Non-bridging Oxygen Atomsmentioning

confidence: 99%

“…The increased affinity of formacetal oligonucleotides to complementary RNA fragments can be further increased by substituting the 3 ′ -bridging oxygen with a sulfur atom when forming a thioacetal linkage (28) [73]. Based on the replacement of the phosphorus atom with a sulfur atom, a variety of modified oligonucleotides with sulfamate (29, 30) [74,75], sulfamide (31) [76,77], sulfonamide (32) [78], sulfonic (33), and dimethylene sulfide (34) bridging groups [79], often having interesting characteristics, have been obtained. However, when creating modified polymers, amide fragments (35)(36)(37)(38)(39)(40) are most often used as alternative acyclic linkages, allowing the internucleotide crosslinking to be configured in different ways [80][81][82][83].…”

Section: Acyclic Linkagesmentioning

confidence: 99%

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A Visual Compendium of Principal Modifications within the Nucleic Acid Sugar Phosphate Backbone

Novikova,

Sagaidak,

Vorona

et al. 2024

Molecules

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Nucleic acid chemistry is a huge research area that has received new impetus due to the recent explosive success of oligonucleotide therapy. In order for an oligonucleotide to become clinically effective, its monomeric parts are subjected to modifications. Although a large number of redesigned natural nucleic acids have been proposed in recent years, the vast majority of them are combinations of simple modifications proposed over the past 50 years. This review is devoted to the main modifications of the sugar phosphate backbone of natural nucleic acids known to date. Here, we propose a systematization of existing knowledge about modifications of nucleic acid monomers and an acceptable classification from the point of view of chemical logic. The visual representation is intended to inspire researchers to create a new type of modification or an original combination of known modifications that will produce unique oligonucleotides with valuable characteristics.

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Section: Substitution Of Non-bridging Oxygen Atomsmentioning

confidence: 99%

Section: Substitution Of Non-bridging Oxygen Atomsmentioning

confidence: 99%