Modern Possibilities for the Vireoretinal Interface Contrasting (Experimental Study)

Abstract

The contrast agents in vitreoretinal surgery are necessary to ensure the visualization of translucent and ultra-thin membranes and vitreoretinal interface (VRI) structures, but the ideal staining agent has not been found yet.Purpose. To investigate and compare staining properties of two vital dyes for vitreous body (VB) and vitreoretinal interface visualization: “Kenalog-40” and “Vitreocontrast”.Patients and methods. The study was performed on 20 cadaveric eyes. After eyeball dissection with the original technology, we carried out posterior vitreous cortex separation and comparative staining of retinal fragments on the side of VB and corresponding section of the VB by “Kenalog-40” and “Vitreokontrast”. Vitreo-retinal interface samples underwent morphological analysis.Results. In contrast to «Kenalog-40», «Vitreocontrast» enables the visualization of thin layer of VB on retina surface after splitting of cortical layers during posterior vitreous detachment induction. Upon that, the adherent particles of the “Vitreocontrast” suspension remained on the corresponding splitted fragment of the VB. After staining with “Kenalog-40”, the VB surface remained smooth, shiny, with no visible changes. The results of histological examination of the obtained retinal and VB samples confirmed the presence of VB layer on the retina surface at the site of vitreoshisis formation during the induction of posterior vitreous detachment.Conclutions. Received data confirm the possibility of vitreoshisis formation during posterior vitreous body detachment, and demonstrate the advantages of the staining properties of the “Vitreokontrast” suspension for visualization of fine structures of vitreoretinal interface.

Features of hemodynamic and immunological parameters in patients with recurrent uveitis complicated by hypertension, Fuchs heterochromic uveitis and Posner-Schlossman syndrome

“…In cases with proliferative process in the choroid, a significant decrease in the maximum and minimum blood flow velocities in the posterior short and long ciliary arteries was noted in comparison with the control group. This was possible by studying the state of regional hemodynamics using Doppler ultrasound of the eye vessels in chorioretinitis of various etiologies [ 34 ].…”

Perspective Chapter: The Vitreous Body Visualization Technique in Diagnosis and the Classification of Idiopathic Macular Holes

“…A number of researchers showed that modern vital dyes due to their nonselective diffusion stained not only VB but the surrounding intraocular structures too, which impaired the identification of VB. Besides, the abovementioned agents have a number of side effects (cytotoxic, phototoxic, cataractgenic action, elevated intraocular pressure, and pharmacological activity) [6,11,15,16].…”

“…New staining solutions provide an opportunity to study anatomic topographic changes in the structures of the vitreous body (VB) and vitreoretinal interface (VRI) during surgical intervention with possible subsequent histological and ultrastructural studies [1,10]. All this contributes to the development of innovative methods and modernization of existing approaches for the treatment of diseases of the posterior segment of the eyeball [4][5][6][11][12][13][14][15].…”

Anatomic and Topographic Vitreous and Vitreoretinal Interface Features in Proliferative Diabetic Vitreoretinopathy

“…Однако работ, представляющих данные интраоперационной картины данных изменений кортикальных слоев СТ и ВРИ, в настоящее время не представлено. В мировой литературе не описаны методики, позволяющие прижизненно контрастировать данные структуры как в норме, так и при патологических изменениях, в том числе при ПДР [11][12][13][14][15][16].…”