Modern TLC: A key technique for identification and quality control of botanicals and dietary supplements

Schibli, Anne; Reich, Eike

doi:10.1556/jpc.18.2005.1.6

Cited by 26 publications

(13 citation statements)

References 2 publications

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“…HPTLC fingerprints were generated for the RCM-107 formula and green tea samples, which were developed on silica gel using the mobile phase toluene, acetone, formic acid 9:9:2 (v/v/v) (Schibli and Reich, 2005). After derivatization with Fast Blue Salt B reagent (Schibli and Reich, 2005), all polyphenols were readily identified under white light. EGCG, ECG and EC with increasing Rf values (0.35, 0.46 and 0.54, respectively) were used as authentic standards (Fig.…”

Section: Resultsmentioning

confidence: 99%

“…Polyphenols (EGCG, ECG and EC): 2.0 mL of Fast Blue Salt B reagent was sprayed over the plate. Evaluation was performed under white light or UV 254 nm (Schibli and Reich, 2005). Flavonoids (quercetin, rutin and Q3 O G): 2.0 mL of NP reagent was sprayed over the plate, with a 5 min delay, then 2.0 mL of PEG solution was sprayed.…”

Section: Methodsmentioning

confidence: 99%

“…Flavonoids (quercetin, rutin and Q3 O G): 2.0 mL of NP reagent was sprayed over the plate, with a 5 min delay, then 2.0 mL of PEG solution was sprayed. Evaluation was performed under UV 366 nm (Schibli and Reich, 2005). Crocin: no derivatization was required.…”

Section: Methodsmentioning

confidence: 99%

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The inhibitory effects of an eight-herb formula (RCM-107) on pancreatic lipase: enzymatic, HPTLC profiling and in silico approaches

Luo

Gill

Dias

et al. 2019

Heliyon

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AimsObesity is a global, public health issue that causes or exacerbates serious medical disorders. Chinese herbal therapies have become one of the most popular alternatives due to intolerances of current anti-obesity treatments. The RCM-107 formula (granule) is modified from our previous studied RCM-104 formula, which has demonstrated significant effects on weight reduction in randomized clinical trials. Up to date, there is no published scientific evidence to evaluate the effect of this formula on the weight-loss target pancreatic lipase and therefore, the aim of this study is to investigate the inhibitory effect of RCM-107 and respective individual ingredient on the pancreatic lipase activities.Main methodsFluorometric based enzymatic assays, high-performance thin-layer chromatography (HPTLC) profiling and in silico molecular docking techniques were used to investigate the lipase inhibitory effects of the RCM-107 herbal formula and its respective individual herbs.Principle findingsThe results demonstrated the potent lipase suppressing effect of the RCM-107 formula. The majority of the ingredients from this formula also showed pancreatic lipase inhibitory activities. The presence of the known weight-loss compounds such as (-)-epigallocatechin-3-gallate (EGCG), epicatechin-3-gallate (ECG), (-)-epicatechin (EC), rutin, crocin and caffeine were identified in the RCM-107 and related single herbs using HPTLC profiling approaches. In addition, EGCG, EC and the known lipase antagonist orlistat acted on the same site. These compounds form hydrogen bonds with corresponding residues HIS152, ASP80 and GLY77, which can be considered as markers of important areas in the ligand-binding site. This may explain the details of their roles in inhibiting pancreatic lipase activities.ConclusionOur data has provided new knowledge to the mechanistic properties of the RCM-107 formula and its respective individual herbal ingredients for weight loss, in terms of reducing lipid absorption via the inhibition of pancreatic lipase.

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Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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The inhibitory effects of an eight-herb formula (RCM-107) on pancreatic lipase: enzymatic, HPTLC profiling and in silico approaches

Luo

Gill

Dias

et al. 2019

Heliyon

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“…Since its inception planar chromatography was always indispensable in plant analysis, be it as a rapid tool for comparison of column fractions, optimization of solvent mixtures for preparative column chromatography, identification of plant material, screening for certain biological activity or content of markers and others. It is not necessary to repeat all the general advantages inherent to the planar chromatographic process at this point [8], but the level of sophistication available today in HPTLC has raised the analytical quality of results significantly. Accepting the principal equivalence in quality, HPTLC can be viewed as an orthogonal technique to HPLC enabling a look at an analytical problem from a different angle for complementary information.…”

Section: Why Hptlc For Plant Analysis?mentioning

confidence: 99%

Plant Analysis 2008 – Planar Chromatography

Reich¹,

Widmer²

2008

Planta Med

Self Cite

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“…Taking the advantage of its low-cost, less dependent on expensive equipment, flexible mobile phase composition, easy post-derivatization and the intact images of the multiple herbal samples in parallel on the same plate for being instant comparative viewing. Therefore it is still keeping active and alive in herbal medicines quality control nowadays [11][12][13][14][15]. In addition to the two dimension parameters of migration distance (retention time for HPLC, R f value for HPTLC) and integration peaks area/height, the various visible or fluorescence images is uniquely the third dimension parameter for validation of the chromatograms.…”

Section: Introductionmentioning

confidence: 99%

Value the Unique Merit of HPTLC Image Analysis and Extending its Performance by Digitalization for Herbal Medicines Quality Control

Xie¹,

Sun²,

Xu³

et al. 2014

J Chromatogr Sep Tech

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It is well known that High-Performance Thin Layer-Chromatography (HPTLC), the off-line planar chromatographic technique, has been employed in the pharmacopoeias of many countries for identification by virtue of its low-cost, less dependent on expensive equipment, flexible mobile phase composition and easy post-derivatization. In particular, the unique merit of HPTLC is able to provide the picture-like images of multiple samples in parallel on the same plate for instant viewing. In addition to the two dimension parameters (migration distance (R f value), integration peak value), the attractive color image, as the third dimension parameter, enhances the potency of HPTLC identification. The bioactivity of herbal medicines released from its compound bioactive ingredients in a holistic manner. Therefore the detected total chemical composition in the image should be reasonably more meaningful to assessing the inherent quality than only any selected single marker constituent of the given herbal drug. A vivid colorful picture-like HPTLC image could be easily recognized but not described properly by words. Hence extending the plate spot/band capacity on the plate for involving the bioactive components as much as possible. Combining digitizing the HPTLC image, the acquired infographic (HPTLC image, the digital scanning profile and the integrated parameters) can be used for establishing the fingerprint common pattern of the given species. It can be coupled with chemometrics analysis for more effectively quantifiable assessing the inherit quality of the herbal drugs. In this paper we reported methodically some Chinese herbal drugs analysis via various levels for demonstration of the practical application in QC.

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Modern TLC: A key technique for identification and quality control of botanicals and dietary supplements

Cited by 26 publications

References 2 publications

The inhibitory effects of an eight-herb formula (RCM-107) on pancreatic lipase: enzymatic, HPTLC profiling and in silico approaches

The inhibitory effects of an eight-herb formula (RCM-107) on pancreatic lipase: enzymatic, HPTLC profiling and in silico approaches

Plant Analysis 2008 – Planar Chromatography

Value the Unique Merit of HPTLC Image Analysis and Extending its Performance by Digitalization for Herbal Medicines Quality Control

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