Modes of intercellular transcription factor movement in the<i>Arabidopsis</i>apex

Wu, Xuelin; Dinneny, José R.; Crawford, Katrina M.; Rhee, Yoon; Citovsky, Vitaly; Zambryski, Patricia; Weigel, Detlef

doi:10.1242/dev.00577

Cited by 211 publications

(174 citation statements)

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“…However, other NCATFs, like LFY have been suggested to move cell to cell via a diffusionbased non-selective mode in which the NCATF does not need a direct interaction with PD components (Wu et al, 2003). In this regard, mCherry is an example of non-selective/diffusionbased movement.…”

Section: Genome-wide Screening For Non-cell-autonomous Transcription mentioning

confidence: 99%

“…Other well-characterized NCATFs include the floral homeotic proteins, LEAFY (Sessions et al, 2000;Wu et al, 2003), AGAM-OUS (Urbanus et al, 2010), DEFICIENS and GLOBOSA (Perbal et al, 1996), as well as TFs involved in root development, such as the Arabidopsis GRAS family member, SHORT ROOT (SHR) (Nakajima et al, 2001), CAPRICE (CPC), a Myb-like DNA-binding domain protein (Kurata et al, 2005;Wada et al, 2002), basic helix-loop-helix proteins GLABRA 3 (GL3) and ENHANCED GLABRA 3 (EGL3) (Bernhardt et al, 2005).…”

Section: Introductionmentioning

confidence: 99%

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Analysis of Arabidopsis Transcription Factor Families Revealed Extensive Capacity for Cell-to-Cell Movement as Well as Discrete Trafficking Patterns

Rim

Huang

Chu

et al. 2011

Molecules and Cells

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In plants, cell-to-cell communication is pivotal for the orchestration of cell fate determination, organ development, and the integration of whole plant physiology. One of the strategies for intercellular communication uses symplasmic communication channels, called plasmodesmata (PD). These PD establish unique cytoplasmic channels for the intercellular exchange not only of metabolites and small signaling molecules, but also of regulatory proteins and RNAs to allow for local orchestration of development and physiology. A number of non-cell-autonomous transcription factors (NCATFs) have been shown to function in the coordination of specific regulatory networks. To further explore the potential of such NCATFs, a genome-wide screen was performed on the transcription factor (TF) families in Arabidopsis. We here report that, among the 76 TFs examined, 22 were shown to move beyond their sites of transcription in the root apex; these NCATFs belonged to 17 TF families, including homeobox, GRAS, and MYB. Expression studies performed on variously-sized mCherry constructs identified a range of PD size exclusion limits within tissues of the root. In addition, our studies showed that actual protein level was an important factor controlling the range of TF intercellular movement. Interestingly, our studies on CAPRICE movement revealed tissue-specificity with respect to the mode of intercellular trafficking. These findings are discussed with respect to the regulation between cell-autonomous or non-cell-autonomous action.

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Section: Genome-wide Screening For Non-cell-autonomous Transcription mentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Analysis of Arabidopsis Transcription Factor Families Revealed Extensive Capacity for Cell-to-Cell Movement as Well as Discrete Trafficking Patterns

Rim

Huang

Chu

et al. 2011

Molecules and Cells

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“…Because the interactions between DOT/UFO and ALF/LFY and the target promoters are direct, cell nonautonomy probably results from the intercellular movement of DOT/ UFO rather than that of a downstream factor. Given that DOT is not fully targeted to an intracellular compartment, it is likely to move between cells by passive diffusion, similar to LFY (Sessions et al, 2000;Wu et al, 2003), which would result in a DOT gradient within the flower (Figure 8). Weak fim and dot alleles primarily affect petal development (Ingram et al, 1997) (Figure 1), suggesting that the activation of B genes requires higher DOT and FIM activity than the activation of C genes.…”

Section: Role Of Ufo and Dot In Flower Developmentmentioning

confidence: 99%

“…Because this effect of DOT requires ALF ( Figure 6A), it appears that ALF expression in the apical FM can alter the identity of the lateral IM in a non-cellautonomous manner. It was previously shown that the cell nonautonomy of LFY activity is associated with the movement of the protein between meristem cells (Sessions et al, 2000;Wu et al, 2003). Thus, it is the transcription pattern of DOT in the apex, rather than that of ALF, that restricts floral identity to the apical meristem and specifies the cymose architecture.…”

Section: Evolution and Development Of Distinct Inflorescencesmentioning

confidence: 99%

Patterning of Inflorescences and Flowers by the F-Box Protein DOUBLE TOP and the LEAFY Homolog ABERRANT LEAF AND FLOWER of Petunia

et al. 2008

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Angiosperms display a wide variety of inflorescence architectures differing in the positions where flowers or branches arise. The expression of floral meristem identity (FMI) genes determines when and where flowers are formed. In Arabidopsis thaliana, this is regulated via transcription of LEAFY (LFY), which encodes a transcription factor that promotes FMI. We found that this is regulated in petunia (Petunia hybrida) via transcription of a distinct gene, DOUBLE TOP (DOT), a homolog of UNUSUAL FLORAL ORGANS (UFO) from Arabidopsis. Mutation of DOT or its tomato (Solanum lycopersicum) homolog ANANTHA abolishes FMI. Ubiquitous expression of DOT or UFO in petunia causes very early flowering and transforms the inflorescence into a solitary flower and leaves into petals. Ectopic expression of DOT or UFO together with LFY or its homolog ABERRANT LEAF AND FLOWER (ALF) in petunia seedlings activates genes required for identity or outgrowth of organ primordia. DOT interacts physically with ALF, suggesting that it activates ALF by a posttranslational mechanism. Our findings suggest a wider role than previously thought for DOT and UFO in the patterning of flowers and indicate that the different roles of LFY and UFO homologs in the spatiotemporal control of floral identity in distinct species result from their divergent expression patterns.

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“…Wu et al (2003) suggested that cell-to-cell movement of transcription factors results from free diffusion in a non-targeted fashion (Wu et al, 2003). On the other hand, Sessions et al (2000) showed that two independent transcription factors can traffic long and short ranges in a targeted fashion.…”

Section: Discussionmentioning

confidence: 99%

Different subcellular localization and trafficking properties of KNOX class 1 homeodomain proteins from rice

et al. 2004

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Genes of the KN1-like homeobox (KNOX) class 1 encode transcription factors involved in shoot apical meristem development and maintenance. We studied the subcellular localization of Green Fluorescent Protein-tagged rice KNOX proteins (Oskn1-3) after particle bombardment of onion and rice cells and after transformation of Arabidopsis and rice with constitutive and inducible expression constructs. In all test systems, the three rice KNOX proteins showed nuclear and cytoplasmic localization patterns. However, Oskn1 additionally showed in some cells a distribution over punctae moving randomly in the cytosol. Use of an inducible expression system indicated a nuclear presence of Oskn1 in cells of the shoot apical meristem and post-transcriptional down-regulation in early leaf primordia. Arabidopsis and rice test systems were used to study effects of plant hormones and auxin transport inhibition on KNOX protein localization. Application of GA 3 or 1-NAA shifted protein localization completely to the cytoplasm and resulted in loss of the punctae formed by Oskn1. Conversely, NPA application induced a complete nuclear localization of the KNOX proteins. To study intercellular movement of the KNOX proteins we set up a novel co-bombardment assay in which trafficking of untagged KNOX proteins was visualized through the co-trafficking of green fluorescent or blue fluorescent marker proteins. In multiple independent experiments Oskn1 trafficked more extensively to neighboring cells than Oskn2 and Oskn3. Differences in the localization and trafficking properties of Oskn1, Oskn2 and Oskn3 correlate with differences in mRNA localization patterns and functional differences between the rice KNOX genes and their putative orthologues from other species.

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Modes of intercellular transcription factor movement in theArabidopsisapex

Cited by 211 publications

References 50 publications

Analysis of Arabidopsis Transcription Factor Families Revealed Extensive Capacity for Cell-to-Cell Movement as Well as Discrete Trafficking Patterns

Analysis of Arabidopsis Transcription Factor Families Revealed Extensive Capacity for Cell-to-Cell Movement as Well as Discrete Trafficking Patterns

Patterning of Inflorescences and Flowers by the F-Box Protein DOUBLE TOP and the LEAFY Homolog ABERRANT LEAF AND FLOWER of Petunia

Different subcellular localization and trafficking properties of KNOX class 1 homeodomain proteins from rice

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