Modest effect of temperature on the porcine oxygen dissociation curve

Willford, David C.; Hill, Elizabeth E.

doi:10.1016/0034-5687(86)90035-6

Cited by 47 publications

(18 citation statements)

References 22 publications

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“…This conclusion is supported by our findings of larger P 50 values in pig and sheep than in harbor porpoise, using the same experimental method on all species (Fig.1). The O 2 affinity of pig and sheep blood resembles earlier reports (Willford and Hill, 1986;Moraga et al, 1996), whereas the oxygen affinity of harbor porpoise blood is comparable to that in bottlenose dolphin and common dolphin (Kooyman, 1989). A relatively high blood O 2 affinity in the harbor porpoise is advantageous for effective O 2 extraction from the pulmonary O 2 store during dives (Snyder, 1983), as recently documented in diving emperor penguins ).…”

Section: Results and Discussion Blood Oxygen Transporting Propertiessupporting

confidence: 78%

Respiratory properties of blood in the harbor porpoise,Phocoena phocoena

Soegaard

Hansen

Elk

et al. 2012

Journal of Experimental Biology

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SUMMARYHarbor porpoises are active divers that exchange O 2 and CO 2 with the environment during a fast single breath upon surfacing. We investigated blood O 2 -transporting properties, buffer characteristics, Cl -transport via the erythrocyte anion exchanger (AE1), circulating nitric oxide metabolites and hemoglobin nitrite reduction in harbor porpoises with the aim to evaluate traits that are adaptive for diving behavior. Blood O 2 affinity was higher in harbor porpoises than in similar sized terrestrial mammals, as supported by our parallel recordings of O 2 equilibria in sheep and pig blood. Further, O 2 affinity tended to increase with increasing body mass. A high O 2 affinity favors O 2 extraction from the lungs, but a normal Bohr effect (logP 50 /pH-0.46) gradually lowers O 2 affinity during dives (where CO 2 accumulates) to assist O 2 off-loading to perfused tissues. The true plasma non-bicarbonate buffer value was moderately higher than in terrestrial mammals and increased upon deoxygenation. Plasma bicarbonate was also relatively high, contributing to increase the overall buffer capacity. The apparent Cl -permeability of harbor porpoise erythrocytes was similar to the human value at 37°C, showing absence of a comparative increase in the velocity of erythrocyte HCO 3 -/Cl -exchange to aid CO 2 excretion. The Q 10 for AE1-mediated Cl -transport in harbor porpoises was lower than in humans and seemed to match the Q 10 for metabolism (Q 10 Ϸ2). Plasma nitrite, plasma nitrate and hemoglobin-mediated nitrite reduction were elevated compared with mammalian standards, suggesting that increased nitric oxide bioavailability and nitrite-derived nitric oxide could play important roles in diving physiology.

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Section: Results and Discussion Blood Oxygen Transporting Propertiessupporting

confidence: 78%

Respiratory properties of blood in the harbor porpoise,Phocoena phocoena

Soegaard

Hansen

Elk

et al. 2012

Journal of Experimental Biology

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“…However, the O 2 -binding properties of Hb E at lower temperatures, more representative of O. mykiss's typical physiological temperatures, are unknown. Decreased temperature is known to lower the P 50 and n H and to raise the Bohr effect in Hb A (Irving et al, 1941;Cameron, 1971;Eddy, 1971;Weber et al, 1976;Vorger, 1985;Willford and Hill, 1986). Whether a lower temperature would have a similar effect in Hb E has yet to be investigated.…”

Section: Introductionmentioning

confidence: 99%

Hypoxia delays hematopoiesis: retention of embryonic hemoglobin and erythrocytes in larval rainbow trout,Oncorhynchus mykiss, during chronic hypoxia exposure

Bianchini

Wright

2013

Journal of Experimental Biology

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SUMMARYIn rainbow trout development, a switch occurs from high-affinity embryonic hemoglobin (Hb) and round, embryonic erythrocytes to lower-affinity adult Hb and oval, adult erythrocytes. Our study investigated the early ontogeny of rainbow trout blood properties and the hypoxia response. We hypothesized that hypoxia exposure would delay the ontogenetic turnover of Hb and erythrocytes because retention of high-affinity embryonic Hb would facilitate oxygen loading. To test this hypothesis we developed a method of efficiently extracting blood from individual embryos and larvae and optimized several techniques for measuring hematological parameters on microliter (0.5-2.0 μl) blood samples. In chronic hypoxia (30% of oxygen saturation), stage-matched embryos and larvae possessed half the Hb concentration, erythrocyte counts and hematocrit observed in normoxia. Hypoxia-reared larvae also had threefold to sixfold higher mRNA expression of the embryonic Hb α-1, β-1 and β-2 subunits relative to stage-matched normoxia-reared larvae. Furthermore, in hypoxia, the round embryonic erythrocytic shape persisted into later developmental stages. Despite these differences, Hb-oxygen affinity (P 50 ), cooperativity and the Root effect were unaltered in hypoxia-reared O. mykiss. The data support our hypothesis that chronic hypoxia delays the ontogenetic turnover of Hb and erythrocytes, but without the predicted functional consequences (i.e. higher than expected P 50 ). These results also suggest that the Hb-oxygen affinity is protected during development in chronic hypoxia to favor oxygen unloading at the tissues. We conclude that in early trout development, the blood-oxygen transport system responds very differently to chronic hypoxia relative to adults, possibly because respiration depends relatively more on oxygen diffusion than convection.

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“…Another important event that occurs during the transition HOS-LOS of the RBC is the change of the intracellular concentration of free 2,3-DPG. In this respect, it may be worthwhile to outline that the intraerythrocytic concentration of this organic phosphate in pig can be up to about 10 mM and that 2,3-DPG interacts with the erythrocyte membrane and with B3 in particular [1] [2] [18] [36]. Furthermore, 2,3-DPG causes an increase in the lateral mobility of integral membrane proteins [37] and, at concentrations which are normally present in erythrocytes, can weaken associations in the shell [38].…”

Section: Discussionmentioning

confidence: 99%

“…Pig RBC diameters and counts are similar, although the average life span of pig erythrocytes is shorter than that of human ones. The knowledge acquired to date on porcine blood shows that it is characterized by a high intraerythrocyte concentration of 2,3-diphosphoglycerate (2,3-DPG) (8 -10 mM) [1] [2] and the hemoglobin (Hb) shows a low temperature sensitivity. In addition, pig RBCs have the slowest metabolic rate of any mammalian cell type [3]; they contain both hexokinase (EC 2.7.1.1) types II and III, the latter being the predominant form accounting almost for 98% of the total glucose phosphorylating activity [4].…”

Section: Introductionmentioning

confidence: 99%

Metabolic Effects of Endogenous and Exogenous Heterotropic Hemoglobin Modulators on Anion Transport: The Case of Pig Erythrocytes

Tellone

Russo²,

Giardina³

et al. 2015

OALib

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This comparative study, focused on the anion kinetic of band 3 protein of pig erythrocytes, investigates in vitro the metabolic functionality of the red blood cells of this mammal which show particular characteristics of the cell membrane and significant stoichiometric variations of several cytosolic components. Pig red blood cells compared to human ones, are characterized by a higher anion flux strongly modulated by the conformational transition state (T-R) of hemoglobin; these findings are probably connected to the intracellular concentration of 2,3-disphosphoglycerate and to chloride activity. The experimental use, alone or in combination, of some exogenous heterotropic modulators of hemoglobin as orthovanadate, gemfibrozil and resveratrol, shows characteristic modulations of the anion flux which are related to the cytosolic metabolites physiologically present in pig erythrocytes, to the metabolism and oxidative stress. Our findings highlight new features about the pig red blood cells functionality and contribute to extending the current understanding of their life cycle, providing opportunities for new investigations in the field of comparative physiology.

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Modest effect of temperature on the porcine oxygen dissociation curve

Cited by 47 publications

References 22 publications

Respiratory properties of blood in the harbor porpoise,Phocoena phocoena

Respiratory properties of blood in the harbor porpoise,Phocoena phocoena

Hypoxia delays hematopoiesis: retention of embryonic hemoglobin and erythrocytes in larval rainbow trout,Oncorhynchus mykiss, during chronic hypoxia exposure

Metabolic Effects of Endogenous and Exogenous Heterotropic Hemoglobin Modulators on Anion Transport: The Case of Pig Erythrocytes

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