Modification des isozymes de la lacticodesliydrogénase musculaire au cours de l’atrophie expérimentale

Abstract

Les isozymes de la lacticodeshydrogénase (LDH) des muscles squelettiques d’embryon de poulet, et de foetus de cobaye, ont été comparés aux isozymes de la LDH de gastrocnémiens, adultes, normaux et atrophiés par dénervation, de ces mêmes espèces. Lorsque le type isozymique foetal diffère peu du type isozymique adulte (cas du cobaye), on ne trouve pas de différence des isozymes entre le muscle normal et le muscle atrophié. Par contre, dans le cas du poulet où le type embryonnaire de ces isozymes est très différent du type adulte, les auteurs mettent en évidence une modification de la répartition des isozymes qui rappelle à la fois l’état embryonnaire, et la dystrophie musculaire primitive.

Enzyme Studies in Muscular Dystrophy VI

“…In such dystrophic muscle the enzymatic activities of DNAse II in young adult [16] and cathepsin and other acid hydrolases in old chickens [14] are markedly increased. Lactic acid dehydrogenase is decreased, and the isozyme composition is shifted in the direction of the em bryonic type of this enzyme [10,12]. Herrmann et al [7] have reported that the deoxyribonucleic acid content of the muscle is also in creased in the young dystrophic chicken.…”

“…Herrmann et al [7] have reported that the deoxyribonucleic acid content of the muscle is also in creased in the young dystrophic chicken. Some of these investigations lead to the suggestion that hereditary dystrophy in the chicken results from a failure of muscle to differentiate fully [7,10,12]. How ever, in hereditary dystrophy in the mouse, it has been proposed that * * Supported by grants from Muscular Dystrophy Associations of America, Inc., and the U. S. Public Health Service.…”

Fetal-like Patterns of Lactic-Dehydrogenase and Aldolase Isozymes in some Pathological Conditions

“…We have shown along with Wieme and Herpol [25] that muscles of children afflicted with muscular dystrophy of Duchenne type possess this anodic pattern of lactic-dehydrogenase isozymes. The figure shows that the dystrophic muscles ressemble the fetal muscles (Fig.…”

“…We have found that in the species where the fetal type is different form the adult one, there are modifications of the isozymic pattern in muscular diseases [25,26]. We have shown with Jean Demos, in 1962, that the human fetal muscle is resolved by electrophoresis into princi pally anodic bands [9].…”

“…One of us has studied the two aldolase activities in muscles of rabbits, seven weeks after sciatic section [27]. The two aldolase activities (towards F-1-6-P and towards F-l-P) were determined separately in gastrocnemius and in soleus because the level of aldolase activity is about five times greater in the white than in the red muscle.…”