Modification of a Differential-Refractometer-Detector for Liquid Chromatography

Deininger, G.; Halász, I.

doi:10.1093/chromsci/8.9.499

Cited by 21 publications

(7 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Furthermore, the minimum sample detection limit is 3 × 10 -9 g/s, whereas that of the UV detector is 2 × 10 -11 g/s (Munk, 1970). Deininger and Halasz (1970) suggested that the sensitivity of the RI detector can be improved by using a heat exchanger through which the mobile phase flows before entering the cell. The above results suggest that these peaks in the glycolipid fraction of round frigate mackerel muscle lipids and sea snake crude fat are neutral glycosphingolipid and glycoglycerolipid.…”

Section: Resultsmentioning

confidence: 99%

Analyses of Glycolipids from Fish, Shellfish, and Sea Snake Lipids by High-Performance Liquid Chromatography

Lim¹,

Park²,

Suzuki³

1999

J. Agric. Food Chem.

View full text Add to dashboard Cite

To determine the existence of glycolipids (neutral glycosphingolipid and glycoglycerolipid) in sea snake, round frigate mackerel, sardine, sea urchin, and abalone, we performed silica gel chromatography and high-performance liquid chromatography (HPLC) using an Aquasil-SS column and a C(8)-reversed phase silica gel column. HPLC with a UV absorption detector was used to analyze neutral glycosphingolipid. These chromatograms showed typical peaks in round frigate mackerel lipid, in sea snake crude fat, in abalone intestine lipid, and in sea urchin intestine lipid. UV-HPLC was also used to analyze glycoglycerolipid. These chromatograms indicated a large peak in round frigate mackerel lipid and a small peak in purified sardine oil. In addition, we observed the same peaks in the glycolipid fraction of round frigate mackerel muscle lipids and sea snake crude fat using a differential refractometer detector. The results of this study suggest that the peaks are neutral glycosphingolipid or glycoglycerolipid and that neutral glycosphingolipid and glycoglycerolipid may have specific physiological functions in each living creature.

show abstract

Section: Resultsmentioning

confidence: 99%

Analyses of Glycolipids from Fish, Shellfish, and Sea Snake Lipids by High-Performance Liquid Chromatography

Lim¹,

Park²,

Suzuki³

1999

J. Agric. Food Chem.

View full text Add to dashboard Cite

show abstract

“…Notable modifications include the elimination of the need to operate at elevated temperatures; 8 incorporation of a second column and pumping system; 9 continuous alteration of the laser interrogation angle to adjust for RI changes in MP composition to allow for gradient separations; 10 and the use of thermooptic 11 and interferometric 12–14 methods to increase sensitivity. To date, however, refractometer design modifications have yet to result in significant adoption.…”

Section: Introductionmentioning

confidence: 99%

mentioning

confidence: 99%

“…In the decades following the introduction of RI sensing to the commercial LC detection landscape, there have been multiple attempts to improve upon the original design to increase its overall applicability. Notable modifications include the elimination of the need to operate at elevated temperatures, 8 incorporation of a second column and pumping system, 9 continuous alteration of the laser interrogation angle to adjust for RI changes in MP composition to allow for gradient separations, 10 and the use of thermooptic 11 and interferometric 12−14 Surface plasmon resonance (SPR), a technology most commonly utilized as a surface sensitive biosensor, has previously been interfaced with LC as a detector for oligosaccharide, 15 protein, 16,17 and carbohydrate 18−20 analysis. The most notable advantages of SPR over traditional RI detectors for LC are the speed at which the detector can achieve a stable baseline (50 min versus >2 h for commercial detectors 19 ) and the modification of sensor surface to enhance sensitivity.…”

mentioning

confidence: 99%

See 1 more Smart Citation

Refractive Index-Based Detection of Gradient Elution Liquid Chromatography using Chip-Integrated Microring Resonator Arrays

Wade

Bailey

2013

Anal. Chem.

View full text Add to dashboard Cite

Refractive index-based sensors offer attractive characteristics as non-destructive and universal detectors for liquid chromatographic separations, but a small dynamic range and sensitivity to minor thermal perturbations limit the utility of commercial RI detectors for many potential applications, especially those requiring the use of gradient elutions. As such, RI detectors find use almost exclusively in sample abundant, isocratic separations when interfaced with HPLC. Silicon photonic microring resonators are refractive index-sensitive optical devices that feature good sensitivity and tremendous dynamic range. The large dynamic range of microring resonators allows the sensors to function across a wide spectrum of refractive indices, such as that encountered when moving from an aqueous to organic mobile phase during a gradient elution – a key analytical advantage not supported in commercial RI detectors. Microrings are easily configured into sensor arrays, and chip-integrated control microrings enable real-time corrections of thermal drift. Thermal controls allow for analyses at any temperature and in the absence of rigorous temperature control, obviating extended detector equilibration wait times. Herein, proof of concept isocratic and gradient elution separations were performed using well characterized model analytes (e.g., caffeine, ibuprofen) in both neat buffer and more complex sample matrices. These experiments demonstrate the ability of microring arrays to perform isocratic and gradient elutions under ambient conditions, avoiding two major limitations of commercial RI-based detectors and maintaining comparable bulk RI sensitivity. Further benefit may be realized in the future through selective surface functionalization to impart degrees of post-column (bio)molecular specificity at the detection phase of a separation. The chip-based and microscale nature of microring resonators also makes it an attractive potential detection technology that could be integrated within lab-on-a-chip and microfluidic separation devices.

show abstract

“…The type of flow through a curved tube was widely employed in the design of chemical reactors and heat exchangers (Deininger and Halász 1970). The fluid flowing through a curved tube induces a different flow, which in turn enhances mixing of the fluid and increases heat and mass transfer rates (Dravid et al 1971;Naphon 2007).…”

Section: Introductionmentioning

confidence: 99%

Evaluation of Helically Coiled and Knitted Open Tubular Reactors for the Efficient Post-Column Determination of Tetrodotoxin by High-Performance Liquid Chromatography

et al. 2016

View full text Add to dashboard Cite

A post-column fluorescence reaction system for the high-performance liquid chromatographic (HPLC) determination of tetrodotoxin in the silver-cheeked toadfish Lagocephalus sceleratus is discussed theoretically and investigated experimentally. Ion-pair chromatography with volatile ammonium perfluoroheptanoate was used for the separation of tetrodotoxin and 4, 9-anhydrotetrodotoxin. The post-column reaction was based on tetrodotoxin conversion to a quinazoline fluorescent compound in strong alkaline conditions. All post-column parameters were optimized that affected the sensitivity, dispersion, and stability. Helically coiled and knitted open tubular reactors composed of polyetheretherketone were constructed and characterized in detail. The performance of these reactors was evaluated on the bases of sensitivity and dispersion. Their optimal design is reported. The knitted reactors were more efficient than the relevant helically coiled reactors when higher reaction times are required. A 1,500 μL polyetheretherketone knitted coil with 0.010″ internal diameter was optimum exhibiting higher pressure tolerances than Teflon coils. The HPLC post-column reaction method was evaluated in Downloaded by [University of Arizona] at 09:01 08 June 20163 terms of linearity, sensitivity, accuracy, precision, and ruggedness. The linear dynamic ranges for tetrodotoxin and 4, 9-anhydrotetrodotoxin were 40 to 3,000 ppb and 80 to 3,000 ppb, respectively. The limits of detection and quantification were 12 and 41 ppb for tetrodotoxin and 26 and 85 ppb for 4, 9-anhydrotetrodotoxin. The accuracy was evaluated by recovery measurements and the values for tetrodotoxin were between 90.7% and 93.6%. The use of a volatile perfuorocarboxylic acid as an ion-pair reagent allowed confirmation of by highperformance liquid chromatography -tandem mass spectrometry using identical mobile phase conditions.

show abstract

Modification of a Differential-Refractometer-Detector for Liquid Chromatography

Cited by 21 publications

References 0 publications

Analyses of Glycolipids from Fish, Shellfish, and Sea Snake Lipids by High-Performance Liquid Chromatography

Analyses of Glycolipids from Fish, Shellfish, and Sea Snake Lipids by High-Performance Liquid Chromatography

Refractive Index-Based Detection of Gradient Elution Liquid Chromatography using Chip-Integrated Microring Resonator Arrays

Evaluation of Helically Coiled and Knitted Open Tubular Reactors for the Efficient Post-Column Determination of Tetrodotoxin by High-Performance Liquid Chromatography

Contact Info

Product

Resources

About