Modification of allergen-induced airway obstruction and airway hyperresponsiveness in an allergic rabbit model by the selective platelet-activating factor antagonist, BN 52021

Coyle, AJ; Page, C. P.; Atkinson, Linda E.; Sjoerdsma, K; Touvay, Caroline; Wj, Metzger

doi:10.1016/0091-6749(89)90395-3

Cited by 50 publications

(22 citation statements)

References 30 publications

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“…Immunisation of neonatal NZW rabbits was performed by modifying (Coyle et al, 1989) a previously described method (Shampain et al, 1982). Allergen -immunised rabbits were injected intraperitoneally (0.5 ml) within 24 h of birth with Alternaria tenuis extract (40,000 pnu ml-') in aluminium hydroxide (Al(OH)3) moist gel (10 mg) and 0.9% sterile saline in the ratio 2:1:1.…”

Section: Immunisation Protocolsmentioning

confidence: 99%

Characterization of adenosine receptors involved in adenosine‐induced bronchoconstriction in allergic rabbits

El-Hashim

D’Agostino

Matera

et al. 1996

British J Pharmacology

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1 Recent work has suggested that adenosine may be involved in asthma via the activation of A1 receptors. However, the role of the recently cloned A3 receptor in airways is largely unknown. In the present study, we have investigated the role of the A3 receptor in adenosine-induced bronchoconstriction in allergic rabbits. 2 Aerosol challenge of antigen (Ag) immunized rabbits with the adenosine precursor, adenosine 5'-monophosphate (AMP), resulted in a dose-dependent fall in dynamic compliance (Cdyn). The maximum fall in Cdyn in these rabbits was significantly greater than that in litter matched, sham immunized animals (P<0.05). However, there was no significant difference in the maximum increase in airways resistance (RL) between Ag and sham immunized rabbits (P>0.05).3 Aerosol challenge of Ag immunized rabbits with cyclopentyl-adenosine (CPA) (Al-receptor agonist) elicited a dose-dependent fall in Cdyn in Ag immunized rabbits and the maximum fall in Cdyn in these rabbits was significantly greater than that observed in sham immunized rabbits (P<0.05). Similarly, CPA induced dose-dependent increases in RL in Ag immunized rabbits whereas sham immunized rabbits failed to respond to CPA within the same dose range. The maximum increase in RL in Ag immunized rabbits was significantly greater than that of sham immunized rabbits (P<0.05). 4 Aerosol challenge of either Ag or sham immunized rabbits with the A3 agonist aminophenylethyladenosine (APNEA) did not elicit dose-dependent changes in either RL or Cdyn. Moreover, there was no significant difference in the maximum response, measured by either parameter, between the two animal groups (P>0.05).5 These data provide further evidence for a role of the Al receptor in the airways, but do not support a role for the A3 receptor in adenosine-induced bronchoconstriction in the allergic rabbit.

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Section: Immunisation Protocolsmentioning

confidence: 99%

Characterization of adenosine receptors involved in adenosine‐induced bronchoconstriction in allergic rabbits

El-Hashim

D’Agostino

Matera

et al. 1996

British J Pharmacology

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“…The extracts of Ginkgo biloba mainly contain flavonoid glycosides and terpenoids (ginkgolide, bilobalide) . Ginkgolides have been demonstrated to function as antagonists of platelet-activating factor (Lamant et al, 1987), exhibit a wide range of pharmacological actions, such as inhibiting the aggregation of platelet (Akiba et al, 1998), arterial thrombosis (Bourgain et al, 1987), acute infl ammation (Teather et al, 2002), allergic reaction (Coyle et al, 1989) and so on. Ginkgolides in the terpeniod extracts can be divided into isotypes A, B, C, M, and J among which Ginkgolide B (GB) (C 20 H 24 O 10 ) has the highest biological activity (Li et al, 2009).…”

Section: Introductionmentioning

confidence: 99%

Ginkgolide B promotes proliferation and functional activities of bone marrow-derived endothelial progenitor cells: involvement of Akt/eNOS and MAPK/p38 signaling pathways

Tang

Huang²,

Sun³

et al. 2011

eCM

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Bone marrow-derived, circulating endothelial progenitor cells (EPCs) contribute to neovascularization in various diseases, and represent a very interesting alternative cell source for enhancing vasculogenesis in regenerative medicine. In this study, we investigated the effects of Ginkgolide B (GB) on proliferation and differentiation of EPCs, and the involved signaling pathway in vitro. EPC proliferation, migration, adhesion and angiogenesis activities were assessed with the WST-8 assay, Transwell chamber assay, cell counting and angiogenesis kit, respectively. Apoptosis was detected with annexin V and propidium iodide staining. The protein expression of angiogenesis-related makers was detected by Western blot, and related gene expression was determined by real-time polymerase chain reaction (RT-PCR). The results showed that GB promoted the proliferation and endothelial gene expression, and markedly enhanced vascular endothelial growth factor-induced migration response and the capability to incorporate into the vascular networks in EPCs. GB protected EPCs from H 2 O 2 -induced cell death. GB induced the phosphorylation of eNOS, Akt and p38, which in turn promoted cell proliferation and function. In conclusion, the present study demonstrates that GB, at a near medical applied dose, increases the number and functional activities of EPCs with involvement of Akt/endothelial nitric oxide synthase and mitogen-activated protein kinase (MAPK)/ p38 signal pathways. These findings raise the intriguing possibility that GB may play an important role in the protection and revascularization of blood vessels.

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“…immunization protocol, SHAMPAIN et al [13] and others [14,15] have described how animals sensitized to either Alternaria tenuis or ragweed allergen undergo early and late airway responses to acute antigen challenge. These responses are attenuated by clinically useful anti-asthma drugs [16], and the animals develop 2-6 fold increases in acute airway responsiveness, which resolves in one week [14,15,17,18].…”

Section: Technical Notementioning

confidence: 99%

“…These responses are attenuated by clinically useful anti-asthma drugs [16], and the animals develop 2-6 fold increases in acute airway responsiveness, which resolves in one week [14,15,17,18]. Repeated measurements of lung function are readily made in the rabbit, and each animal may be used as its own control [18,19].…”

Section: Technical Notementioning

confidence: 99%

A method for the long-term exposure of rabbits to environmental pollutant gases

Douglas

Price²,

Page³

1994

Eur Respir J

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A A m me et th ho od d f fo or r t th he e l lo on ng g--t te er rm m e ex xp po os su ur re e o of f r ra ab bb bi it ts s t to o e en nv vi ir ro on nm me en nt ta al l p po ol ll lu ut ta an nt t g ga as se es s ABSTRACT: The aims of the present study were twofold. Firstly, we wanted to develop a system for the exposure of rabbits to pollutant gases that would monitor gas concentrations accurately, allow flexibility, be simple to operate, and could be constructed at relatively modest cost. Additionally, we wanted to determine whether the procedures necessary for the daily exposure of young rabbits had any detrimental effect on their development. Using the environmental exposure system that we developed, littermate New Zealand White rabbits, neonatally immunized to either Alternaria tenuis or house dust mite antigen were exposed 2 h daily, from within 24 h of birth until 3 months of age, to either 4 ppm nitrogen dioxide (NO 2 ), or 5 ppm sulphur dioxide (SO 2 ) or ambient air.The environmental exposure system consists of four sections; a stainless steel exposure chamber; an airflow monitoring and control system and gas delivery system; a gas detector and monitoring system; and an exhaust fan. Equilibration and wash-out times of gas were short and the gas mixing within the chamber atmosphere was uniform. Levels of gases were reliably maintained throughout the period of exposure within predetermined limits. The weights of the immunized, gas-exposed animals did not differ significantly from those of the immunized, airexposed animals at any time throughout the 3 month period of exposure. At 3 months of age, the basal values for lung resistance and dynamic compliance did not differ between gas-and air-exposed rabbits. These values did not differ significantly from those obtained from naive animals of the same age.Our results suggest that we have developed a sensitive, reliable and simple environmental exposure and monitoring system. It is anticipated that the methodology described will allow the careful investigation of the effects of long-term exposure to pollutant gases from birth on the development of airways hyperresponsiveness.

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Modification of allergen-induced airway obstruction and airway hyperresponsiveness in an allergic rabbit model by the selective platelet-activating factor antagonist, BN 52021

Cited by 50 publications

References 30 publications

Characterization of adenosine receptors involved in adenosine‐induced bronchoconstriction in allergic rabbits

Characterization of adenosine receptors involved in adenosine‐induced bronchoconstriction in allergic rabbits

Ginkgolide B promotes proliferation and functional activities of bone marrow-derived endothelial progenitor cells: involvement of Akt/eNOS and MAPK/p38 signaling pathways

A method for the long-term exposure of rabbits to environmental pollutant gases

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