Modification of antigen expression in human and hamster pancreatic cancer cell lines induced by sodium butyrate

Corrà, Stefano; Kazakoff, Katherine; Mogaki, Masatoshi; Cano, Martín; Pour, Parviz M.

doi:10.1002/tcm.1770130502

Cited by 6 publications

(4 citation statements)

References 28 publications

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“…An upregulation of both NEP/CD10 mRNA and protein expression was found by 2 mM BA treatment of PATU‐8988T, HUP‐T3 and HUP‐T4 cells. Concentrations of 3 mM BA and higher induced an approximately 40% growth arrest of these cells, a result compatible with other investigations in pancreatic tumor cell lines 40, 41, 42, 43, 44, 45, 46. Expression profiling of BA‐treated lung carcinoma cells resulted in changes of genes related to cytokine signaling and cancer metastasis 47.…”

Section: Discussionsupporting

confidence: 88%

Expression of neutral endopeptidase (NEP/CD10) on pancreatic tumor cell lines, pancreatitis and pancreatic tumor tissues

Erhuma¹,

Köbel²,

Mustafa³

et al. 2007

Intl Journal of Cancer

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Neutral endopeptidase (NEP/CD10) is a cell surface zinc metalloprotease cleaving peptide bounds on the amino terminus of hydrophobic amino acids and inactivating multiple physiologically active peptides. Loss or decrease in NEP/CD10 expression have been reported in many types of malignancies, but the role of NEP/ CD10 in pancreatic carcinoma has not yet been identified. Using real-time RT-PCR, flow cytometry as well as immunohistochemistry, NEP/CD10 expression was quantified in both pancreatic carcinoma cell lines and in tumor specimens obtained from patients with primary pancreatic carcinomas. Three out of 8 pancreatic carcinoma cell lines exhibit heterogeneous NEP/CD10 expression levels: PATU-8988T expressed the highest NEP/CD10 levels, whereas HUP-T4 and HUP-T3 cells showed a moderate to low NEP/CD10 expression. NEP/CD10 immunoreactivity was found in 6 of 24 pancreatic ductal adenocarcinomas, but also in 3 of 6 tissues of patients with chronic pancreatitis. NEP/CD10 expression in pancreatic tumor lesions and cell lines was not associated with tumor grading and staging. Treatment of PATU-8988T cells with the histone deacetylase inhibitors sodium butyrate and valproic acid induced an increase of NEP/CD10 expression. This was accompanied by a reduced cell proliferation rate of PATU-8988T cells, which was increased by the addition of the enzyme activity inhibitors phosphoramidon and thiorphan. Thus, NEP/CD10 is differentially expressed in pancreatic tumors and might be involved in the proliferative activity of pancreatic cancer cells. However, further studies are needed to provide more detailed information of the role of NEP/CD10 under physiological and pathophysiological conditions of the pancreas. ' 2007 Wiley-Liss, Inc.Key words: neutral endopeptidase (NEP/CD10); pancreatic carcinoma; histone deacetylase inhibitors Cancer of the exocrine pancreas accounts for 2-3% of all cancers, but is the fourth most frequent cause of cancer deaths. 1 Pancreatic cancer is more common among males than females with the peak incidence occurring at age 60. The 5 years survival rate of patients with pancreatic cancer is less than 5%. Although the etiology of this disease remains unclear, cigarette smoking and alcohol abuse have been related with an increased incidence of pancreatic cancer.Neutral endopeptidase (NEP/CD10, EC 3.4.24.11) is a cell-surface metallopeptidase that is normally expressed by numerous tissues including prostate, kidney, breast, intestine, endometrium, adrenal glands and lung. This enzyme disintegrates peptide bonds on the amino terminus of hydrophobic amino acids and inactivates a variety of physiologically active peptides like atrial natriuretic factor, substance P, bradykinin, oxytocin, Leu-and Met-enkephalins, neurotensin, bombesin, calcitonin gene-related peptide, endothelin-1 and bombesin-like peptides. [2][3][4][5][6][7][8][9] NEP/CD10 reduces the local concentration of peptides available for receptor binding and signal transduction. The biological function of NEP/CD10 appears to be organ specific. In...

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Section: Discussionsupporting

confidence: 88%

Expression of neutral endopeptidase (NEP/CD10) on pancreatic tumor cell lines, pancreatitis and pancreatic tumor tissues

Erhuma¹,

Köbel²,

Mustafa³

et al. 2007

Intl Journal of Cancer

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“…Among these, butyrate has previously shown a relevant in vitro antitumoral effect in PC cells, preventing tumor invasion via β4 integrin downregulation [ 119 ], as well as inhibiting proliferation and possibly conditioning cell differentiation and antigen expression [ 120 ]; however, no confirmation studies or recent data regarding the possibility of clinical applications are available.…”

Section: Modulation Of the Gut Microbiotamentioning

confidence: 99%

The Role of Microbiota in Pancreatic Cancer

Papa

Schepis

Coppola

et al. 2023

Cancers

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Pancreatic cancer (PC) has an unfavorable prognosis with few effective therapeutic options. This has led researchers to investigate the possible links between microbiota and PC. A disrupted gut microbiome can lead to chronic inflammation, which is involved in the pathogenesis of PC. In addition, some bacterial strains can produce carcinogens that promote the growth of cancer cells. Research has also focused on pancreatic and oral microbiota. Changes in these microbiota can contribute to the development and progression of PC. Furthermore, patients with periodontal disease have an increased risk of developing PC. The potential use of microbiota as a prognostic marker or to predict patients’ responses to chemotherapy or immunotherapy is also being explored. Overall, the role of microbiota—including the gut, pancreatic, and oral microbiota—in PC is an active research area. Understanding these associations could lead to new diagnostic and therapeutic targets for this deadly disease.

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“…Sodium butyrate inhibits the invasion and metastasis of PC cells by downregulating the expression of integrin β4 ( Farrow et al., 2003 ). It also promotes the differentiation of PC cells and induces the expression of some tumor-related antigens ( Corra et al., 1993 ). These results suggest that butyric acid has a significant inhibitory effect on the occurrence, development and invasion of PC.…”

Section: The Role Of the Gut Microbiota In Prevention And Treatment O...mentioning

confidence: 99%

Potential Roles of the Gut Microbiota in Pancreatic Carcinogenesis and Therapeutics

Yang

Zhang

Zhu

2022

Front. Cell. Infect. Microbiol.

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The intestinal microenvironment is composed of normal gut microbiota and the environment in which it lives. The largest microecosystem in the human body is the gut microbiota, which is closely related to various diseases of the human body. Pancreatic cancer (PC) is a common malignancy of the digestive system worldwide, and it has a 5-year survival rate of only 5%. Early diagnosis of pancreatic cancer is difficult, so most patients have missed their best opportunity for surgery at the time of diagnosis. However, the etiology is not entirely clear, but there are certain associations between PC and diet, lifestyle, obesity, diabetes and chronic pancreatitis. Many studies have shown that the translocation of the gut microbiota, microbiota dysbiosis, imbalance of the oral microbiota, the interference of normal metabolism function and toxic metabolite products are closely associated with the incidence of PC and influence its prognosis. Therefore, understanding the correlation between the gut microbiota and PC could aid the diagnosis and treatment of PC. Here, we review the correlation between the gut microbiota and PC and the research progresses for the gut microbiota in the diagnosis and treatment of PC.

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Modification of antigen expression in human and hamster pancreatic cancer cell lines induced by sodium butyrate

Cited by 6 publications

References 28 publications

Expression of neutral endopeptidase (NEP/CD10) on pancreatic tumor cell lines, pancreatitis and pancreatic tumor tissues

Expression of neutral endopeptidase (NEP/CD10) on pancreatic tumor cell lines, pancreatitis and pancreatic tumor tissues

The Role of Microbiota in Pancreatic Cancer

Potential Roles of the Gut Microbiota in Pancreatic Carcinogenesis and Therapeutics

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