Modification of clastogenicity of three known clastogens by garlic extract in mice in vivo

Abstract

The anticlastogenic activity of crude extract of garlic (Allium sativum L.) was studied in bone marrow cells of mice. Male laboratory-bred Swiss albino mice were given one of three concentrations of the freshly prepared extract (100 mg, 50 mg, and 25 mg/kg body weight) as a dietary supplement by gavage for 6 consecutive days. On the seventh day the mice were administered a single acute dose of two known clastogens, mitomycin C(1.5 mg/kg) and cyclophosphamide (25 mg/kg) or sodium arsenite (2.5 mg/kg), simultaneously with garlic extract. After 24 hr, chromosome preparations were made from the bone marrow cells. The endpoint studied were chromosomal aberrations and damaged cells. Garlic extract alone induced a low level of chromosomal damage. The clastogenicity of all three mutagens were reduced significantly in the animals which had been given garlic extract as dietary supplement. The extent of reduction was different for the three clastogens and may be attributed to the interaction with the different components of the extract.

A review on exposure and effects of arsenic in passerine birds

“…Rat and rabbit Mukherjee et al (2003Mukherjee et al ( , 2004 Cancer Liver, adrenal, ovarian and lung tumors Mouse Cui et al (2006); Waalkes et al (2003) Genotoxicity Somatic mutations and mitotic recombination, chromosomal aberrations (bone marrow cells), Drosophila melanogaster, rat and Das et al (1993); Datta et al (1986);McDorman et al (2002); Poddar et al (2000); Ramos-Morales and Rodríguez-Arnaiz (1995) (continued on next page) (Geens et al, 2010;Hofer et al, 2010). Moreover, breeding parameters such as clutch size, length of the incubation period or hatching and fledging success are evaluated to study the effects of As and metals in breeding performance.…”

Protection of N‐methyl‐N′‐nitro‐N‐nitrosoguanidine‐induced in vivo clastogenicity by aqueous garlic extract

“…19 Free radicals, primarily hydroxyl radicals, have been suggested to play a key role in the mutagenic effects of MNNG. 13,22,23 Allyl sulfides, important constituents of garlic, were found to exhibit antigenotoxic effects against a variety of carcinogens. 20,21 Pretreatment with garlic significantly reduced the clastogenic potency of MNNG.…”

Allium sativum

“…The clastogenicity of three mutagens, clastogens, mitomycin C(1.5 mg/kg) and cyclophosphamide (25 mg/kg) or sodium arsenite (2.5 mg/ kg), were reduced signifi cantly in mice which had been given garlic extract as a dietary supplement (Das et al 1993 ). Addition of 2 % garlic powder to diets containing aminopyrine and sodium nitrite (each at 600 mg/kg) reduced the occurrence of both 7-N-methyldeoxyguanosine (7-N-mG) and 6-O -methyldeoxyguanosine (6-O -mG) adducts to rat liver DNA by approximately 55 % and over 80 % when 4 % garlic was provided .…”