Modification of DNA Checkpoints to Confer Aluminum Tolerance

Eekhout, Thomas; Larsen, Paul B.; Veylder, Lieven De

doi:10.1016/j.tplants.2016.12.003

Cited by 49 publications

(37 citation statements)

References 12 publications

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“…There are a number of symptoms caused by aluminum toxicity in plants ( Figure 3 ), all associated with severe changes in the root system ( Kopittke and Blamey, 2016 ). Al interferes with cell division at the root apex and lateral roots, increases the rigidity of the cell wall by cross-linking of pectins and reduces DNA replication because of increased rigidity of the double helix ( Zhang et al, 2014 ; Eekhout et al, 2017 ). Moreover, Al induces a series of cellular toxic changes concerning cell division and nucleolus, and localization and expression of the nucleolar proteins such as fibrillarin ( Zhang et al, 2014 ).…”

Section: Aluminum Toxicitymentioning

confidence: 99%

Aluminum, a Friend or Foe of Higher Plants in Acid Soils

et al. 2017

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Aluminum (Al) is the most abundant metal in the earth’s crust, but its availability depends on soil pH. Despite this abundance, Al is not considered an essential element and so far no experimental evidence has been put forward for a biological role. In plants and other organisms, Al can have a beneficial or toxic effect, depending on factors such as, metal concentration, the chemical form of Al, growth conditions and plant species. Here we review recent advances in the study of Al in plants at physiological, biochemical and molecular levels, focusing mainly on the beneficial effect of Al in plants (stimulation of root growth, increased nutrient uptake, the increase in enzyme activity, and others). In addition, we discuss the possible mechanisms involved in improving the growth of plants cultivated in soils with acid pH, as well as mechanisms of tolerance to the toxic effect of Al.

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Section: Aluminum Toxicitymentioning

confidence: 99%

Aluminum, a Friend or Foe of Higher Plants in Acid Soils

et al. 2017

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“…This is in contrast to a loss‐of‐function mutant for ATM (ataxia telangiectasia mutated), which has little effect on Al tolerance as measured by suppression of als3‐1 . Mutants ATR, ATRIP, SOG1 and TAN/MEI/ALT2 grow even better during long‐term exposure to Al compared with the wild‐type (WT), leaving it unclear whether and, if so, how Al induces DNA damage (Larsen et al ., ; Rounds and Larsen, ; Nezames et al ., ; Sjogren et al ., ; Eekhout et al ., ; Sjogren and Larsen, ).…”

Section: Introductionmentioning

confidence: 98%

A multi‐level response to DNA damage induced by aluminium

et al. 2019

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SummaryAluminium (Al) ions are one of the primary growth‐limiting factors for plants on acid soils, globally restricting agriculture. Despite its impact, little is known about Al action in planta. Earlier work has indicated that, among other effects, Al induces DNA damage. However, the loss of major DNA damage response regulators, such SOG1, partially suppressed the growth reduction in plants seen on Al‐containing media. This raised the question whether Al actually causes DNA damage and, if so, how. Here, we provide cytological and genetic data corroborating that exposure to Al leads to DNA double‐strand breaks. We find that the Al‐induced damage specifically involves homology‐dependent (HR) recombination repair. Using an Al toxicity assay that delivers higher Al concentrations than used in previous tests, we find that sog1 mutants become highly sensitive to Al. This indicates a multi‐level response to Al‐induced DNA damage in plants.

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“…Three rice genotypes (ARR 09 (AR); IR1552 (IR), and theruvii (TH)) were selected for generating NGS data for seedling stage Al toxicity tolerance. Seeds (30)(31)(32)(33)(34)(35)(36)(37)(38)(39)(40) of the selected genotypes were germinated on petri plates containing moist filter paper and kept in the green house. The 5-day old seedlings of each genotype were transferred to full strength Magnavaca's solution containing 0.54 mM AlCl 3 to create Al toxic hydroponic condition 51 .…”

Section: Methodsmentioning

confidence: 99%

Root transcriptome reveals efficient cell signaling and energy conservation key to aluminum toxicity tolerance in acidic soil adapted rice genotype

Tyagi

Yumnam

Sen

et al. 2020

Sci Rep

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Aluminium (Al) toxicity is the single most important contributing factor constraining crop productivity in acidic soils. Hydroponics based screening of three rice genotypes, a tolerant (ARR09, AR), a susceptible (IR 1552, IR) and an acid soil adapted landrace (Theruvii, TH) revealed that AR accumulates less Al and shows minimum decrease in shoot and root biomass under Al toxicity conditions when compared with IR. Transcriptome data generated on roots (grown in presence or absence of Al) led to identification of ~1500 transcripts per genotype with percentage annotation ranging from 21.94% (AR) to 29.94% (TH). A total of 511, 804 and 912 DEGs were identified in genotypes AR, IR and TH, respectively. IR showed upregulation of transcripts involved in exergonic processes. AR appears to conserve energy by downregulating key genes of glycolysis pathway and maintaining transcript levels of key exergonic step enzymes under Al stress. The tolerance in AR appears to be as a result of novel mechanism as none of the reported Al toxicity genes or QTLs overlap with significant DEGs. Components of signal transduction and regulatory machinery like transcripts encoding zinc finger protein, calcieurin binding protein and cell wall associated transcripts are among the highly upregulated DEGs in AR, suggesting increased and better signal transduction in response to Al stress in tolerant rice. Sequencing of NRAT1 and glycine-rich protein A3 revealed distinct haplotype for indica type AR. The newly identified components of Al tolerance will help in designing molecular breeding tools to enhance rice productivity in acidic soils.www.nature.com/scientificreports www.nature.com/scientificreports/ downregulated, respectively. Similarly, 804 DEGs were identified in IR, out of which 219 were upregulated and 368 were downregulated. In the case of TH, out of a total of 912 DEGs, only 37 transcripts showed significant upregulation in presence of Al 3+ (Fig. 2B). Scientific RepoRtS |(2020) 10:4580 | https://doi. Validation of selected up-regulated DEGs by Q-PCR.A set of 15 genes were randomly selected and validated by qRT-PCR analysis in three biological replicates for confirming the results obtained from DEG analysis. The analysis revealed similar expression pattern for all the selected genes in qPCR analysis as observed from RNA-seq data. The statistical analysis also showed significant association (r 2 = 0.78) between the results of qPCR and RNA-seq data analyses (Fig. 2C).Scientific RepoRtS | (2020) 10:4580 | https://doi.National Center for Biotechnology Information (NCBI) was used for BLASTx 56 (E value ≤0.00001; annotation cutoff = 55; GO weight = 5). The transcriptome for the three genotypes was analyzed for a) DEGs and b) transcripts which were specific to the Al concentration (treatment (0.54 mM Al 3+ )).

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Modification of DNA Checkpoints to Confer Aluminum Tolerance

Cited by 49 publications

References 12 publications

Aluminum, a Friend or Foe of Higher Plants in Acid Soils

Aluminum, a Friend or Foe of Higher Plants in Acid Soils

A multi‐level response to DNA damage induced by aluminium

Root transcriptome reveals efficient cell signaling and energy conservation key to aluminum toxicity tolerance in acidic soil adapted rice genotype

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