Modification of gene expression induced in human osteogenic and osteosarcoma cells by culture on a biphasic calcium phosphate bone substitute

Three-dimensional culture and differentiation of human osteogenic cells in an injectable hydroxypropylmethylcellulose hydrogel

“…The CAL72 human osteosarcoma cell line was a generous gift of Dr. J Gioanni (Centre Antoine Lacassagne, Nice, France) and has been described previously [23][24][25]. The human osteosarcoma cell lines MG-63 and SaOS-2 were obtained from the American Type Culture Collection (Manassas, VA).…”

Cultivation and identification of rat bone marrow-derived mesenchymal stem cells

“…However, a lack of sufficient materials precludes the use of autologous bone while the use of allogenic bone for transplantation carries a potential risk of immune responses. Progress in materials science and biology has resulted in the possibility of bone tissue engineering with the aim of producing a bony equivalent in vitro by combining bone forming cells and a synthetic three-dimensional scaffold (2)(3)(4).…”

Bone Formation Deregulations Are the Oncogenesis Keys in Osteosarcomas

“…Looking closely to these normal features, OS cells are really comparable to undifferentiated osteogenic precursors with a high proliferative capacity, a resistance to apoptosis and a differential expression of osteogenic markers, such as CTGF, Runx2, ALP, Osterix, Osteopontin and Osteocalcin. In fact, the late osteogenic markers, Osteocalcin and Osteopontin, and the early markers of osteogenesis, like ALP, are less expressed than in normal osteoblasts, whereas growth factors are up-regulated or down-regulated almost as in normal osteogenic cells (Luu et al, 2007;Rochet et al, 2003). Usually, malignant osteoblastic cells fail to undergo terminal osteoblastic differentiation.…”