Modification of Modeling Method of Toxic Dystrophy of Liver in Rats

Gryshchenko, V. A.; Danchenko, Olena; Musiychuk, V. V.

doi:10.1007/978-3-030-14918-5_67

Cited by 17 publications

(20 citation statements)

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“…This explains the low level of lactate, citrate, pyruvate in healthy newborn calves. The pathological state of the digestive canal leads to a violation of the electrolyte balance of the organism [27,46,47] and affects the function of the epithelium [48][49][50][51], and according to our data changes intracellular homeostasis. The high level of citrate (18.8 times higher) in the epithelium of the small intestine of patients with enteropathology of newborn calves, in comparison with healthy calves, indicates the inhibition of oxidative processes in the mitochondria, which may be due either to insufficiency of the oxygen supply of the epithelium [52], or to inhibition by another mechanism.…”

Section: Preparation Of Intestinal Epithelial Cells By Various Methodologicalmentioning

confidence: 99%

Trends in the development of Medicine, Biology and Pharmacy

2021

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Section: Preparation Of Intestinal Epithelial Cells By Various Methodologicalmentioning

confidence: 99%

Trends in the development of Medicine, Biology and Pharmacy

2021

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“…At the beginning of the experiment, three groups of rats were formed (control and two experimental), 20 animals each. To reproduce the subacute form of fatty hepatosis according to our modified method, the rats of the first and second research groups (Gryshchenko et al, 2019) were for 7 days intragastrically administered a suspension of tetracycline hydrochloride in a 4% starch gel solution once a day at a dose of 250 mg/kg (0.25 LD 25 ) using a probe. The animals in the control group were given a similar volume of double-distilled water.…”

Section: Methodsmentioning

confidence: 99%

Fatty acids of lipids of blood serum and liver of rats with tetracyclin-induced hepatosis and at correction

Gryshchenko

Sуsoliatin

Мидык

2019

Regul. Mech. Biosyst.

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Irrational pharmacotherapy with tetracyclines can cause mitochondrial cytopathies of liver cells and the development of toxic dystrophy. The issue of violation of the qualitative and quantitative composition of fatty acids and their functional role in the development of liver dystrophy has not been studied enough. Therefore, the aim of the work was to determine the features of changes in the fatty acid composition of blood serum and liver lipids in rats with tetracycline-induced hepatosis and the corrective efficacy of the phospholipid-acceptable dietary supplement “FLP-MD”. It was experimentally established that, in the composition of blood serum lipids and liver tissue, key changes in modeling tetracycline-induced hepatosis in rats (250 mg/kg for 7 days) are primarily tested for polyene fatty acids (PUFAs). In blood serum this is manifested by a decrease in the level of docosadiienoic (22:2ω6) and docosahexaenoic acid (22:6ω3), as well as the total PUFA content due to representatives of the ω3 family, respectively, by 1.3 times. At the same time, a decrease in the content of eicosapentenoic (20:5ω3), docosapentaenoic (22:5ω3), docosahexaenoic (22:6ω3) acids and the saturated/unsaturated fatty acid (SFA/UFA) and ω3/ω6 PUFA ratios, respectively, are noted in the lipid fraction of the liver, respectively by 1.3 times. In contrast to these animals, the use of reparative action of a phospholipid-acceptable dietary supplement “FLP-MD” (13.5 mg/kg) in sick rats provides a significant increase in the level of these fatty acids both in blood serum and in the liver and the restoration of their profile for other representatives. Thus, the components of the “FLP-MD” dietary supplement show a corrective effect on the fatty acid composition of the lipid component of blood serum and liver tissue of animals with tetracycline-induced hepatosis. Indicators have been experimentally established for the diagnosis of toxic liver damage and preclinical trials of the hepatoprotective efficacy of new drugs, which is a promising area of research in veterinary hepatopathology.

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“…A significant and diverse arsenal of modern therapeutic and prophylactic agents is not always effective in treating patients with enteropathology of newborn animals, which is explained by the need for timely diagnosis of the development of acute digestive disorders and monitoring the functional condition of this system. At the same time, the restoration of metabolic processes and the structural and functional condition of epithelial cells of the intestinal mucosa, hepatocytes does not end three weeks after the clinical recovery of newborn calves [8][9][10][11].…”

Section: Section 1 Biology 11 Correcting Effectiveness Of Milk Phospholipides By Calves Enteropatнologymentioning

confidence: 99%

“…Decreases complex carbohydrates which may affect bowel function. (8) The Maker's Diet -Focuses on four components of total health-physical, mental, spiritual, and emotional. Consists of a phased approach.…”

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confidence: 99%

Traditional medicine and pharmacology. Achievements, innovations, and alternatives

Vladlenov¹

2021

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Modification of Modeling Method of Toxic Dystrophy of Liver in Rats

Cited by 17 publications

References 17 publications

Trends in the development of Medicine, Biology and Pharmacy

Trends in the development of Medicine, Biology and Pharmacy

Fatty acids of lipids of blood serum and liver of rats with tetracyclin-induced hepatosis and at correction

Traditional medicine and pharmacology. Achievements, innovations, and alternatives

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