Modification of pectin distribution in sunflower (Helianthus annuus L.) roots in response to lead exposure

Chudzik, Barbara; Szczuka, Ewa; Leszczuk, Agata; Strubińska, Joanna

doi:10.1016/j.envexpbot.2018.07.005

Cited by 21 publications

(8 citation statements)

References 41 publications

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“…These cell wall components provide binding and adsorption sites for heavy metals before crossing the cell membrane leading to less plant heavy metal accumulation in the cytoplasm [18,29,[31][32][33][34][35][36][37][38]. Previous experiments indicate that the content of total sugars and uronic acid in the PE and HE of the cell wall increase significantly under Al and Cu stress [24,39], and an experiment found that high lead ion stress impacts the homogalacturonan content of the uronic acid in Helianthus annuus L. root zones [40]. Xylogalacturonan from pectic uronic acid was proven to exert a vital role in increasing the Cd tolerance of Medicago sativa [38].…”

Section: Introductionmentioning

confidence: 99%

Effects of the Dark Septate Endophyte (DSE) Exophiala pisciphila on the Growth of Root Cell Wall Polysaccharides and the Cadmium Content of Zea mays L. under Cadmium Stress

Xiao

Dai

Zhang

et al. 2021

JoF

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This paper aims to investigate the mechanism by which dark septate endophytes (DSEs) enhance cadmium (Cd) tolerance in there host plants. Maize (Zea mays L.) was inoculated with a DSE, Exophiala pisciphila, under Cd stress at different concentrations (0, 5, 10, and 20 mg·kg−1). The results show that, under 20 mg/kg Cd stress, DSE significantly increased maize biomass and plant height, indicating that DSE colonization can be utilized to increase the Cd tolerance of host plants. More Cd was retained in DSE-inoculated roots, especially that fixed in the root cell wall (RCW). The capability of DSE to induce a higher Cd holding capacity in the RCW is caused by modulation of the total sugar and uronic acid of DSE-colonized RCW, mainly the pectin and hemicellulose fractions. The fourier-transform spectroscopy analysis results show that carboxyl, hydroxyl, and acidic groups are involved in Cd retention in the DSE-inoculated RCW. The promotion of the growth of maize and improvement in its tolerance to Cd due to DSEs are related to restriction of the translocation of Cd from roots to shoots; resistance of Cd uptake Cd inside cells; and the increase in RCW-integrated Cd through modulating RCW polysaccharide components.

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Section: Introductionmentioning

confidence: 99%

Effects of the Dark Septate Endophyte (DSE) Exophiala pisciphila on the Growth of Root Cell Wall Polysaccharides and the Cadmium Content of Zea mays L. under Cadmium Stress

Xiao

Dai

Zhang

et al. 2021

JoF

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“…More sucrose and maltose may improve strain’s ability to dissolve phosphorus in the environment ( Mukhtar et al, 2017 ). Pectin is a component in the plant cell wall ( Chudzik et al, 2018 ). It also can be found in secretions of rhizosphere and utilized by rhizobacteria ( Li et al, 2012 ).…”

Section: Discussionmentioning

confidence: 99%

Multiple Metabolic Phenotypes as Screening Criteria Are Correlated With the Plant Growth-Promoting Ability of Rhizobacterial Isolates

Shi

Zhang

et al. 2022

Front. Microbiol.

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Efficient screening method is the prerequisite for getting plant growth-promoting (PGP) rhizobacteria (PGPR) which may play an important role in sustainable agriculture from the natural environment. Many current traditional preliminary screening criteria based on knowledge of PGP mechanisms do not always work well due to complex plant–microbe interactions and may lead to the low screening efficiency. More new screening criteria should be evaluated to establish a more effective screening system. However, the studies focused on this issue were not enough, and few new screening criteria had been proposed. The aim of this study was to analyze the correlation between the metabolic phenotypes of rhizobacterial isolates and their PGP ability. The feasibility of using these phenotypes as preliminary screening criteria for PGPR was also evaluated. Twenty-one rhizobacterial isolates were screened for their PGP ability, traditional PGP traits, and multiple metabolic phenotypes that are not directly related to PGP mechanisms, but are possibly related to rhizosphere colonization. Correlations between the PGP traits or metabolic phenotypes and increases in plant agronomic parameters were analyzed to find the indicators that are most closely related to PGP ability. The utilization of 11 nutrient substrates commonly found in root exudates, such as D-salicin, β-methyl-D-glucoside, and D-cellobiose, was significantly positively correlated with the PGP ability of the rhizobacterial isolates. The utilization of one amino acid and two organic acids, namely L-aspartic acid, α-keto-glutaric acid, and formic acid, was negatively correlated with PGP ability. There were no significant correlations between four PGP traits tested in this study and the PGP ability. The ability of rhizobacterial isolates to metabolize nutrient substrates that are identical or similar to root exudate components may act as better criteria than PGP traits for the primary screening of PGPR, because rhizosphere colonization is a prerequisite for PGPR to affect plants.

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“…For the histiocyte labeling of pectin, cellulose, and lignin, the paraffin sections of juice sacs were prepared according to the protocol of Li et al (2017) with slight modifications. The pectin was labeled by immunofluorescence staining with JIM5 and JIM7 (Plant Probes, Leeds, United Kingdom) for the labeling of low-methylesterified pectin and high-methylesterified pectin, respectively, according to the method of Chudzik et al (2018) with slight modification. The cellulose was stained with Calcofluor White (Coolaber, Beijing, China) according to the manufacturers’ instructions and observed in the excitation wavelength of 405 nm.…”

Section: Methodsmentioning

confidence: 99%

Integrated Physiochemical, Hormonal, and Transcriptomic Analysis Revealed the Underlying Mechanisms for Granulation in Huyou (Citrus changshanensis) Fruit

et al. 2022

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Juice sac granulation is a common internal physiological disorder of citrus fruit. In the present study, we compared the physiochemical characteristics and transcriptome profiles of juice sacs in different granulation levels from Huyou fruit (Citrus changshanensis). The accumulation of cell wall components, including the water-soluble pectin, protopectin, cellulose, and lignin, were significantly correlated with the granulation process, resulting in the firmness increase of the juice sac. The in situ labeling of the cell wall components indicated the early accumulation of cellulose and high-methylesterified pectin in the outer layer cells, as well as the late accumulation of lignin in the inner layer cells of the juice sac. Several phytohormones, including auxins, abscisic acids, cytokinins, jasmonic acid, salicylic acid, and/or their metabolites, were positively correlated to the granulation level, indicating an active and complex phytohormones metabolism in the granulation process. Combining the trend analysis by the Mfuzz method and the module-trait correlation analysis by the Weighted Gene Co-expression Network Analysis method, a total of 2940 differentially expressed genes (DEGs) were found to be positively correlated with the granulation level. Gene Ontology (GO) enrichment indicated that the selected DEGs were mainly involved in the cell wall organization and biogenesis, cell wall macromolecule metabolic process, carbohydrate metabolic process, and polysaccharide metabolic process. Among these selected genes, those encoding β-1,4-xylosyltransferase IRX9, cellulose synthase, xyloglucan: xyloglucosyl transferase, xyloglucan galactosyltransferase MUR3, α-1,4-galacturonosyltransferase, expansin, polygalacturonase, pectinesterase, β-glucosidase, β-galactosidase, endo-1,3(4)-β-glucanase, endoglucanase and pectate lyase that required for the biosynthesis or structural modification of cell wall were identified. In addition, NAC, MYB, bHLH, and MADS were the top abundant transcription factors (TFs) families positively correlated with the granulation level, while the LOB was the top abundant TFs family negatively correlated with the granulation level.

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Modification of pectin distribution in sunflower (Helianthus annuus L.) roots in response to lead exposure

Cited by 21 publications

References 41 publications

Effects of the Dark Septate Endophyte (DSE) Exophiala pisciphila on the Growth of Root Cell Wall Polysaccharides and the Cadmium Content of Zea mays L. under Cadmium Stress

Effects of the Dark Septate Endophyte (DSE) Exophiala pisciphila on the Growth of Root Cell Wall Polysaccharides and the Cadmium Content of Zea mays L. under Cadmium Stress

Multiple Metabolic Phenotypes as Screening Criteria Are Correlated With the Plant Growth-Promoting Ability of Rhizobacterial Isolates

Integrated Physiochemical, Hormonal, and Transcriptomic Analysis Revealed the Underlying Mechanisms for Granulation in Huyou (Citrus changshanensis) Fruit

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