Modification of Perennial Fruit Trees

Deng, Xiuxin; Duan, Yanxin

doi:10.1007/3-540-32199-3_3

Cited by 10 publications

(3 citation statements)

References 82 publications

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“…The improvement of fruit quality by traditional breeding techniques has been constrained by complex biology of citrus, especially the long juvenile period. Genetic transformation provides the means for adding a single agronomic trait in perennial plant cultivars without altering their phenotype (Deng and Duan 2006). Until now, various genes have been introduced into citrus species and its relatives to improve agronomical traits, including enhancing abiotic stress tolerance (Cervera et al 2000;Fagoaga et al 2007) and disease resistance (Ananthakrishnan et al 2007;GonzalezRamos et al 2005;Omar et al 2007;Zanek et al 2008), shortening the juvenile phase (Duan et al 2007a;Endo et al 2005;Peña et al 2001), rootstock (Gentile et al 2004), and fruit improvement (Costa et al 2002;Guo et al 2005;Koltunow et al 2000;Li et al 2002;Wong et al 2001).…”

Section: Introductionmentioning

confidence: 97%

Highly efficient transformation of the GFP and MAC12.2 genes into precocious trifoliate orange (Poncirus trifoliata [L.] Raf), a potential model genotype for functional genomics studies in Citrus

Tan

et al. 2009

Tree Genetics & Genomes

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Precocious trifoliate orange (Poncirus trifoliata [L.] Raf), an extremely early flowering mutant of P. trifoliata, is an attractive model for functional genomics research in Citrus. A procedure for efficient regeneration and transformation of this genotype was developed by using green fluorescent protein (GFP) gene as visual marker and etiolated stem segments as explants. In vivo monitoring of GFP expression permitted a rapid and easy discrimination of transgenic shoots and escapes. Transformation efficiency was 20.7% and the transformants were identified by polymerase chain reaction (PCR) and Southern blot analysis. Moreover, the transgenic lines expressed variable amounts of the GFP gene as revealed by real-time PCR analysis. Fifteen transgenic plants flowered 18 months after transfer to the greenhouse and six of them set fruits. GFP expression was also observed in the transgenic flowers and fruits. To test the utility of this system for functional genomics studies, an Arabidopsis thaliana MAC12.2 gene with the potential to produce seedless fruits was introduced into this genotype, and the traits of the transgenic fruits were characterized. The successful transformation of this perennial woody genotype with extremely short juvenility will allow us to test the function of cloned genes in citrus, the improvement of which is hindered by a long juvenility period.

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Section: Introductionmentioning

confidence: 97%

Highly efficient transformation of the GFP and MAC12.2 genes into precocious trifoliate orange (Poncirus trifoliata [L.] Raf), a potential model genotype for functional genomics studies in Citrus

Tan

et al. 2009

Tree Genetics & Genomes

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“…Despite the need for new and improved cultivars the rate of development is slow due to long juvenile period, high heterozygosity and sexual incompatibility factors (Deng and Duan 2006). Gene transfer technologies such as Agrobacterium-mediated transformation Grosser 2009, 2010;Pasquali et al 2009;Ballester et al 2010;Duan et al 2010;Dutt et al 2011), particle gun bombardment and protoplast fusion (Wang et al 2010;Grosser and Gmitter 2011) offer an alternative approach for the transfer of gene traits into important germplasms.…”

Section: Introductionmentioning

confidence: 99%

Influence of surfactants on growth and regeneration from mature internodal stem segments of sweet orange (Citrus sinensis) cv. Hamlin

Curtis

Mirkov

2011

Plant Cell Tiss Organ Cult

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The development of a reliable shoot regeneration system for mature tissue of citrus is of major importance to accelerate the evaluation of commercial traits. Three non-ionic surfactants were evaluated independently in terms of their affects on the growth and regeneration of mature internodal stem segments of sweet orange cv. Hamlin in culture. Growth and shoot development of explants were influenced by type of surfactant added to the regeneration medium DBA3, its concentration and order of flush growth used for explant preparation.

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“…The juvenile stage of Citrus plants propagated by seeds can last from 5 to 22 years, depending on the species and genotype [ 2 ]. Consequently, genetic transformation has been considered an important breeding alternative for Citrus as a mean of introducing desirable traits in elite cultivars without affecting a highly favorable genotypic combination, by avoiding recombination during meiosis [ 3 ].…”

Section: Introductionmentioning

confidence: 99%

Early-flowering sweet orange mutant ‘x11’ as a model for functional genomic studies of Citrus

Pinheiro

Figueira

Latado³

2014

BMC Res Notes

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BackgroundThere had been many reports on genetic transformation of Citrus for functional genomic studies but few included genes associated with flower or fruit traits. A major reason for this might derive from the extensive juvenile stage of Citrus plants when regenerated from juvenile explants (epicotyls, cotyledon or calli), which delays the observation of the resulting phenotype. Alternatives include the use of explants from adult tissues, which sometimes may be recalcitrant to regeneration or transformation, or of early-flowering genotypes. However, there is no report about the use of early-flowering sweet orange mutants for functional genomic studies.ResultsHere, we propose a sweet orange spontaneous early-flowering mutant, named ‘x11’, as a platform for Citrus functional genomic studies, particularly for genes associated with flower or fruit traits. We report a procedure for efficient regeneration and transformation using epicotyl segment explants of ‘x11’ and Agrobacterium tumefaciens as a proof-of-concept. The average transformation efficiency was 18.6%, but reached 29.6% in the best protocol tested. Among 270 positive shoots, five were in vitro micrografted and acclimatized, followed by evaluation of transgene expression by quantitative amplification of reversed transcripts (RT-qPCR) and determination of the number of copies inserted. Four of these plants, containing from one to four copies of the transgene, exhibited the first flowers within three months after ex vitro establishment, and the other, two months later, regardless of the period of the year. Flowers of transgenic plants displayed fertile pollen and gynoecium, with self-pollination inducing fruit development with seeds. Histochemical staining for β-glucuronidase activity using stem segments, flowers and fruits from 5 to 7 month-old acclimatized transgenic plants confirmed the constitutive transgene expression in these organs.ConclusionThe ‘x11’ sweet orange is suitable for functional genomics studies with a satisfactory transformation rate, and it can be considered a good model for functional genomic studies in commercial sweet oranges, for traits related to flower and fruit.Electronic supplementary materialThe online version of this article (doi:10.1186/1756-0500-7-511) contains supplementary material, which is available to authorized users.

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Modification of Perennial Fruit Trees

Cited by 10 publications

References 82 publications

Highly efficient transformation of the GFP and MAC12.2 genes into precocious trifoliate orange (Poncirus trifoliata [L.] Raf), a potential model genotype for functional genomics studies in Citrus

Highly efficient transformation of the GFP and MAC12.2 genes into precocious trifoliate orange (Poncirus trifoliata [L.] Raf), a potential model genotype for functional genomics studies in Citrus

Influence of surfactants on growth and regeneration from mature internodal stem segments of sweet orange (Citrus sinensis) cv. Hamlin

Early-flowering sweet orange mutant ‘x11’ as a model for functional genomic studies of Citrus

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