Modification of plasma membrane H+-ATPase in Masson pine (<i>Pinus massoniana</i> Lamb.) seedling roots adapting to acid deposition

Zhou, Shaoxia; Wang, Ping; Ding, Yi; Xie, Linbei; Li, Ao

doi:10.1093/treephys/tpac015

Cited by 5 publications

(4 citation statements)

References 85 publications

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“…In addition, H + ‐ATPase is capable of responding to salt and high pH, temperature, drought and heavy metal stresses (Jakubowska et al, 2015; Li et al, 2022; Pei et al, 2022; Sadura et al, 2020; Yang et al, 2006). In terms of regulation of H + ‐ATPase, most research focus on its transcriptional regulation and post translational phosphorylation modification (Lin et al, 2021; Minami et al, 2019; Zhang et al, 2021; Zhou et al, 2022). The transcription factors that have been reported to regulate the activity of H + ‐ATPase include MYB, ERF, WRKY and MBW complexes (Fan et al, 2022; Xu et al, 2014; Yao et al, 2020; Yu et al, 2017).…”

Section: Discussionmentioning

confidence: 99%

Mitogen‐activated protein kinase MxMPK3‐2 mediated phosphorylation of MxZR3.1 participates in regulating iron homoeostasis in apple rootstocks

Li,

Zhai,

et al. 2024

Plant Cell & Environment

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The micronutrient iron plays a crucial role in the growth and development of plants, necessitating meticulous regulation for its absorption by plants. Prior research has demonstrated that the transcription factor MxZR3.1 restricts iron absorption in apple rootstocks; however, the precise mechanism by which MxZR3.1 contributes to the regulation of iron homoeostasis in apple rootstocks remains unexplored. Here, MxMPK3‐2, a protein kinase, was discovered to interact with MxZR3.1. Y2H, bimolecular fluorescence complementation and pull down experiments were used to confirm the interaction. Phosphorylation and cell semi‐degradation tests have shown that MxZR3.1 can be used as a substrate of MxMPK3‐2, which leads to the MxZR3.1 protein being more stable. In addition, through tobacco transient transformation (LUC and GUS) experiments, it was confirmed that MxZR3.1 significantly inhibited the activity of the MxHA2 promoter, while MxMPK3‐2 mediated phosphorylation at the Ser94 site of MxZR3.1 further inhibited the activity of the MxHA2 promoter. It is tightly controlled to absorb iron during normal growth and development of apple rootstocks due to the regulatory effect of the MxMPK3‐2‐MxZR3.1 module on MxHA2 transcription level. Consequently, this research has revealed the molecular basis of how the MxMPK3‐2‐MxZR3.1 module in apple rootstocks controls iron homoeostasis by regulating the MxHA2 promoter's activity.

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Section: Discussionmentioning

confidence: 99%

Mitogen‐activated protein kinase MxMPK3‐2 mediated phosphorylation of MxZR3.1 participates in regulating iron homoeostasis in apple rootstocks

Li,

Zhai,

et al. 2024

Plant Cell & Environment

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“…Therefore, many studies have speculated that RCI2-like genes may regulate other ion transporters or membrane proteins to function [ 19 , 33 ]. Several studies have shown that H + -ATPase can maintain the intracellular pH level under alkaline stress [ 32 , 34 , 35 ]. PM H + -ATPase is a key regulator of NaCl tolerance, as it provides a proton-driving force for Na + /H + exchange [ 36 ].…”

Section: Discussionmentioning

confidence: 99%

Overexpression of MsRCI2D and MsRCI2E Enhances Salt Tolerance in Alfalfa (Medicago sativa L.) by Stabilizing Antioxidant Activity and Regulating Ion Homeostasis

Zhang

et al. 2022

IJMS

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Rare cold-inducible 2 (RCI2) genes from alfalfa (Medicago sativa L.) are part of a multigene family whose members respond to a variety of abiotic stresses by regulating ion homeostasis and stabilizing membranes. In this study, salt, alkali, and ABA treatments were used to induce MsRCI2D and MsRCI2E expression in alfalfa, but the response time and the expression intensity of the MsRCI2D,-E genes were different under specific treatments. The expression intensity of the MsRCI2D gene was the highest in salt- and alkali-stressed leaves, while the MsRCI2E gene more rapidly responded to salt and ABA treatment. In addition to differences in gene expression, MsRCI2D and MsRCI2E differ in their subcellular localization. Akin to MtRCI2D from Medicago truncatula, MsRCI2D is also localized in the cell membrane, while MsRCI2E is different from MtRCI2E, localized in the cell membrane and the inner membrane. This difference might be related to an extra 20 amino acids in the C-terminal tail of MsRCI2E. We investigated the function of MsRCI2D and MsRCI2E proteins in alfalfa by generating transgenic alfalfa chimeras. Compared with the MsRCI2E-overexpressing chimera, under high-salinity stress (200 mmol·L−1 NaCl), the MsRCI2D-overexpressing chimera exhibited a better phenotype, manifested as a higher chlorophyll content and a lower MDA content. After salt treatment, the enzyme activities of SOD, POD, CAT, and GR in MsRCI2D- and -E-overexpressing roots were significantly higher than those in the control. In addition, after salt stress, the Na+ content in MsRCI2D- and -E-transformed roots was lower than that in the control; K+ was higher than that in the control; and the Na+/K+ ratio was lower than that in the control. Correspondingly, H+-ATPase, SOS1, and NHX1 genes were significantly up-regulated, and the HKT gene was significantly down-regulated after 6 h of salt treatment. MsRCI2D was also found to regulate the expression of the MsRCI2B and MsRCI2E genes, and the MsRCI2E gene could alter the expression of the MsRCI2A, MsRCI2B, and MsRCI2D genes. MsRCI2D- and -E-overexpressing alfalfa was found to have higher salt tolerance, manifested as improved activity of antioxidant enzymes, reduced content of reactive oxygen species, and sustained Na+ and K+ ion balance by regulating the expression of the H+-ATPase, SOS1, NHX1, HKT, and MsRCI2 genes.

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“…Unlike SOS1 and SOS2 genes, under normal conditions, P. indica colonization did not change the transcription level of the PM-H + -ATPase gene, but after salt stress, the PM-H + -ATPase gene was significantly up-regulated compared with that in uncolonized soybean. Several studies have shown that H + -ATPase can maintain the intracellular pH level under alkaline stress [ 51 , 52 , 53 ]. PM-H + -ATPase is a key regulator of NaCl tolerance as it provides a proton-driving force for Na + /H + exchange [ 54 ].…”

Section: Discussionmentioning

confidence: 99%

Symbiotic System Establishment between Piriformospora indica and Glycine max and Its Effects on the Antioxidant Activity and Ion-Transporter-Related Gene Expression in Soybean under Salt Stress

Zhang

Wang²,

Zhang³

et al. 2022

IJMS

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The utilization of symbiosis with beneficial microorganisms has considerable potential for increasing growth and resistance under abiotic stress. The endophytic root fungus Piriformospora indica has been shown to improve plant growth under salt and drought stress in diverse plant species, while there have been few reports of the interaction of P. indica with soybean under salt stress. In this study, the symbiotic system of P. indica and soybean (Glycine max L.) was established, and the effect of P. indica on soybean growth and salt tolerance was investigated. The colonized and non-colonized soybeans were subjected to salt stress (200 mmol/L NaCl), and the impairments in chlorophyll and increasing relative conductivity that can be caused by salt stress were alleviated in the P. indica-colonized plants. The accumulation of malondialdehyde (MDA), hydrogen peroxide (H2O2), and superoxide anion (O2−) were lower than that in non-colonized plants under salt treatment, whereas the activities of antioxidant enzymes were significantly increased by P. indica colonization, including superoxide dismutase (SOD), peroxidase (POD), catalase (CAT), and glutathione reductase (GR). Importantly, without salt treatment, the Na+ concentration was lower, and the K+ concentration was higher in the roots compared with non-colonized plants. Differential expressions of ion transporter genes were found in soybean roots after P. indica colonization. The P. indica colonization positively regulated the transcription level of PM H+-ATPase, SOS1, and SOS2. The study shows that P. indica enhances the growth and salt tolerance of soybean, providing a strategy for the agricultural production of soybean plants in saline-alkali soils.

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Modification of plasma membrane H+-ATPase in Masson pine (Pinus massoniana Lamb.) seedling roots adapting to acid deposition

Cited by 5 publications

References 85 publications

Mitogen‐activated protein kinase MxMPK3‐2 mediated phosphorylation of MxZR3.1 participates in regulating iron homoeostasis in apple rootstocks

Mitogen‐activated protein kinase MxMPK3‐2 mediated phosphorylation of MxZR3.1 participates in regulating iron homoeostasis in apple rootstocks

Overexpression of MsRCI2D and MsRCI2E Enhances Salt Tolerance in Alfalfa (Medicago sativa L.) by Stabilizing Antioxidant Activity and Regulating Ion Homeostasis

Symbiotic System Establishment between Piriformospora indica and Glycine max and Its Effects on the Antioxidant Activity and Ion-Transporter-Related Gene Expression in Soybean under Salt Stress

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