Modification of polyacrylonitrile-derived carbon nanofibers and bacteriophages on screen-printed electrodes: A portable electrochemical biosensor for rapid detection of Escherichia coli

Wang, Ruo-Fan; Wang, Reuben

doi:10.1016/j.bioelechem.2022.108229

Cited by 18 publications

(8 citation statements)

References 40 publications

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“…The incubation time for bacteria on electrodes also varied from 2 h [43] to 10 min [44], 15 min [48]. Interesting, when compared the LODs, those obtained using EIS are excellent namely 1 × 10 À 17 M tDNA [48], and 36 CFU/mL E. coli [47]. Based on the above findings, EIS immunodetection scheme using CNT requesting only 100 μL aqueous volume for detection of bacteria can be used for further detections in commercial food products.…”

Section: With Carbon-based Nanostructuresmentioning

confidence: 91%

“…Nanostructured carbon materials such as multi-walled carbon nanotubes (MWCNTs) are intensively used in the constructions of electrochemical biosensors due to their high electrical conductivity, high surface/volume, catalytic and amplification signal benefits. In this section, different sensing schemes for detection of E.coli in PBS and aqueous real samples are discussed [43,44,47,48,49] (Figure 4, Table 2).…”

Section: With Carbon-based Nanostructuresmentioning

confidence: 99%

“…CV and EIS investigations in the presence of 5 mM [Fe(CN) 6 ] 3À /4À were recorded for developed electrodes after incubation with phages and different concentration of E. coli. The biosensor was also used for the detection of E. coli (10 3 -10 6 CFU/mL) spiked in apple juice [47].…”

Section: With Carbon-based Nanostructuresmentioning

confidence: 99%

“…Silicon wafer patterned with Pt interdigitated electrodes was multi-functionalized with MWCNTs/manganese dioxide (MnO 2 )/polyaniline nanowire (PANI-NWs) nanocomposite and used in the construction of EIS genosensor for specific detection of cDNA E. coli O157:H7 F I G U R E 4 Immunodetection of E.coli strains using carbon-based materials and different electrochemical methods: chronocoulometry -A [43]; SWV -B [44]; CV-C [47] and EIS -C [47], D [48], E [49]. CNF -carbon nanofibers; IPTG -isopropyl-β-Dthiogalactopyranoside; TCEP -tris(2-carboxyethyl)phosphine hydrochloride, MPGP -4-Methoxyphenyl-β-D-galactopyranoside MPGP and Tyr -tyrosinase.…”

Section: With Carbon-based Nanostructuresmentioning

confidence: 99%

“…To summarize, five electrochemical immunodetection schemes using carbon-based nanomaterials and CC, CV, EIS, SWV measurements for identification of five E coli strains (W3110, K88, BCRC 1330, BCRC 51445, O157:H7) on conductive ITO, GC, GSPE and Pt electrodes and SPCEs are discussed in Table 2. It is thus noted for EIS studies the use of the same concentration of redox probes such as 5 mM [Fe(CN) 6 ] 3À /4À [47,48], while the tested volumes samples varied from 1 mL [43] to few mL [48] and down to 100 μL [47]. The incubation time for bacteria on electrodes also varied from 2 h [43] to 10 min [44], 15 min [48].…”

Section: With Carbon-based Nanostructuresmentioning

confidence: 99%

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Recent advances in bioelectrochemical detection of E. coli bacteria in saline buffers and spiked real samples using traditional and nanostructured active substrates

Ionescu

2023

Electroanalysis

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A rapid and cost‐effective method to specifically identify and quantify pathogenic Escherichia coli (E. coli) bacteria in aqueous samples and food products is highly recommended to avoid the degradation of human health that can unfortunately lead to fatal cases. To overcome these borderline situations, portable and easy‐to‐use screening devices are needed for the non‐expert public and confirmed by medical personnel/physicians who can quickly guide/prescribe antibiotic treatments. In such a context, nanotechnologies are very promising and useful tools due to the remarkable optical, chemical and physical properties of biocompatible nanomaterials deposited or synthesized on traditional solid electrodes that greatly improve the detection limit and the selectivity of nanostructured‐based biosensors. With this in mind, this review summarizes the latest advances in the bioelectrochemical detection of E. coli and its related products using different biosensor configurations in saline buffers and spiked real samples, namely food products (milk, fruits, vegetables), body fluids (blood, urine, swine feces) and river water.

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Section: With Carbon-based Nanostructuresmentioning

confidence: 91%

Section: With Carbon-based Nanostructuresmentioning

confidence: 99%

Section: With Carbon-based Nanostructuresmentioning

confidence: 99%

Section: With Carbon-based Nanostructuresmentioning

confidence: 99%

Section: With Carbon-based Nanostructuresmentioning

confidence: 99%

See 3 more Smart Citations

Recent advances in bioelectrochemical detection of E. coli bacteria in saline buffers and spiked real samples using traditional and nanostructured active substrates

Ionescu

2023

Electroanalysis

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Disposable electrochemical biosensors for the detection of bacteria in the light of antimicrobial resistance

C. S.,

Kini,

Singh

et al. 2024

Biotech & Bioengineering

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Persistent and inappropriate use of antibiotics is causing rife antimicrobial resistance (AMR) worldwide. Common bacterial infections are thus becoming increasingly difficult to treat without the use of last resort antibiotics. This has necessitated a situation where it is imperative to confirm the infection to be bacterial, before treating it with antimicrobial speculatively. Conventional methods of bacteria detection are either culture based which take anywhere between 24 and 96 hor require sophisticated molecular analysis equipment with libraries and trained operators. These are difficult propositions for resource limited community healthcare setups of developing or less developed countries. Customized, inexpensive, point‐of‐care (PoC) biosensors are thus being researched and developed for rapid detection of bacterial pathogens. The development and optimization of disposable sensor substrates is the first and crucial step in development of such PoC systems. The substrates should facilitate easy charge transfer, a high surface to volume ratio, be tailorable by the various bio‐conjugation chemistries, preserve the integrity of the biorecognition element, yet be inexpensive. Such sensor substrates thus need to be thoroughly investigated. Further, if such systems were made disposable, they would attain immunity to biofouling. This article discusses a few potential disposable electrochemical sensor substrates deployed for detection of bacteria for environmental and healthcare applications. The technologies have significant potential in helping reduce bacterial infections and checking AMR. This could help save lives of people succumbing to bacterial infections, as well as improve the overall quality of lives of people in low‐ and middle‐income countries.

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Electrochemical Sensing Based on Nanofibers Modified Electrodes for Application in Diagnostic, Food and Waste Water Samples

Nazari,

Kashanian,

Parnianchi

et al. 2023

ChemElectroChem

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Electrochemical sensors and biosensors are today important analytical and monitoring tools in various fields, from agriculture and the food industry to environmental and biomedical/pharmaceutical applications. In particular, the integration of nanotechnology with electrochemical sensors and biosensors to develop a new generation of sensor platforms has made enormous progress in recent years. The outstanding properties of one‐dimensional (1D) nanofibers (NFs), such as high porosity, superior mechanical properties and high specific surface area have made them attractive electrocatalysts, support materials for the immobilization of biomolecules as well as mimetic materials for sensing and biosensing applications. Moreover, the possibility of fabricating multifunctional composites based on NFs increases (bio)sensing capabilities through synergistic effects and additive properties. This review describes the progress made over the last decade in the use of multifunctional NFs‐based composites as modified electrodes for the sensing of various analytes in biomedical, food, and wastewater treatment applications. The aim of this review is to provide a comprehensive overview and a guide for researchers from different disciplines to fabricate and improve their selective NFs‐based (bio)sensor platforms for the detection of desired analytes or multi‐analytes.

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Modification of polyacrylonitrile-derived carbon nanofibers and bacteriophages on screen-printed electrodes: A portable electrochemical biosensor for rapid detection of Escherichia coli

Cited by 18 publications

References 40 publications

Recent advances in bioelectrochemical detection of E. coli bacteria in saline buffers and spiked real samples using traditional and nanostructured active substrates

Recent advances in bioelectrochemical detection of E. coli bacteria in saline buffers and spiked real samples using traditional and nanostructured active substrates

Disposable electrochemical biosensors for the detection of bacteria in the light of antimicrobial resistance

Electrochemical Sensing Based on Nanofibers Modified Electrodes for Application in Diagnostic, Food and Waste Water Samples

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