Modification of rhodamine staining allows identification of hematopoietic stem cells with preferential short-term or long-term bone marrow-repopulating ability.

Zijlmans, J. M. J. M.; Visser, J. W. M.; Kleiverda, Karin; Kluin, Philippus; Willemze, R.; Fibbe, Willem E.

doi:10.1073/pnas.92.19.8901

Cited by 94 publications

(56 citation statements)

References 20 publications

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“…Briefly, the oligo(dA)-tailed first cDNA strand from 104 rhodamine 123-dull cells [19,20] was hybridized with an excess of biotinylated poly(A) + RNA from peritoneal macrophages. Subtracted cDNA sequences were recovered by amplification with oligo(dT)-conraining primer and cloned into lambda ZAP II vector.…”

Section: Cdna Cloningmentioning

confidence: 99%

“…PCR-amplifled total cDNA was prepared from sorted murine hematopoietic stem cell subpopulations: Rho bright, Rho aull, Rho-/ Ver d~l, Rho-/Ver- [19,20], mono/granulocytes, macrophages, thymocytes and total bone marrow, by the modified procedure described earlier [24]. Amplified cDNA samples from each cell fraction were separated in 2% alkaline agarose gel and transferred to nylon membrane Hybond-N +.…”

Section: Cdna Blot Analysismentioning

confidence: 99%

“…No hybridization signal was detected in brain, muscle, kidney, liver, heart, thymus. In addition, we hybridized the MCLP probe with the Southern blot of PCR-amplified eDNA from murine bone marrow fractions enriched with stem/progenitor cells fractionated on the basis of rhodamine 123 staining: Rho bright, Rho d@, Rho-/ Ver dun and Rho-/Ver- [19,20]. No expression of MCLP mRNA was detected in the Rho-/Ver dun and Rho-/Verfractions (Fig.…”

Section: ------------mentioning

confidence: 99%

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A novel murine cathelin‐like protein expressed in bone marrow

et al. 1996

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A novel cDNA encoding a putative secreted protein was isolated from murine bone marrow. The encoded protein named MCLP (.m.urine cathelin-_like protein) was found to be highly homologous to the pig cathelin, and to four neutrophil antimicrobial polypeptides: CAP 18, indolicidin, Bac 5 and FALL-39. Secondary structure prediction studies identified a highly cationic region in the C-terminal part of prepro-MCLP with a tendency to adopt an amphipathic a-helical conformation, as observed in many antimicrobial peptides. However, no antibacterial activity was observed with the synthetic peptide corresponding to this region of MCLP.

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Section: Cdna Cloningmentioning

confidence: 99%

Section: Cdna Blot Analysismentioning

confidence: 99%

Section: ------------mentioning

confidence: 99%

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A novel murine cathelin‐like protein expressed in bone marrow

et al. 1996

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“…[44][45][46][47][48] Although such fusion events were thus considered to represent a model whereby progenitor cells express plasticity and renewal, the mechanism for this process was unknown. One candidate, however, was the ABC superfamily of active membrane transporters known to be expressed on stem/progenitor cells 49,50 and to mediate dye efflux capacity and multidrug resistance, [51][52][53][54][55][56][57][58][59][60][61][62][63][64][65] as well as influence membrane fluidity and potential. 66 Reasoning an ABC transporter that had been newly cloned, ABCB5 (ATPbinding cassette, subfamily B, member 5), might hold the key to understanding progenitor cell fusion; it was found that ABCB5 marks CD133 þ progenitor skin melanocytes and also determines the propensity for fusion.…”

Section: Stochastic Model Cancer Stem Cell Modelmentioning

confidence: 99%

Melanoma stem cells: not rare, but well done

Girouard

Murphy

2011

Laboratory Investigation

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Since the identification of self-renewing cells in the hematopoietic system, stem cells have transformed the study of medicine. Cancer biologists have identified stem-like cells in multiple malignancies, including those of solid organs. This has led to the development of a stem cell theory of cancer, which purports that a subpopulation of self-renewing tumor cells is responsible for tumorigenesis. This contrasts with the stochastic model of tumor development, which advances that all tumor cells are capable of tumor formation. Within the field of melanoma, the identity and existence of cancer stem cells has been the subject of recent debate. Much of the controversy may be traced to differences in interpretations and definitions related to the cancer stem cell theory, and the use of dissimilar methodologies to study melanoma cells. Accumulating evidence suggests that cancer stem cells may exist in melanoma, although their frequency may vary and they may be capable of phenotypic plasticity. Importantly, these primitive melanoma cells are not only capable of self-renewal and differentiation plasticity, but also may confer virulence via immune evasion and multidrug resistance, and potentially via vasculogenic mimicry and transition to migratory and metastasizing derivatives. Therapeutic targeting of melanoma stem cells and the pathways that endow them with virulence hold promise for the design of more effective strategies for amelioration and eradication of this most lethal form of skin cancer.

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“…Fluorescent dye staining approaches have also been used to define HSCs based on efflux capacity and staining profile. Rhodamine (Rho) 123 staining of bone marrow cells was used to demonstrate that high efflux of Rho123 was associated with the most primitive HSCs whereas cells that did not efflux Rho123 well and stained more brightly could only provide short-term repopulation (Bertoncello et al, 1988;Spangrude and Johnson, 1990;Zijlmans et al, 1995;Spangrude et al, 1995). Hoechst 33342 is another fluorescent dye used for isolation of HSC fractions (McAlister et al, 1990;Goodell et al, 1996).…”

Section: Phenotype and Function Of Tissue-specific Stem Cellsmentioning

confidence: 99%

Integrative molecular and developmental biology of adult stem cells

Bunting

Hawley

2003

Biology of the Cell

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Stem cells are believed to be important for regeneration of several adult tissues. Recently, adult stem cells with very broad differentiation potential have been identified although whether they represent vestigial primitive pluripotent stem cells or products of extremely rare de-differentiation events involving tissue-specific stem cells is not known. Transdifferentiation of tissue-specific stem cells across lineage boundaries has also been demonstrated but the relative inefficiency of the process in vivo, even in the presence of tissue damage, questions whether such a mechanism is of physiologic relevance. Interestingly, among adult stem cells, the capacity for lineage switching appears to be greatest in stem cells that can be cultured ex vivo for extended periods. If the normal cell fate decisions of diverse adult stem cell types could be reliably redirected at high frequency in situ, possible regenerative therapies for a wide variety of diseases could be envisioned. An integrated understanding of the transcriptional regulatory networks that comprise the various adult stem cell entities as well as the signaling pathways governing their differentiation into therapeutically useful cell types will facilitate clinical application of these exciting findings.

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Modification of rhodamine staining allows identification of hematopoietic stem cells with preferential short-term or long-term bone marrow-repopulating ability.

Abstract: We have developed a modified rhodamine (]Rho) staining procedure to study uptake and efflux in murine hematopoietic stem cells.

Cited by 94 publications

References 20 publications

A novel murine cathelin‐like protein expressed in bone marrow

A novel murine cathelin‐like protein expressed in bone marrow

Melanoma stem cells: not rare, but well done

Integrative molecular and developmental biology of adult stem cells

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